全文获取类型
收费全文 | 3120篇 |
免费 | 278篇 |
出版年
2023年 | 6篇 |
2022年 | 24篇 |
2021年 | 74篇 |
2020年 | 31篇 |
2019年 | 47篇 |
2018年 | 76篇 |
2017年 | 65篇 |
2016年 | 98篇 |
2015年 | 157篇 |
2014年 | 175篇 |
2013年 | 225篇 |
2012年 | 293篇 |
2011年 | 262篇 |
2010年 | 185篇 |
2009年 | 155篇 |
2008年 | 220篇 |
2007年 | 193篇 |
2006年 | 190篇 |
2005年 | 158篇 |
2004年 | 146篇 |
2003年 | 117篇 |
2002年 | 115篇 |
2001年 | 31篇 |
2000年 | 29篇 |
1999年 | 28篇 |
1998年 | 31篇 |
1997年 | 19篇 |
1996年 | 14篇 |
1995年 | 14篇 |
1994年 | 12篇 |
1993年 | 14篇 |
1992年 | 14篇 |
1991年 | 10篇 |
1990年 | 15篇 |
1989年 | 17篇 |
1988年 | 11篇 |
1987年 | 11篇 |
1985年 | 8篇 |
1984年 | 12篇 |
1983年 | 7篇 |
1982年 | 7篇 |
1977年 | 6篇 |
1976年 | 8篇 |
1974年 | 7篇 |
1973年 | 6篇 |
1970年 | 4篇 |
1969年 | 10篇 |
1967年 | 5篇 |
1966年 | 5篇 |
1964年 | 5篇 |
排序方式: 共有3398条查询结果,搜索用时 734 毫秒
51.
52.
53.
Federico Lucantoni Manuela Salvucci Heiko Dussmann Jochen H.M. Prehn 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》2021,1868(10):119095
BCL2 family proteins are important regulators of mitochondrial outer membrane permeabilization (MOMP). In recent years, BCL2 family proteins have also been linked to the regulation of mitochondrial bioenergetics and dynamics. Given their overexpression in breast cancer cells, we sought to explore whether two key members of this family, BCL2 and BCL(X)L impacted on mitochondrial fusion/fission processes. By employing a single cell imaging and RNA sequencing we found that overexpression of BCL2 or BCL(X)L increases mitochondrial dynamics and alters the expression profile of genes involved in this process. Collectively, our data show that overexpression of BCL2 proteins regulates mitochondrial dynamics in breast cancer tumor cells. 相似文献
54.
55.
Peroxiredoxin 1 (PRDX1) is an antioxidant enzyme that, when secreted, can act as a proinflammatory signal. Here we studied the regulation of intracellular PRDX1 by lipopolysaccharide (LPS) and interferon-gamma (IFN-γ) in the RAW 264.7 mouse macrophage cell line. While LPS or IFN-γ alone did not affect PRDX1 protein levels, their combination led to an almost complete loss of the PRDX1 dimer. This was likely mediated by the increased production of nitric oxide (NO) as it was reversed by the NO synthase inhibitor L-N-methylarginine (L-NMMA), while a NO-releasing agent decreased PRDX1 levels. Inhibition of the proteasome with MG132 also prevented the loss of the PRDX1 dimer, suggesting that the decrease is due to a NO-activated proteasomal degradation pathway. By contrast with the decrease in protein levels, LPS increased PRDX1 mRNA and this effect was amplified by IFN-γ. Two other Nrf2 target genes, thioredoxin reductase (TXNRD1) and haem oxygenase (HMOX1), were also induced by LPS but IFN-γ did not increase their expression further. This study shows that inflammation differentially regulates PRDX1 at the levels of protein stability and gene expression, and that NO plays a key role in this mechanism. 相似文献
56.
Vanessa Pitozzi Stefania Pallotta Manuela Balzi Marta Bucciolini Aldo Becciolini Piero Dolara 《Free radical research》2013,47(11):1149-1154
We used X-rays from a linear accelerator and from a low energy therapeutic source to calibrate the single cell gel electrophoresis (comet assay), a widely used method to measure DNA damage. γ-Rays from 60Co, with known efficiency in inducing DNA breakage, were used as reference. Human lymphocytes and one murine tumour cell line, F10-M3 cells, were irradiated under different experimental conditions. A similar relationship between radiation dose and induced DNA damage was obtained with γ- and X-rays. A calibration curve was constructed to convert the comet assay raw data into break frequency. The median levels of DNA breaks and oxidative damage in circulating lymphocytes from healthy volunteers were calculated to be 0.76 and 0.80 breaks/109 Da, respectively, (0.50 and 0.52 breaks/106 bp). The values of oxidative DNA damage were in the same order of magnitude as those found by others with HPLC methods. 相似文献
57.
G. Bosi A. Benatti R. Rinaldi D. Dallai C. Santini M. Carbognani 《Plant biosystems》2013,147(1):144-153
Modena, founded by the Romans (183 BC), has always been conditioned by water in all its urban history. In the city, numerous archaeobotanical investigations have been carried out in order to reconstruct the natural landscape and human–environment interactions over time. During these investigations, four archaeological sites (two Roman and two medieval) have revealed deposits with a marked character of palaeobiocoenosis, largely resulting from the natural environment surrounding the sites, due to natural “seed rain”. These deposits are characterized by widespread evidence of plants related to water, constituting a valuable archive to investigate habitats which currently have become very rare and threatened, if they have not completely disappeared. The present paper aims to reveal the peculiarities of the Roman/medieval archaeocarpological floristic lists (through a comparison with the flora over the last two centuries in the area of Modena) and highlight the possible causes explaining the presence or the demise of several taxa, considering also the palaeoecological reconstruction of the environment in which they have been found. 相似文献
58.
Marie-Pierre Lézé Pascal Marchand Denis Loquet Manuela Kogler Guillaume Le Baut 《Journal of enzyme inhibition and medicinal chemistry》2013,28(6):549-557
The present study was designed to follow our pharmacomodulation work in the field of non-steroidal aromatase inhibitors. All target compounds 12a–h and 28a–h were tested in vitro for human placental aromatase inhibition, using testosterone or androstenedione as the substrate for the aromatase enzyme and the IC50 and relative potency to aminoglutethimide data are included. A SAR study indicated that 3-[(4-fluorophenyl)(1H-imidazol-1-yl)methyl]-1-ethyl-2-methyl-1H-indole (28?g) was a highly potent and selective aromatase inhibitor with IC50 value of 0.025?μM. 28?g was also a weak inhibitor of androstenedione synthesis. 相似文献
59.