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81.
Nancy Burton-Wurster Hao Chen Rina Gendelman Michael L Jackson Lucille F Gagliardo Da Nian Gu Russell R Zelko George Lust James N MacLeod 《Matrix biology》2002,21(5):393-398
The population of fibronectins in adult mammalian cartilage includes high levels of a cartilage-specific (V+C)(-) isoform which lacks the V, III-15, and I-10 segments and thus contains a novel junction between protein segments III-14 and I-11. We report production of a monoclonal antibody specific for (V+C)(-) fibronectin without cross-recognition of V(+)C(+) and V(-)C(+) isoforms found in plasma and other tissues. Presentation of epitope to this antibody requires the III-14/I-11 junction, but the epitope itself extends beyond 14 amino acids immediately surrounding the junction site and involves a conformational change in III-14 and/or the N-terminal portion of I-11. The antibody, designated Mab 5D10 anti (V+C)(-), displays specificity for (V+C)(-) fibronectin from multiple mammalian species including humans and utility in immunoblots, immunohistochemistry, and ELISA. 相似文献
82.
Summary Seasonal fluctuations of haemoglobin (Hb) concentration in nodules and the percentage of nitrogen (N) in leaflets of rhizobium-inoculated peanuts from various planting dates, were studied under field conditions.In peanuts planted in the usual season (April, May), no correlation was found between the N and Hb concentrations during the early stages of peanut growth; however, there was a very significant correlation at later periods of plant growth. With July (out-of-season) planting there was no correlation between the Hb and N concentrations at any time.The possibility of evaluating the atmospheric nitrogen (N2) fixation rate of peanut plants under field conditions by means of Hb and N determinations was studied.Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel. 1972 Series, no. 2213-E. 相似文献
83.
84.
Epitope mapping of anti‐myelin oligodendrocyte glycoprotein (MOG) antibodies in a mouse model of multiple sclerosis: microwave‐assisted synthesis of the peptide antigens and ELISA screening
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Giulia Pacini Matthaia Ieronymaki Francesca Nuti Giuseppina Sabatino Maud Larregola Rina Aharoni Anna Maria Papini Paolo Rovero 《Journal of peptide science》2016,22(1):52-58
The role of pathologic auto‐antibodies against myelin oligodendrocyte glycoprotein (MOG) in multiple sclerosis is a highly controversial matter. As the use of animal models may enable to unravel the molecular mechanisms of the human disorder, numerous studies on multiple sclerosis are carried out using experimental autoimmune encephalomyelitis (EAE). In particular, the most extensively used EAE model is obtained by immunizing C57BL/6 mice with the immunodominant peptide MOG(35–55). In this scenario, we analyzed the anti‐MOG antibody response in this model using the recombinant refolded extracellular domain of the protein, MOG(1–117). To assess the presence of a B‐cell intramolecular epitope spreading mechanism, we tested also five synthetic peptides mapping the 1–117 sequence of MOG, including MOG(35–55). For this purpose, we cloned, expressed in Escherichia coli and on‐column refolded MOG(1–117), and we applied an optimized microwave‐assisted solid‐phase synthetic strategy to obtain the designed peptide sequences. Subsequently, we set up a solid‐phase immunoenzymatic assay testing both naïve and EAE mice sera and using MOG protein and peptides as antigenic probes. The results obtained disclose an intense IgG antibody response against both the recombinant protein and the immunizing peptide, while no response was observed against the other synthetic fragments, thus excluding the presence of an intramolecular epitope spreading mechanism. Furthermore, as the properly refolded recombinant probe is able to bind antibodies with greater efficiency compared with MOG(35–55), we hypothesize the presence of both linear and conformational epitopes on MOG(35–55) sequence. Copyright © 2015 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
85.
Elisabet Bjanes Reyna Garcia Sillas Rina Matsuda Benjamin Demarco Timothe Fettrelet Alexandra A. DeLaney Opher S. Kornfeld Bettina L. Lee Eric M. Rodríguez Lpez Daniel Grubaugh Meghan A. Wynosky-Dolfi Naomi H. Philip Elise Krespan Dorothy Tovar Leonel Joannas Daniel P. Beiting Jorge Henao-Mejia Brian C. Schaefer Kaiwen W. Chen Petr Broz Igor E. Brodsky 《PLoS pathogens》2021,17(10)
Cell death plays a critical role in inflammatory responses. During pyroptosis, inflammatory caspases cleave Gasdermin D (GSDMD) to release an N-terminal fragment that generates plasma membrane pores that mediate cell lysis and IL-1 cytokine release. Terminal cell lysis and IL-1β release following caspase activation can be uncoupled in certain cell types or in response to particular stimuli, a state termed hyperactivation. However, the factors and mechanisms that regulate terminal cell lysis downstream of GSDMD cleavage remain poorly understood. In the course of studies to define regulation of pyroptosis during Yersinia infection, we identified a line of Card19-deficient mice (Card19lxcn) whose macrophages were protected from cell lysis and showed reduced apoptosis and pyroptosis, yet had wild-type levels of caspase activation, IL-1 secretion, and GSDMD cleavage. Unexpectedly, CARD19, a mitochondrial CARD-containing protein, was not directly responsible for this, as an independently-generated CRISPR/Cas9 Card19 knockout mouse line (Card19Null) showed no defect in macrophage cell lysis. Notably, Card19 is located on chromosome 13, immediately adjacent to Ninj1, which was recently found to regulate cell lysis downstream of GSDMD activation. RNA-seq and western blotting revealed that Card19lxcn BMDMs have significantly reduced NINJ1 expression, and reconstitution of Ninj1 in Card19lxcn immortalized BMDMs restored their ability to undergo cell lysis in response to caspase-dependent cell death stimuli. Card19lxcn mice exhibited increased susceptibility to Yersinia infection, whereas independently-generated Card19Null mice did not, demonstrating that cell lysis itself plays a key role in protection against bacterial infection, and that the increased infection susceptibility of Card19lxcn mice is attributable to loss of NINJ1. Our findings identify genetic targeting of Card19 being responsible for off-target effects on the adjacent gene Ninj1, disrupting the ability of macrophages to undergo plasma membrane rupture downstream of gasdermin cleavage and impacting host survival and bacterial control during Yersinia infection. 相似文献
86.
87.
Masanari Taniguchi Kazuki Matsuura Rina Nakamura Aya Kojima Motomi Konishi Toshifumi Akizawa 《Biometals》2017,30(5):797-807
The extracellular deposition of amyloid β (Aβ) is known to be the fundamental cause of Alzheimer’s disease (AD). Aβ1-42, generated by β-secretases from the amyloid precursor protein (APP), is the main component of neuritic plaque, and the aggregation of this protein is shown to be dependent to an extent on metal ions such as copper and zinc. However, the mechanism by which Cu2+ affects the physicochemical properties of Aβ1-42 or the central nervous system is still under debate. A recent series of studies have demonstrated that both the soluble-type matrix metalloproteinases (MMP-2 and MMP-9) and the membrane-type matrix metalloproteinase (MT1-MMP) are capable of degrading Aβ peptides. MMP-7, one of the soluble-type matrix metalloproteinases, is expressed in hippocampal tissue; however, less information is available concerning the pathophysiological roles of this enzyme in the process and/or progress of Alzheimer’s disease. In this study, we examined the degradation activity of MMP-7 against various Aβ1-42’s fragment peptides and the effect of Cu2+. Although Aβ22-40 was degraded by MMP-7 regardless of Cu2+, Cu2+ inhibited the degradation of Aβ1-19, Aβ11-20, and Aβ11-29 by MMP-7. These results indicate that MMP-7 is capable of degrading Aβ1-42, and that Aβ1-42 acquired resistance against MMP-7 cleavage through Cu2+-binding and structure changes. Our results demonstrate that MMP-7 may play an important role in the defensive mechanism against the aggregation of Aβ1-42, which gives rise to the pathology of AD. 相似文献
88.
The compounds [SbCl5(R3EY)] (R = Me or Ph; E = P or As; Y = O or S) have been prepared from SbCl5 and the appropriate ligand in CH2Cl2 or CCl4 solutions, and characterised by analysis, IR, 1H, 31P{1H}, 121Sb NMR spectroscopy and conductance measurements. The [SbCl5(μ-L-L)SbCl5] L-L = Ph2P(O)CH2P(O)Ph2, Ph2P(O)(CH2)2P(O)Ph2, Ph2P(S)CH2P(S)Ph2, Ph2As(O)CH2As(O)Ph2, and o-C6H4(P(O)Ph2)2 have been synthesised and similarly characterised. The unstable [SbCl5(R3PSe)] have been prepared at low temperatures and characterised by IR spectroscopy. In solution in chlorocarbons they decompose rapidly to Se and R3PCl2. The reactions of R3SbS with SbCl5 produced R3SbCl2. 相似文献
89.
Kumar Srivastava B Soni R Patel JZ Jha S Shedage SA Gandhi N Sairam KV Pawar V Sadhwani N Mitra P Jain MR Patel PR 《Bioorganic & medicinal chemistry letters》2008,18(14):3882-3886
Facile synthesis of biaryl pyrazole sulfonamide derivative of 5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxylic acid piperidin-1-ylamide (SR141716, 1) and an investigation of the effect of replacement of the –CO group in the compound 1 by the –SO2 group in the aminopiperidine region is reported. Primary ex-vivo pharmacological testing and in vitro screening of sulfonamide derivative 2 showed the loss of CB1 receptor antagonism. 相似文献
90.
Srivastava BK Soni R Joharapurkar A Sairam KV Patel JZ Goswami A Shedage SA Kar SS Salunke RP Gugale SB Dhawas A Kadam P Mishra B Sadhwani N Unadkat VB Mitra P Jain MR Patel PR 《Bioorganic & medicinal chemistry letters》2008,18(3):963-968
Design, synthesis and conformational analysis of few imidazole and oxazole as bioisosters of 4S-(-)-3-(4-chlorophenyl)-N-methyl-N'-[(4-chlorophenyl)-sulfonyl]-4-phenyl-4,5-dihydro-1H-pyrazole-1-caboxamidine (SLV-319) 2 is reported. Computer assisted conformational analysis gave a direct clue for the loss of CB1 antagonistic activity of the ligands without a fine docking simulation for the homology model. 相似文献