全文获取类型
收费全文 | 1509篇 |
免费 | 170篇 |
出版年
2022年 | 17篇 |
2021年 | 27篇 |
2020年 | 11篇 |
2019年 | 19篇 |
2018年 | 24篇 |
2017年 | 17篇 |
2016年 | 23篇 |
2015年 | 49篇 |
2014年 | 49篇 |
2013年 | 64篇 |
2012年 | 63篇 |
2011年 | 74篇 |
2010年 | 43篇 |
2009年 | 34篇 |
2008年 | 60篇 |
2007年 | 62篇 |
2006年 | 45篇 |
2005年 | 50篇 |
2004年 | 48篇 |
2003年 | 56篇 |
2002年 | 58篇 |
2001年 | 45篇 |
2000年 | 47篇 |
1999年 | 50篇 |
1998年 | 21篇 |
1997年 | 16篇 |
1996年 | 20篇 |
1993年 | 14篇 |
1992年 | 31篇 |
1991年 | 25篇 |
1990年 | 30篇 |
1989年 | 38篇 |
1988年 | 33篇 |
1987年 | 23篇 |
1986年 | 22篇 |
1985年 | 19篇 |
1984年 | 18篇 |
1982年 | 19篇 |
1981年 | 26篇 |
1980年 | 13篇 |
1979年 | 16篇 |
1978年 | 12篇 |
1977年 | 12篇 |
1975年 | 14篇 |
1974年 | 17篇 |
1973年 | 21篇 |
1972年 | 15篇 |
1970年 | 14篇 |
1967年 | 16篇 |
1966年 | 16篇 |
排序方式: 共有1679条查询结果,搜索用时 31 毫秒
31.
32.
33.
34.
Recent progress in studies on the bacterial chromosome is summarized. Although the greatest amount of information comes from studies on Escherichia coli, reports on studies of many other bacteria are also included. A compilation of the sizes of chromosomal DNAs as determined by pulsed-field electrophoresis is given, as well as a discussion of factors that affect gene dosage, including redundancy of chromosomes on the one hand and inactivation of chromosomes on the other hand. The distinction between a large plasmid and a second chromosome is discussed. Recent information on repeated sequences and chromosomal rearrangements is presented. The growing understanding of limitations on the rearrangements that can be tolerated by bacteria and those that cannot is summarized, and the sensitive region flanking the terminator loci is described. Sources and types of genetic variation in bacteria are listed, from simple single nucleotide mutations to intragenic and intergenic recombinations. A model depicting the dynamics of the evolution and genetic activity of the bacterial chromosome is described which entails acquisition by recombination of clonal segments within the chromosome. The model is consistent with the existence of only a few genetic types of E. coli worldwide. Finally, there is a summary of recent reports on lateral genetic exchange across great taxonomic distances, yet another source of genetic variation and innovation. 相似文献
35.
We have determined the nucleotide sequences of sevenlacY alleles isolated from natural isolates ofEscherichia coli. Nucleotide heterozygosity estimates for this locus were compared to those obtained from previous studies of intraspecific variation at chromosomal loci, revealing thatlacY has unusually low synonymous site variation. The average pairwise heterozygosity of synonymous sites (Ks=0.0112+/-0.0100) is the second lowest reported and the lowest for loci that have an equivalent level of nonsynonymous variation. We consider several hypotheses to explain how different forces in evolution could act to create the observed pattern of polymorphism, including selection for translational efficiency and positive selection. Our analysis most strongly supports the hypothesis that positive selection has acted on thelacY locus inE. coli. 相似文献
36.
We have surveyed naturally occurring plasmids in strains of Bacillus subtilis and the closely related species B. mojavensis and B. licheniformis. Previous studies have failed to find host-benefitting functions for plasmids of these species, suggesting that these plasmids are nonmutualistic. Only one type of plasmid was found in each plasmid-bearing strain, suggesting that most of the plasmids infecting these Bacillus species are in the same incompatibility group. A sample of 18 plasmids from these species ranged in size from 6.9 to 16 kb, with all but 6 plasmids falling into three size groups. These groups differed in the sizes of their host ranges and geographical ranges. All but 1 of the 18 plasmids from these three host species are homologous with one another. The cryptic plasmids from these three species are far less diverse than are plasmids (from other species) that are known to benefit their bacterial hosts. The low-level diversity among these cryptic plasmids is consistent with the hypothesis that host-benefitting adaptations play an important role in fostering the coexistence of plasmid populations, but other explanations for the low-level plasmid diversity are possible. Comparison of the phylogenies of the plasmids with those of their hosts suggests that Bacillus plasmids are horizontally transferred in nature at a low rate similar to that found for the colicin plasmids of Escherichia coli. 相似文献
37.
38.
39.
A YAC contig encompassing the Treacher Collins syndrome critical region at 5q31.3-32. 总被引:1,自引:1,他引:0
下载免费PDF全文
![点击此处可从《American journal of human genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
J. Dixon A. J. Gladwin S. K. Loftus J. H. Riley R. Perveen J. J. Wasmuth R. Anand M. J. Dixon 《American journal of human genetics》1994,55(2):372-378
Treacher Collins syndrome (TCOF1) is an autosomal dominant disorder of craniofacial development the features of which include conductive hearing loss and cleft palate. Previous studies have localized the TCOF1 locus between D5S519 (proximal) and SPARC (distal), a region of 22 centirays as estimated by radiation hybrid mapping. In the current investigation we have created a contig across the TCOF1 critical region, using YAC clones. Isolation of a novel short tandem repeat polymorphism corresponding to the end of one of the YACs has allowed us to reduce the size of the critical region to approximately 840 kb, which has been covered with three nonchimeric YACs. Restriction mapping has revealed that the region contains a high density of clustered rare-cutter restriction sites, suggesting that it may contain a number of different genes. The results of the present investigation have further allowed us to confirm that the RPS14 locus lies proximal to the critical region and can thereby be excluded from a role in the pathogenesis of TCOF1, while ANX6 lies within the TCOF1 critical region and remains a potential candidate for the mutated gene. 相似文献
40.