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941.
Shiotani K Li T Miyazaki A Tsuda Y Bryant SD Ambo A Sasaki Y Lazarus LH Okada Y 《Bioorganic & medicinal chemistry letters》2006,16(22):5793-5796
Dimeric opioid analogues linked to a pyrazinone platform, 3-[Tyr/Dmt-NH(CH2)m]-6-[Tyr/Dmt-NH(CH2)n]-2(1H)-pyrazinone (m, n=3 or 4), were synthesized. The Tyr-containing compound (m=4, n=3) exhibited mu-receptor affinity (K(i)mu; 7.58 nM) comparable to that of morphine, while the Dmt derivatives exhibited considerably higher affinity (K(i)mu; 0.021-0.051 nM) with corresponding agonism (IC50=1.79-4.93 nM). Interestingly one compound (m=4, n=3) revealed modest delta-opioid agonism; the converse analogue (m=3, n=4), however, was inactive in MVD assay. 相似文献
942.
The merC gene from Acidithiobacillus ferrooxidans functions as a mercury uptake pump. MerC protein localizes in the cytoplasmic membrane of plant cells. When Arabidopsis thaliana and tobacco plants were transformed with the merC gene under the control of the Cauliflower mosaic virus 35S promoter, the resulting overexpression of merC rendered the host plants hypersensitive to Hg2+ and they accumulated approximately twice as much Hg2+ ion as the wild type plants. Thus, bacterial mercuric ion transporters such as MerC may be useful molecular tools for producing transgenic plants that hyperaccumulate Hg2+ ion. 相似文献
943.
Identification of mouse palmitoyl-coenzyme A Delta9-desaturase 总被引:5,自引:0,他引:5
Stearoyl-coenzyme A desaturase (SCD) catalyzes the desaturation of saturated fatty acids to monounsaturated fatty acids in mammalian cells. Currently, there are four known enzymatic isoforms (SCD1-SCD4) in the mouse genome. The physiological roles for multiple SCD isoforms and their substrate specificities are unknown at present. We report here distinct substrate specificities for the mouse SCD isoforms. Each SCD isoform was able to complement the ole1 mutation in Saccharomyces cerevisiae through heterologous expression of transgenic SCD. Fatty acid analysis showed that mouse SCD1, SCD2, and SCD4 desaturate both C18:0 and C16:0, whereas mouse SCD3 uses C16:0 but not C18:0. We identify SCD3 as a mammalian palmitotyl-CoA Delta9-desaturase, and its existence in mouse helps explain distinct physiological roles for each SCD isoform. 相似文献
944.
Oyama F Miyazaki H Sakamoto N Becquet C Machida Y Kaneko K Uchikawa C Suzuki T Kurosawa M Ikeda T Tamaoka A Sakurai T Nukina N 《Journal of neurochemistry》2006,98(2):518-529
Sodium channel beta4 is a very recently identified auxiliary subunit of the voltage-gated sodium channels. To find the primarily affected gene in Huntington's disease (HD) pathogenesis, we profiled HD transgenic mice using a high-density oligonucleotide array and identified beta4 as an expressed sequence tag (EST) that was significantly down-regulated in the striatum of HD model mice and patients. Reduction in beta4 started at a presymptomatic stage in HD mice, whereas other voltage-gated ion channel subunits were decreased later. In contrast, spinal cord neurons, which generate only negligible levels of expanded polyglutamine aggregates, maintained normal levels of beta4 expression even at the symptomatic stage. Overexpression of beta4 induced neurite outgrowth in Neuro2a cells, and caused a thickening of dendrites and increased density of dendritic spines in hippocampal primary neurons, indicating that beta4 modulates neurite outgrowth activities. These results suggest that down-regulation of beta4 may lead to abnormalities of sodium channel and neurite degeneration in the striatum of HD transgenic mice and patients with HD. 相似文献
945.
Permanence of a dispersal single-species population model where environment is partitioned into several patches is considered. The species not only requires some time to disperse between the patches but also has some possibility to die during its dispersion. The model is described by delay differential equations. The existence of 'super' food-rich patch is proved to be sufficient to ensure partial permanence of the model. It is also shown that partial permanence implies permanence if each food-poor patch is chained to the super food-rich patch. Furthermore, it is proven that partial persistence is ensured if there exist food-rich patches and the dispersion of the species among the patches are small. When the dispersion is large, the partial persistence is realized under relatively small dispersion time. 相似文献
946.
947.
In the circannual pupation rhythm of the varied carpet beetle, Anthrenus verbasci, entrainment to annual cycles is achieved by phase resetting of the circannual oscillator in response to photoperiodic changes. In order to examine whether a circadian system is involved in expression of the periodic pattern and phase resetting of the circannual rhythm as photoperiodic responses, we exposed larvae to light-dark cycles with a short photophase followed by a variable scotophase (the Nanda-Hamner protocol). When the cycle length (T) was a multiple of 24 h, i.e., 24, 48, or 72 h, short-day effects were clearer than when T was far from a multiple of 24 h, i.e., 36 or 60 h. Exposure to light-dark cycles of T = 36 h had effects similar to exposure to long-day cycles of T = 24 h. The magnitude of phase shifts depended on the duration and the phase of exposure to the cycles of T = 36 or 60 h. It was therefore concluded that a circadian system is involved in photoperiodic time measurement for phase resetting of the circannual oscillator of A. verbasci. 相似文献
948.
Margit Kõiv Ivika Ostonen Christina Vohla Riho Mõtlep Martin Liira Krista Lõhmus Kalle Kirsimäe Ülo Mander 《Hydrobiologia》2012,692(1):145-156
The aim of this study was to test P-enriched filter materials from a wastewater treatment experiment regarding their fertilizing efficiency in pot experiments with silver birch (Betula pendula Roth.) seedlings. Tested materials included hydrated calcium-rich oil shale ash and well-mineralised peat. A mixture of peat and hydrated ash demonstrated the best results: seedlings grown on that mixture had the highest P concentration and an optimal N:P:K ratio in leaves. Hydrated ash has a low concentration of heavy metals and almost the same composition as common lime fertilizers. The hydrated ash sediment and its combination with well-mineralised peat enriched with phosphorus in wastewater treatment filters are promising materials for forest soil fertilization. 相似文献
949.
Cho J Miyazaki S Yeh PJ Kim W Kanae S Oki T 《International journal of biometeorology》2012,56(2):411-418
Surface albedo (α) and aerodynamic roughness length (z
0), which partition surface net radiation into energy fluxes, are critical land surface properties for biosphere–atmosphere
interactions and climate variability. Previous studies suggested that canopy structure parameters influence both α and z
0; however, no field data have been reported to quantify their relationships. Here, we hypothesize that a functional relationship
between α and z
0
exists for a vegetated surface, since both land surface parameters can be conceptually related to the characteristics of
canopy structure. We test this hypothesis by using the observed data collected from 50 site-years of field measurements from
sites worldwide covering various vegetated surfaces. On the basis of these data, a negative linear relationship between α and log(z
0) was found, which is related to the canopy structural parameter. We believe that our finding is a big step toward the estimation
of z
0 with high accuracy. This can be used, for example, in the parameterization of land properties and the observation of z
0 using satellite remote sensing. 相似文献