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81.
Background
Obligate asexual reproduction is rare in the animal kingdom. Generally, asexuals are considered evolutionary dead ends that are unable to radiate. The phytophagous mite genus Bryobia contains a large number of asexual species. In this study, we investigate the origin and evolution of asexuality using samples from 111 populations in Europe, South Africa and the United States, belonging to eleven Bryobia species. We also examine intraspecific clonal diversity for one species, B. kissophila, by genotyping individuals from 61 different populations. Knowledge on the origin of asexuality and on clonal diversity can contribute to our understanding of the paradox of sex. 相似文献82.
Demarta A Küpfer M Riegel P Harf-Monteil C Tonolla M Peduzzi R Monera A José Saavedra M Martínez-Murcia A 《Systematic and applied microbiology》2008,31(4):278-286
Five Aeromonas strains, isolated from both clinical and environmental sources and characterized by a polyphasic approach, including phylogenetic analysis derived from gyrB, rpoD, and 16S rRNA gene sequencing, as well as DNA-DNA hybridization, extensive biochemical and antibiotic susceptibility tests, were recognized as members of an unknown, or undescribed, Aeromonas species. These "Aeromonas eucrenophila-like" strains were closely related to the species A. eucrenophila and Aeromonas encheleia, but they were negative for indole and acid from glycerol tests. Therefore, based on the results of the phylogenetic analyses and DNA-DNA pairing data of these strains, a novel species of the genus Aeromonas is described, for which the name Aeromonas tecta is proposed with isolate F518(T) (CECT7082(T), DSM17300(T), MDC91(T)) as the type strain. 相似文献
83.
Walter Riegel 《Review of Palaeobotany and Palynology》2008,148(2-4):73-90
The fossil record of the Palaeozoic documents one of the most dramatic changes in Phanerozoic marine primary production, although causes and effects of these changes have been the subject of rather controversial discussions. During the early Palaeozoic the marine phytoplankton experienced an enormous radiation and diversification of taxa especially among acritarchs, which was punctuated by a few extinction events possibly associated with climate changes. Phytoplankton diversity was drastically reduced at the Devonian/Carboniferous boundary, a phenomenon here designated as the “Phytoplankton Blackout”.After a lapse of about 130 million years phytoplankton diversity was gradually restored during the Late Triassic with the appearance of dinoflagellates and somewhat later of coccolithophorids and diatoms. Evidence from recent phytoplankton suggests that the dominant groups of phytoplankton differ from those of the Palaeozoic in their nutritional requirements and that fundamental changes in ocean chemistry have played an important role.The remarkable temporal coincidence of the Phytoplankton Blackout with plate tectonic processes during the assembly and breakup of Pangaea and the concomitant rise of land plants towards the end of the Devonian provide arguments that nutrient depletion in the ocean may have played a decisive role in controlling the phytoplankton blackout. Contrary arguments in favour of oceanic eutrophication, changes in life cycles of dominant phytoplankton groups or selective preservation are discussed and weighed against the scenario presented here. Metazoan evolution seems only loosely linked with the phytoplankton blackout. However, there is good agreement between phytoplankton and reef evolution throughout the Phanerozoic. 相似文献
84.
Development of EST-PCR markers and monitoring their intrapopulational genetic variation in Picea abies (L.) Karst. 总被引:6,自引:0,他引:6
R. Schubert G. Mueller-Starck R. Riegel 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(8):1223-1231
Fifteen cDNA sequences are reported for the European coniferous forest tree species Norway spruce [Picea abies (L.) Karst.], including the results of similarity searches in public electronic databases. The sequences were subsequently
employed for the design of specific primer pairs and PCR-based amplification of genomic fragments. For seven primer pairs,
polymorphic EST-PCR markers were identified among 18 trees. Their mode of inheritance was verified by analysing single-tree
offspring and studying segregation among haploid endosperms in comparison to diploid tissue. Codominant inheritance was indicated
for six markers, while one marker was apparently dominant. Variation of the six codominant EST markers was tested by genotyping
110 randomly selected trees in a Bavarian Norway spruce population. For comparison, the same trees were genotyped at 18 enzyme
coding gene loci. There were 3.33 alleles per locus for EST markers and 3.00 for isoenzyme gene markers. In general, a trend
to more even frequency distributions and larger intrapopulational variation, including observed heterozygosities, was indicated
more for EST markers than for isoenzyme gene markers. The benefits of these newly developed EST-PCR markers are outlined with
respect to population genetics and ecological genetics.
Received: 29 April 2000 / Accepted: 25 August 2000 相似文献
85.
86.
Midkine binds to anaplastic lymphoma kinase (ALK) and acts as a growth factor for different cell types 总被引:20,自引:0,他引:20
Stoica GE Kuo A Powers C Bowden ET Sale EB Riegel AT Wellstein A 《The Journal of biological chemistry》2002,277(39):35990-35998
Midkine (MK) is a developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN). To investigate the potential role of MK in tumor growth, we expressed MK in human SW-13 cells and studied receptor binding, signal transduction, and activity of MK. The MK protein stimulates soft agar colony formation in vitro and tumor growth of SW-13 cells in athymic nude mice, as well as proliferation of human endothelial cells from brain microvasculature and umbilical vein (HUVEC) in the low ng/ml range. MK binds to anaplastic lymphoma kinase (ALK), the receptor for PTN, with an apparent K(d) of 170 pm in intact cells, and this receptor binding of MK is competed by PTN with an apparent K(d) of approximately 20 pm. Monoclonal antibodies raised against the extracellular ligand-binding domain of ALK inhibit ALK receptor binding of MK as well as MK-stimulated colony formation of SW-13 cells. Furthermore, MK stimulates ALK phosphorylation in WI-38 human fibroblasts and activates PI3-kinase and MAP kinase signal transduction in WI-38, HUVEC, neuroblastoma (SH SY-5Y) and glioblastoma (U87MG) cells that express the ALK protein. We conclude that MK can act as a growth, survival, and angiogenic factor during tumorigenesis and signals through the ALK receptor. 相似文献
87.
Two sibs with duplication of 4q31-->qter due to 3:1 meiotic disjunction and mild phenotype: Clinical and cytogenetic findings in two sibs with partial duplication of 4q31.3-->qter and 21q11.2-->pter are reported. These patients are rare cases of reoccurrence of those partial trisomies due to 3:1 segregation of a maternal balanced translocation. A review of the literature reporting cases of trisomy of the 4q31-->qter segment is also made; previously reported cases mostly in addition have deletions of other chromosomes resulting from adjacent segregation of balanced translocation. The findings of our study confirm the high risk for offspring with unbalanced rearrangements in women with reciprocal translocation involving acrocentric and short chromosome segments. The study also points out that duplication of 4q31-->qter may go along with only mild phenotypic findings if there is no significant additional aneuploidy of the other chromosome involved in the rearrangement. 相似文献
88.
89.
90.