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961.
James V. Neel Robert J. Tanis Ernest C. Migliazza Richard S. Spielman Francisco Salzano William J. Oliver Marianne Morrow Sally Bachofer 《Human genetics》1977,36(1):81-107
Summary Blood samples from 509 Macushi and 623 Wapishana Amerindians of Northern Brazil and Southern Guyana have been analyzed with reference to the occurrence of rare variants and genetic polymorphisms of the following 25 systems: (i) Erythrocyte enzymes: acid phosphatase-1, adenosine deaminase, adenylate kinase-k, carbonic anhydrase-1, carbonic anhydrase-2, esterase A1,2,3, esterase D, galactose-1-phosphate uridyltransferase, isocitrate dehydrogenase, lactate dehydrogenase, malate dehydrogenase, nucleoside phosphorylase, peptidase A, peptidase B, phosphoglucomutase 1, phosphoglucomutase 2, phosphogluconate dehydrogenase, phosphohexoseisomerase, triosephosphate isomerase and (ii) Serum proteins: albumin, ceruloplasmin, haptoglobin, hemoglobin A, hemoglobin A2 and transferrin. Fifteen different rare variants were detected, involving 11 of these systems. In addition, a previously undescribed variant of ESA1,2,3 which achieves polymorphic proportions in both these tribes is described. Excluding this variant, the frequency of rare variants is 1.1/1000 in 12510 determinations in the Macushi and 4.7/1000 in 15 396 determinations in the Wapishana. The ESA1,2,3, polymorphism was not observed in 382 Makiritare, 232 Yanomama, 146 Piaroa, 404 Cayapo, 190 Kraho and 112 Moro. Irregularities in the intratribal distribution of this polymorphism in the Macushi and Wapishana render a decision as to the tribe of origin impossible at present. Gene frequencies are also given for previosly described polymorphisms of 5 systems: haptoglobin, phosphoglucomutase 1, erythrocyte acid phosphatase, esterase D, and galactose-1-phosphate-uridyl-transferase.Research supported by the National Science Foundation and the Energy Research and Development Administration. 相似文献
962.
963.
A viable, male progeny resulted from a two-year captive pairing of a male red spider monkey (A. g. panamensis) and a female black spider monkey (A. f. robustus). While the pelage colorations of the parental species are distinctive, the offspring was intermediate in appearance during
maturation. Such hybridization apparently occurs naturally in Panama; two feral spider monkeys, captured from an area of sympatry
in the Province of Panama east of the Canal Zone, were characteristic of the laboratory cross. 相似文献
964.
Richard R. Shivers 《Tissue & cell》1977,9(1):43-56
Hemocytes, which contain large cytoplasmic granules, invade the multilamellate glial sheath of ventral ganglion nerve roots of the crayfish following surgical interruption of these nerves. Electron microscopic examination of sections of plasticembedded tissues and replicas of freeze-cleaved ganglion roots reveals numerous slender cytoplasmic extensions of the hemocytes present in damaged nerve sheaths. Many of these microvillous extensions contact glial cells and filamentous extracellular masses. At sites of contact, the microvilli are flattened and occasionally electron-dense material is present in the hemocyte cytoplasm subjacent to the plasma membrane that is closely apposed to a glial cell or connective tissue. Intramembranous surfaces of hemocyte plasmalemmae exposed by freeze-fracture, exhibit particle aggregates 700–2500 Å in diameter. Individual particles are 95–105 Å in diameter. Since the particle aggregates correspond in overall dimension and position in the cell to the sites of contact of hemocyte processes with other sheath components, it is assumed that the two structures are equivalent and represent a junctional complex very similar in structure to some hemidesmosomes. Results from this study strongly suggest that granulated crustacean hemocytes, in response to surgical injury of nerves, invade the damaged nerve sheath and identify damaged glial cells and connective tissue by forming slender cytoplasmic processes which contact elements of the sheath. Tissue components contacted by the hemocytes may subsequently be phagocytosed by them. This is the first report of an invertebrate hemocyte-mediated response to tissue damage in which evidence is presented that the hemocyte may identify necrotic cells and extracellular matrix by forming junctional complexes with them. Crustacean hemocytes, therefore, are likely much more complex functionally than has been previously estimated. 相似文献
965.
Henry R. Black Samuel O. Thier Philip F. Felig James F. Jekel Joseph Belsky James L. Bernene Everett Cooper Vincent A. De Luca Jr. Martin H. Floch Marvin Garrell Pasquale E. Perillie Robert L. Piscatelli George F. Thornton 《The Yale journal of biology and medicine》1977,50(6):637-644
In July, 1975, the Departments of Internal Medicine at the Yale University School of Medicine and eight community hospitals in southern and western Connecticut formed the Yale Affiliated Hospital Program (YAHP) in Internal Medicine. The YAHP provides a planned and focused program of continuing education for medical staff and housestaff at the affiliated hospitals. Six formats for the over 1,000 rounds, lectures, and conferences given annually are used. The members of the YAHP also cooperate in housestaff and faculty recruiting, evaluation of quality of care and evaluation of the process of continuing medical education itself. This report summarizes the organization, goals and future plans of the YAHP. 相似文献
966.
Richard W. Castenholz 《Microbial ecology》1977,3(2):79-105
In the Mammoth Springs (Yellowstone National Park) waters with near neutral pH and soluble sulfide (H2S, HS–, S2–) of over 1–2 mg/liter (30–60M) are characterized by substrate covers of phototrophic bacteria (Chloroflexus and aChlorobium-like unicell) above 50C and by a blue-green alga (Spirulina labyrinthiformis) below this temperature.Synechococcus. Mastigocladus, and other blue-green algae typical of most hot springs of western North America are excluded, apparently by sulfide. The sulfide-adaptedSpirulina photosynthesized at maximum rates at 45C and at approximately 300 to 700Ein/m2/sec of visible radiation. Sulfide (0.6–1.2 mM) severely poisoned photosynthesis of nonadapted populations, but those continuously exposed to over 30M tolerated at least 1 mM without inhibition. A normal14C-HCO3 photoincorporation rate was sustained with 0.6–1 mM sulfide in the presence of DCMU (7M) or NH2OH (0.2 mM), although both of these photosystem II inhibitors prevented photoincorporation without sulfide. Other sulfur-containing compounds (S2O3
2– SO3
2–, S2O4
2– thioglycolic acid cysteine) were unable to relieve DCMU inhibition. The lowering of the photoincorporation rate by preferentially irradiating photosystem I was also relieved by sulfide. The most tenable explanation of these results is that sulfide is used as a photo-reductant of CO2, at least when photosystem II is inhibited. It is suggested that in some blue-green algae photosystem II is poisoned by a low sulfide concentration, thus making these algae sulfidedependent if they are to continue photosynthesizing in a sulfide environment. Presumably a sulfidecytochrome reductase enzyme system must be synthesized for sulfide to be used as a photo-reductant. 相似文献
967.
Michael S. DuBow Thomas Ryan Richard A. Young Thomas Blumenthal 《Molecular & general genetics : MGG》1977,153(1):39-43
Summary The Host Factor required for in vitro coliphage Q RNA replication, a heat-stable RNA binding protein present in uninfectedEscherichia coli, has been detected by both immunological and functional tests inAcinetobacter calcoaceticus, Klebsiella pneumoniae, Pseudomonas aeruginosa andPseudomonas putida. It was not detectable by these criteria inBacillus stearothermophilus, Bacillus subtilis, Caulobacter crescentus, Micrococcus lysodeikticus, Rhodopseudomonas capsulata orSaccharomyces cerevisiae. InEscherichia coli the Host Factor protein has been shown to be associated with ribosomes. It is demostrated here that this association is specific for the 30S ribosomal subunit. 相似文献
968.
Mervyn J. Bibb Richard F. Freeman David A. Hopwood 《Molecular & general genetics : MGG》1977,154(2):155-166
Summary Covalently closed circular (ccc) DNA of uniform monomer size (c. 18×106 daltons) and restriction endonuclease cleavage pattern was isolated from strains of S. coelicolor A3(2) of differing constitution in respect of the SCP1 sex factor: SCP1+, SCP1, SCP1- and NF (integrated SCP1). No such ccc DNA was found in strains of S. lividans 66 or S. parvulus ATCC 12434 whether or not they contained SCP1. These results confirmed that the 18×106 dalton plasmid is not, and does not include, SCP1, which has not so far been isolated by any of a variety of methods.Genetic data served to identify a second sex factor, SCP2, postulated to be present in SCP2+ state in the starting strains and to be capable of mutation to a variant form, SCP2*, with enhanced sex factor activity. From SCP2* strains, SCP2- cultures were isolated, at an average spontaneous frequency of about 0.8%. Crosses of pairs of SCP1- SCP2- strains were almost, but not completely, sterile; thus SCP1 and SCP2 probably contribute nearly all the fertility naturally occurring in the A3(2) strain. The two sex factors share the property of exerting an effect that may be comparable with lethal zygosis caused by F in E. coli: it is shown by SCP1-carrying strains against SCP1-, or SCP2* (but not SCP2+) strains against SCP2- and is revealed as a narrow zone of growth inhibition surrounding the plasmid-carrying culture on a background of the appropriate plasmid-negative strain.Genetically defined SCP2- strains lacked the ccc DNA found in SCP2+ and SCP2* strains. Thus this DNA apparently represents the SCP2 sex factor. A preliminary restriction endonuclease cleavage map of SCP2 was constructed, with single sites for EcoRI and HindIII, four sites for SalPI (=PstI) and more than 20 sites for SalGI (SalI). 相似文献
969.
Helmut Bertrand Frank E. Nargang Richard A. Collins Cheryl A. Zagozeski 《Molecular & general genetics : MGG》1977,153(3):247-257
Summary We have isolated twenty-six nuclear, singlegene cytochrome-deficient mutants of Neurospora crassa as an initial step toward the study of the structural components and regulatory mechanisms involved in the biogenesis of the mitochondrial cytochrome system. These mutants, together with two previously described mutants, cyt-1 and cyt-2, have been classified into six distinct groups on the basis of cytochrome phenotype: a) cytochrome aa
3
deficiency (due to mutations affecting loci designated cya); b) cytochrome b deficiency (cyb-1 locus); c) cytochrome b deficiency with a partial deficiency of cytochrome aa
3
(cyb-2 locus); d) deficiency of both cytochromes aa
3
and b (cyt loci); e) deficiency of both cytochromes aa
3
and c (cyt-2 locus); and f) partial deficiency of cytochromes aa
3
and c (cyt-12 locus).Four of seven mutations affecting cya loci have been mapped and are located on linkage groups I, II, V, and VI. It is not yet known whether these genes code for structural components of cytochrome oxidase or have a regulatory function that affects synthesis or assembly of the enzyme. The cyb-1 and cyb-2 genes are located on linkage groups V and VI, respectively, and appear to code for regulatory elements that control the biogenesis of cytochromes b and aa
3
. The positions of the cyt mutations that cause a simultaneous deficiency of cytochromes aa
3
and b are dispersed throughout the genome, except for two gene clusters on the left arm of linkage group I. Some of these mutants may be deficient in mitochondrial protein synthesis. Two mutations, cyt-2 and cyt-12, are located on linkage groups VI and II, respectively, and appear to affect genes that code for components of a regulatory system that controls the biogenesis of cytochromes aa
3
and c. 相似文献
970.
The absolute configurations of heteroxanthin ((3S,5S,6S,3′R)- 7′,8′-didehydro-5,6-dihydro-β,β-carotene-3,5,3′,6′-tetrol) ex Euglena gracilis and of diadinoxanthin ((3S,5R,6S,3′R)-5,6-epoxy-7′,8′-didehydro-5,6-dihydro-β,β-carotene-3,3′-diol) from the same source have been established by chemical reactions, hydrogen bonding studies, 1H NMR and CD. Two previously unknown carotenoids (artefacts?) from Trollius europaeus, assigned the structures (3S,5S,6S,3′S,5′R,6′R)-6,7-didehydro-5,6,5′,6′-tetrahydro-β,β -carotene-3,5,6,3′,5′-pentol and its 5R epimer, served as useful models. 相似文献