首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   6265篇
  免费   373篇
  国内免费   1篇
  2023年   58篇
  2022年   83篇
  2021年   169篇
  2020年   118篇
  2019年   142篇
  2018年   194篇
  2017年   152篇
  2016年   234篇
  2015年   332篇
  2014年   384篇
  2013年   418篇
  2012年   531篇
  2011年   537篇
  2010年   326篇
  2009年   242篇
  2008年   342篇
  2007年   365篇
  2006年   356篇
  2005年   267篇
  2004年   264篇
  2003年   227篇
  2002年   224篇
  2001年   63篇
  2000年   42篇
  1999年   38篇
  1998年   54篇
  1997年   46篇
  1996年   46篇
  1995年   36篇
  1994年   32篇
  1993年   28篇
  1992年   33篇
  1991年   22篇
  1990年   19篇
  1989年   22篇
  1988年   23篇
  1987年   9篇
  1986年   12篇
  1985年   21篇
  1984年   8篇
  1983年   11篇
  1982年   12篇
  1981年   7篇
  1979年   12篇
  1978年   10篇
  1977年   10篇
  1976年   6篇
  1975年   9篇
  1974年   9篇
  1973年   8篇
排序方式: 共有6639条查询结果,搜索用时 203 毫秒
111.
DNA methylation can be environmentally modulated and plays a role in phenotypic plasticity. To understand the role of environmentally induced epigenetic variation and its dynamics in natural populations and ecosystems, it is relevant to place studies in a real-world context. Our experimental model is the wild potato Solanum kurtzianum, a close relative of the cultivated potato S. tuberosum. It was evaluated in its natural habitat, an arid Andean region in Argentina characterised by spatial and temporal environmental fluctuations. The dynamics of phenotypic and epigenetic variability (with Methyl Sensitive Amplified Polymorphism markers, MSAP) were assayed in three genotypes across three growing seasons. These genotypes were cultivated permanently and also reciprocally transplanted between experimental gardens (EG) differing in ca. 1000 m of altitude. In two seasons, the genotypes presented differential methylation patterns associated to the EG. In the reciprocal transplants, a rapid epigenomic remodelling occurred according to the growing season. Phenotypic plasticity, both spatial (between EGs within season) and temporal (between seasons), was detected. The epigenetic and phenotypic variability was positively correlated. The lack of an evident mitotic epigenetic memory would be a common response to short-term environmental fluctuations. Thus, the environmentally induced phenotypic and epigenetic variation could contribute to populations persistence through time. These results have implications for understanding the great ecological diversity of wild potatoes.  相似文献   
112.
International Journal of Biometeorology - Lying behavior is an important indicator of the cows’ welfare and health. In this study, we evaluate the effect of the physical environment on dairy...  相似文献   
113.
Probiotics and Antimicrobial Proteins - Bacterial spores of the genus Bacillus are being evaluated as adjuvant molecules capable of improving the immune response to vaccines. In this study, we...  相似文献   
114.
Aging leads to a progressive functional decline of the immune system, rendering the elderly increasingly susceptible to disease and infection. The degree to which immune cell senescence contributes to this decline remains unclear, however, since markers that label immune cells with classical features of cellular senescence accurately and comprehensively have not been identified. Using a second‐generation fluorogenic substrate for β‐galactosidase and multi‐parameter flow cytometry, we demonstrate here that peripheral blood mononuclear cells (PBMCs) isolated from healthy humans increasingly display cells with high senescence‐associated β‐galactosidase (SA‐βGal) activity with advancing donor age. The greatest age‐associated increases were observed in CD8+ T‐cell populations, in which the fraction of cells with high SA‐βGal activity reached average levels of 64% in donors in their 60s. CD8+ T cells with high SA‐βGal activity, but not those with low SA‐βGal activity, were found to exhibit features of telomere dysfunction‐induced senescence and p16‐mediated senescence, were impaired in their ability to proliferate, developed in various T‐cell differentiation states, and had a gene expression signature consistent with the senescence state previously observed in human fibroblasts. Based on these results, we propose that senescent CD8+ T cells with classical features of cellular senescence accumulate to levels that are significantly higher than previously reported and additionally provide a simple yet robust method for the isolation and characterization of senescent CD8+ T cells with predictive potential for biological age.  相似文献   
115.
Gomes  Milena  Cazetta  Eliana  Bovendorp  Ricardo  Faria  Deborah 《Plant Ecology》2021,222(10):1143-1155

The Atlantic Forest is one of the most threatened tropical forests in the world, being drastically reduced, fragmented, and disturbed. The drastic process of anthropic occupation and exploitation of this biome has, in many cases, led to the introduction of exotic species, such as the jackfruits (Artocarpus heterophyllus). However, studies on the influence of jackfruits on the native biota are still scarce. Here we investigated the influence of fruit trees on the seed rain and early recruitment of seedlings in native remnants, comparing these patterns with those observed for a native species tapirira (Tapirira guianensis), which similarly to jackfruits, produces many fruits throughout the year, attracting a variety of frugivore species. Seed rain and seedlings observed under the jackfruits were both more abundant and equally rich to the assemblages reported under the native tapirira trees. In both species, co-specifics comprise a large part of the number of seeds (>?70%) and seedlings (>?45%) individuals and, although they attract similar seed assemblages, seedling composition diverge, particularly when co-specifics are excluded. We reported that jackfruits can attract a diverse seed and seedling assemblages, and we find no evidence that the presence of jackfruits negatively affects the arrival and initial recruitment of native plant species in the study area. These results should be analyzed with caution but considered when evaluating costs and benefits of management options to control exotic species.

  相似文献   
116.
Journal of Physiology and Biochemistry - Alendronate, a bisphosphonate used to prevent osteoporosis, stimulates osteogenesis but impairs adipogenesis. Different clinical trials suggest that the...  相似文献   
117.
118.
P-selectin glycoprotein ligand-1 (PSGL-1) is a membrane-bound glycoprotein expressed in lymphoid and myeloid cells. It is a ligand of P-, E- and L-selectin and is involved in T cell trafficking and homing to lymphoid tissues, among other functions. PSGL-1 expression has been implicated in different lymphoid malignancies, so here we aimed to evaluate the involvement of PSGL-1 in T cell lymphomagenesis and dissemination. PSGL-1 was highly expressed at the surface of human and mouse T cell leukemia and lymphoma cell lines. To assess its impact on T cell malignancies, we stably expressed human PSGL-1 (hPSGL-1) in a mouse thymic lymphoma cell line, which expresses low levels of endogenous PSGL-1 at the cell surface. hPSGL-1-expressing lymphoma cells developed subcutaneous tumors in athymic nude mice recipients faster than control empty vector or parental cells. Moreover, the kidneys, lungs and liver of tumor-bearing mice were infiltrated by hPSGL-1-expressing malignant T cells. To evaluate the role of PSGL-1 in lymphoma cell dissemination, we injected intravenously control and hPSGL-1-expressing lymphoma cells in athymic mice. Strikingly, PSGL-1 expression facilitated disease infiltration of the kidneys, as determined by histological analysis and anti-CD3 immunohistochemistry. Together, these results indicate that PSGL-1 expression promotes T cell lymphoma development and dissemination to different organs.  相似文献   
119.
During the mass settlement events of brachyuran crabs, there is a significant chance of density-dependent injury in the megalopae (last larval stage) because cannibalism can occur by larger conspecifics. Laboratory observations revealed that the appendages that are more prone to injury are eyestalks, as well as first (P1) and fifth (P5) pereiopods. The ability of Carcinus maenas megalopae to autotomize these structures and the effect of such injuries in their feeding ability and metamorphosis were investigated. All tested specimens were able to autotomize one or both of their P1 and P5, but not their eyestalks. Megalopae missing a single P1, as well as one or both P5, were able to capture and ingest prey, as well as intact specimens. Megalopae with either P1 and P5 appendages or at least one damaged eyestalk failed to ingest sufficient food to reach the nutritional threshold required to successfully metamorphose.  相似文献   
120.
We describe intergradations between the arthropod repichnial trace fossils Diplichnites gouldi (Gevers et al., 1971), Dendroidichnites Demathieu et al., 1992, and Monomorphichnus Crimes, 1970 with two distinct cubichnial traces, Gluckstadtella cooperi Savage, 1971 and Huilmuichnus santracruzensis new ichnogenus and ichnospecies. These compound ichnofossils are part of high ichnodiversity arthropod-dominated trace fossil associations from the Late Carboniferous Agua Escondida Formation of the San Rafael Basin (Argentina). The complex ichnotaxonomy of Diplichnites and the ichnospecies D. gouldi are discussed and some possible working solutions are proposed. The most likely producer of the compound trace fossils are the Pygocephalomorpha, a group of Late Paleozoic crustaceans that are recorded in nearby basins from Uruguay and Brazil. The compound trace fossils are used to infer a number of complex behaviors of pygocephalomorphs in a subaqueous setting, including caridoid (tail flip) or more primitive escape reactions, landing followed by resting and transient resting during normal walking. In addition, different gaits can be inferred from well-preserved D. gouldi, which can be correlated with contrasting trackway morphology.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号