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If the conformational transition involved in enzyme memory occurs in several elementary steps, the time constant of the overall 'slow' relaxation is mostly determined by the individual values of the rate constants pertaining to the overall transconformation. The extent of kinetic co-operativity of the enzyme reaction, however, is mostly controlled by the degree of reversibility of the elementary steps of the conformational transition. There is then no simple relation between the time scale of the 'slow' transition and the extent of kinetic co-operativity of the enzyme reaction. A slow transition of about 10(-3) s-1 is therefore perfectly compatible with a strong positive or negative co-operativity and in particular with the negative co-operativity observed with wheat germ hexokinase LI. The relationship that has been established recently [Pettersson, G. (1986) Eur. J. Biochem. 154, 167-170] between the 'slow' enzyme relaxation and the extent of kinetic co-operativity holds only in the specific case where the transconformation occurs in one step. Owing to the possible occurrence of a multistep conformation change, the lack of this relationship means nothing as to the validity, or the invalidity, of the concept of mnemonical transition. More informative than the time scale of the 'slow' transition is its dependence with respect to glucose and glucose 6-phosphate, which both react with the enzyme. The effect of reaction products on the modulation of kinetic co-operativity is also of cardinal importance in the diagnosis of enzyme memory. Since an alternative model has been recently proposed by Pettersson (cited above) to explain the mechanistic origin of kinetic co-operativity of monomeric enzymes, the effect of products on the kinetic co-operativity predicted by this alternative model has been studied theoretically, in order to determine whether it is consistent with the experimental results obtained with wheat germ hexokinase LI. This analysis shows that the predictions of this model are in total disagreement with both the predictions of the mnemonical model and the experimental results obtained with wheat germ hexokinase LI, as well as with other enzymes. This alternative model cannot therefore be considered as a sensible explanation of the mechanistic origin of co-operativity of monomeric enzymes. It is therefore concluded that the mnemonical model which rests on numerous experimental results, obtained by different research groups, on different enzymes is the simplest and most likely explanation of the kinetic subtleties displayed by some monomeric enzymes, and in particular wheat germ hexokinase LI. 相似文献
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Kinetic study of yeast hexokinase. 1. Steady-state kinetics 总被引:3,自引:0,他引:3
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Gánti's chemoton model (Gánti, T., 2002. On the early evolution of biological periodicity. Cell. Biol. Int. 26, 729) is considered as an iconic example of a minimal protocell including three key subsystems: membrane, metabolism and information. The three subsystems are connected through stoichiometrical coupling which ensures the existence of a replication cycle for the chemoton. Our detailed exploration of a version of this model indicates that it displays a wide range of complex dynamics, from regularity to chaos. Here, we report the presence of a very rich set of dynamical patterns potentially displayed by a protocell as described by this implementation of a chemoton-like model. The implications for early cellular evolution and synthesis of artificial cells are discussed. 相似文献
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The effects of inorganic mercury (HgII) and methylmercury (MeHg) on the colonization of artificial substrates by periphytic
diatoms were studied using indoor freshwater microcosms. These consisted of a mixed biotope– water column + natural sediment
– with rooted macrophyte cuttings (Elodea densa) and benthic bivalve molluscs (Corbicula fluminea).The periphyton was collected
on glass slides in the water column after 34and 71 days. The two Hg sources were introduced either by daily additions to the
water column, or once at the beginning into the sediment, using two nominal concentrations: water column, 0.5 μgL-1 and 2 μg L-1 for both compounds: sediment, 0.5 mg kg-1 (fw) and 2 mgkg-1 (fw) for MeHg and 1 mg kg-1 (fw) and 10 mgkg-1 (fw) for HgII. Several complementary criteria were used to analyse the structural and functional perturbations induced: cell
density, species richness, diatom size, relative abundance. Exposure to MeHg added to the water column resulted in reduced
cell density and changes in species composition with enhancement of e.g. Fallacia pygmaea or Nitzschia palea; inorganic Hg
had less effect on the population structure. After contamination via the sediment, the effects of the two compounds were less
pronounced than for the water source.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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