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61.
Myriam Ghorbel Siwar Baklouti-Gargouri Rim Keskes Nozha Chakroun Afifa Sellami Faiza Fakhfakh Leila Ammar-Keskes 《Gene》2014
The relationship between male infertility and microdeletions in the Y chromosome that remove multiple genes varies among countries and populations. The aim of this study was to investigate the different types of Chromodomain protein, Y-linked 1 (CDY1) gene deletions and their effect on male infertility and spermatogenesis in Tunisian men. A total of 241 infertile men with different spermatogenic impairments and 115 fertile men were included in this study. We determined the prevalence of CDY1a and CDY1b copy deletions by PCR-RFLP using PvuII as restriction endonuclease. Results: Among the 356 Tunisian individuals, 93.25% had the two copies (CDY1a and CDY1b) of CDY gene (91.2% in infertile patients and 97.3% in fertile men). We also found that deletion of CDY1b was significantly more frequent in infertile patients (azoo/oligospermic and normospermic) than in fertile men (7% vs 1.7% respectively; p value = 0.02). However, deletion of CDY1a copy was very rare, and was detected in only one fertile man and four normospermic infertile patients. Our findings showed that deletion of CDY1b copy gene is a significant risk factor for male infertility independent of sperm concentration, whereas deletion of CDY1a gene seems to have no effect on fertility in the Tunisian population. 相似文献
62.
Myriam Ghorbel Siwar Baklouti-Gargouri Rim Keskes Nozha Chakroun Afifa Sellami Faiza Fakhfakh Leila Ammar-Keskes 《Gene》2014
The relationship between male infertility and AZFc micro-deletions that remove multiple genes of the Y chromosome varies among countries and populations. The purpose of this study was to analyze the prevalence and the characteristics of different Deleted in azoospermia (DAZ) gene copy deletions and their association with spermatogenic failure and male infertility in Tunisian men. 241 infertile men (30.7% azoospermic (n = 74), 31.5% oligozoospermic (n = 76) and 37.7% normozoospermic (n = 91)) and 115 fertile healthy males who fathered at least one child were included in the study. Three DAZ-specific single nucleotide variant loci and six bi-allelic DAZ-SNVs (I–VI) were analyzed using polymerase chain reaction (PCR)–restriction fragment length polymorphism and PCR. Our findings showed high frequencies of infertile men (73.85%) and controls (78.26%) having only three DAZ gene copies (DAZ1/DAZ2/DAZ3 or DAZ1/DAZ3/DAZ4 variants); so deletion of DAZ2 or DAZ4 were frequent both in infertile (36.5% and 37.3%, respectively) and fertile groups (33.9% and 44.3%, respectively) and removing DAZ4 copy was significantly more frequent in oligospermic than in normospermic men (p = 0.04) in infertile group. We also report for the first time that simultaneous deletion of both DAZ2 and DAZ4 copies was significantly more common in infertile men (12.4%) than in fertile men (4.3%) (p = 0.01). However, deletions of DAZ1/DAZ2 and DAZ3/DAZ4 clusters were very rare. Analysis of DAZ gene copies in Tunisian population, suggested that the simultaneous deletion of DAZ2 and DAZ4 gene copies is associated with male infertility, and that oligospermia seems to be promoted by removing DAZ4 copy. 相似文献
63.
Najla Trabelsi Hanen Falleh Ines Jallali Alia Ben Daly Hafedh Hajlaoui Abderrazek Smaoui Chedly Abdelly Riadh Ksouri 《Acta Physiologiae Plantarum》2012,34(1):87-96
Changes in phenolic composition and biological activities were investigated in different Limoniastrum monopetalum L. organs. For that, 80% aqueous acetone extracts were used to estimate total phenolic contents and their antioxidant activities
were evaluated using DPPH· and O2·− radical scavenging activities and reducing power. The efficiency of organ extracts was tested against human pathogen strains.
Ultimately, acid hydrolysis of all organs was subjected to RP-HPLC for phenolic identification. Results showed that flower
extracts exhibited the highest polyphenol (65.42 GAE/g DW) and flavonoid (35.36 CE/g DW) contents. Stems were enriched in
condensed tannin content (21.4 mg CE/g DW) and displayed the best antiradical activities and the highest reducing power. Besides,
stem and gall extracts showed the highest efficiency against pathogenic bacteria as compared with those of flower. Concerning
the antifungal test, a slight activity was found in gall extracts. The RP-HPLC showed a difference in phenolic compounds that
varied as function of organ. In fact, the major phenolic compound varied as function of organ. Results suggest that L. monopetalum could be a promising source of biomolecules for therapeutic and nutraceutical industries and the difference between organs
may be related to their physiological role. 相似文献
64.
Gwena?lle Le Blay Riadh Hammami Christophe Lacroix Isma?l Fliss 《Probiotics and antimicrobial proteins》2012,4(4):250-258
Listeria monocytogenes is responsible for severe foodborne infections, which can be life-threatening especially for infants and elderly populations. The emergence of antibiotic-resistant pathogens has stimulated the search for new strategies, such as the use of bacteriocins, to prevent or cure foodborne infectious diseases in the intestine. In this study, we evaluated the efficacy of the bacteriocin pediocin PA-1 from Pediococcus acidilactici UL5 to inhibit Listeria ivanovii, used as a surrogate for L. monocytogenes, under physiological conditions of the terminal ileum, simulated in a continuous in vitro fermentation model. A fecal sample from a healthy adult was immobilized and propagated for 30?days in a continuous stirred tank reactor, fed with a nutritive medium simulating the ileal chime (pH 7.5). After reaching a pseudo-steady state, the reactor was inoculated five times with L. ivanovii to reach a final concentration of 107 CFU/ml within the reactor. Two spikes of L. ivanovii without adjunction of pediocin PA-1 served as control assays, and three other spikes were done to test the effects of three concentrations of pediocin PA-1 corresponding to 2, 3, and 5× the minimum inhibitory concentration (MIC) active against L. ivanovii. The concentration of L. ivanovii in the reactor was followed for 8?h using the PALCAM selective medium. The different groups of commensal bacteria were enumerated on selective medium or using fluorescence in situ hybridization. Our data showed that pediocin PA-1 is stable in the ileum conditions and that it is able to exert its inhibition activity against L. ivanovii in a dose-dependent manner. The addition of pediocin PA-1 at 5?×?MIC induced a complete disappearance of L. ivanovii (5 log reduction) within 5?h, compared to a reduction of 2 logs, corresponding to the washout phenomenon, when no pediocin PA-1 was added. Reduction of 0.8 and 1.3 logs within 8?h was also obtained with the addition of 2 and 3?×?MIC, respectively. The same experiment has shown that addition of pediocin-PA1 in the reactor had a negligible effect on the balance of commensal bacteria. 相似文献
65.
The effect of cadmium on growth and contents of glutathione (GSH) and phytochelatins (PCs) were investigated in roots and
leaves of tomato plants (Lycopersicon esculentum Mill. cv. 63/5 F1). The accumulation of Cd increased with external Cd concentrations and was considerably higher in roots
than in leaves. Dry mass production decreased under Cd treatment especially in leaves. In both roots and leaves, exposure
to Cd caused an appreciable decline in GSH contents and increase in PCs synthesis proportional to Cd concentrations in the
growth medium. At the same Cd concentration, PCs production was higher in roots than in leaves. The implication of glutathione
in PC synthesis was strongly suggested by the use of buthionine sulfoximine (BSO). The major fraction of Cd accumulated by
tomato roots was in the form of a Cd-PCs complex. 相似文献
66.
The aim of this work was to study the effect of antimicrobial peptides: divergicin M35 and nisin A on Listeria monocytogenes LSD 530 potassium (K+) channels: ATP-sensitive (KATP), calcium-activated (BKCa), and depolarization-activated (Kv) types. Increase on K+ efflux and inhibition of cellular growth were observed after adding K+ channel activators pinacidil, NS1619, and cromakalim to divergicin M35. Increase in K+ efflux from log-phase cells was about 18 ± 1.1, 11 ± 0.63, and nmol mg−1 of cell dry weight (CDW) for pinacidil and NS1619, respectively, over the efflux obtained with divergicin M35 alone. Increases
in K+ efflux obtained by adding the same K+ channel activators to nisin A fit a completely different profile. Divergicin M35 activates K+ channels, particularly of the Kv and BKCa types and to a lesser extent the KATP type, causing K+ efflux and consequently cell death. 相似文献
67.
Effects of low doses of Li carbonate injected into mice. Functional changes in kidney seem to be related to the oxidative status 总被引:1,自引:0,他引:1
Effects of daily injections of lithium carbonate (20, 40 or 80 mg/kg body weight) during 14 and 28 days were investigated in Wistar mice. Attention was paid (1) to changes in concentrations of lithium, creatinine and urea in serum, (2) to level of oxidative stress by measuring lipids peroxidation level and catalase, superoxide-dismutase and glutathione-peroxidase activities, and (3) to changes in the histological structure of brain. The first intraperitoneal injection was followed by a transitory peak of lithium in the blood, reaching 0.25 mM and 1.1 mM and disappearing 6 and 12 h later for the 20 and 80 mg/kg doses, respectively. From the first to the last day of treatment, lithium concentrations in the blood, measured 12 h after the injections, increased from 0 to 0.11 mM (20 mg/kg dose) or 0.25 mM (80 mg/kg dose). The 80 mg/kg treatment induced a renal insufficiency evidenced by an increase of blood creatinine and urea levels. Lithium treatment was found to trigger an oxidative stress in kidney, but not in brain. In kidney, the lipid peroxidation level (TBARS) and the superoxide dismutase and catalase activities were increased. No change in glutathione peroxidase activity was detected. Histology of the brain cortex revealed interesting modifications: thicker neuronal cells and a denser network of dendrites, as compared to controls. 相似文献
68.
Falleh H Ksouri R Chaieb K Karray-Bouraoui N Trabelsi N Boulaaba M Abdelly C 《Comptes rendus biologies》2008,331(5):372-379
Polyphenols are bioactive molecules exhibiting a lot of scientific attention due to their multiple biological activities. This study compared phenolic contents and antioxidant activity in Cynara cardunculus L. organs and focus on leaf phenolic compounds identification by RP-HPLC and their antibacterial activity. The analyzed organs exhibited different total polyphenol contents (7-14.8 mg GAE g(-1) DW). Leaf and seed phenolic contents were similar and two times higher than those in flowers. The same tendency was observed for the amount of flavonoids and tannins. However, seed extracts displayed the highest DPPH. scavenging ability with the lowest IC50 value (23 microg ml(-1)), followed by leaves and flowers (over 50 microg ml(-1)). In contrast, leaves showed the highest capacity to quench superoxide (IC50: 1 microg ml(-1)) as compared to seeds (6 microg ml(-1)). In addition, cardoon leaves were efficient to inhibit growth of pathogenic bacteria mainly against Staphylococcus aureus and Escherichia coli. The identification of phenolic compounds from leaves revealed that syringic and trans-cinnamic acids were the major molecules. 相似文献
69.
Bourgou S Ksouri R Bellila A Skandrani I Falleh H Marzouk B 《Comptes rendus biologies》2008,331(1):48-55
In the present investigation, methanolic extracts from shoots and roots of Tunisian Nigella sativa were assayed for their antioxidant and antimutagenic activities. The phenolic composition of the methanolic extracts was determined by RP-HPLC. The predominant phenolic compound was vanillic acid with a mean concentration of 143.21 and 89.94 mg per 100 g dry weight of shoots and roots, respectively. Shoots and roots showed comparable and strong superoxide scavenger activity; however, shoots exhibited higher DPPH radical scavenging, reducing and chelating activities than roots. Mutagenic and antimutagenic activities were determined by using the Ames test. Shoots and roots demonstrated important antimutagenic effects. Roots exhibited stronger activity than shoots with an inhibition percentage of 71.32%. 相似文献
70.
Multiple unusual locations of hydatid cysts including bladder, psoas muscle and liver 总被引:1,自引:0,他引:1
Feki W Ghozzi S Khiari R Ghorbel J Elarbi H Khouni H Ben Rais N 《Parasitology international》2008,57(1):83-86
Our case concerns 66-year-old female with a multiple unusual locations of hydatid cysts including bladder, psoas muscle and liver. Coexistence of hydatid cysts in these localizations has not been previously reported. The diagnosis of vesical hydatid cyst was facilitated by the coexistence of other echinococcosis locations. Treatment consists of the excision of the cysts in the same session without any postoperative anthelmintic drugs. In a two-year follow-up no recurrence has occurred. 相似文献