全文获取类型
收费全文 | 162篇 |
免费 | 19篇 |
出版年
2024年 | 1篇 |
2022年 | 1篇 |
2021年 | 4篇 |
2020年 | 4篇 |
2019年 | 5篇 |
2018年 | 7篇 |
2017年 | 6篇 |
2016年 | 9篇 |
2015年 | 8篇 |
2014年 | 11篇 |
2013年 | 12篇 |
2012年 | 13篇 |
2011年 | 10篇 |
2010年 | 5篇 |
2009年 | 7篇 |
2008年 | 10篇 |
2007年 | 6篇 |
2006年 | 6篇 |
2005年 | 8篇 |
2004年 | 7篇 |
2003年 | 1篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1998年 | 3篇 |
1995年 | 1篇 |
1989年 | 2篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 3篇 |
1976年 | 1篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1958年 | 1篇 |
1952年 | 1篇 |
1939年 | 1篇 |
1934年 | 1篇 |
1929年 | 1篇 |
1927年 | 1篇 |
1919年 | 1篇 |
1918年 | 1篇 |
1913年 | 1篇 |
1910年 | 1篇 |
1900年 | 1篇 |
1888年 | 1篇 |
排序方式: 共有181条查询结果,搜索用时 328 毫秒
131.
132.
Camilo Elber Vital Andrea Giordano Eduardo de Almeida Soares Thomas Christopher Rhys Williams Rosilene Oliveira Mesquita Pedro Marcus Pereira Vidigal Amanda de Santana Lopes Túlio Gomes Pacheco Marcelo Rogalski Humberto Josué de Oliveira Ramos Marcelo Ehlers Loureiro 《Plant molecular biology》2017,94(6):577-594
133.
134.
Assembly of the secretion pores GspD,Wza and CsgG into bacterial outer membranes does not require the Omp85 proteins BamA or TamA
下载免费PDF全文
![点击此处可从《Molecular microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Rhys A. Dunstan Iain D. Hay Jonathan J. Wilksch Ralf B. Schittenhelm Anthony W. Purcell Joan Clark Adam Costin Georg Ramm Richard A. Strugnell Trevor Lithgow 《Molecular microbiology》2015,97(4):616-629
In Gram‐negative bacteria, β‐barrel proteins are integrated into the outer membrane by the β‐barrel assembly machinery, with key components of the machinery being the Omp85 family members BamA and TamA. Recent crystal structures and cryo‐electron microscopy show a diverse set of secretion pores in Gram‐negative bacteria, with α‐helix (Wza and GspD) or β‐strand (CsgG) transmembrane segments in the outer membrane. We developed assays to measure the assembly of three distinct secretion pores that mediate protein (GspD), curli fibre (CsgG) and capsular polysaccharide (Wza) secretion by bacteria and show that depletion of BamA and TamA does not diminish the assembly of Wza, GspD or CsgG. Like the well characterised pilotins for GspD and other secretins, small periplasmic proteins enhance the assembly of the CsgG β‐barrel. We discuss a model for integral protein assembly into the bacterial outer membrane, focusing on the commonalities and differences in the assembly of Wza, GspD and CsgG. 相似文献
135.
Skylarks are common birds on arable farmland throughout Britain and have recently been implicated in grazing damage to sugar-beet seedlings in Eastern England. Their feeding habits and their relationship to variations in food supplies were described. 相似文献
136.
137.
Mowbray CE Bell AS Clarke NP Collins M Jones RM Lane CA Liu WL Newman SD Paradowski M Schenck EJ Selby MD Swain NA Williams DH 《Bioorganic & medicinal chemistry letters》2011,21(21):6596-6602
We describe the development of novel benzimidazoles as small molecule histamine H4 receptor (H4R) antagonists and their profiling in rat early toxicity studies. The discovery and optimisation of a second series of pyrimidine based antagonists is then described culminating in the identification of the clinical development candidate 13 (PF-3893787). The pre-clinical profile of 13 (PF-3893787) is presented including the development of a translatable biomarker. Our pragmatic approach to target selection, safety assessment, and testing for efficacy faced numerous challenges and we share a number of lessons which the team learned and which will assist us and others in future drug discovery projects. 相似文献
138.
Masood MA Selby MD Bell AS Mansfield AC Gardner M Smith GF Lane C Kenyon-Edwards H Osborne R Jones RM Liu WL Brown CD Clarke N Perrucio F Mowbray CE 《Bioorganic & medicinal chemistry letters》2011,21(21):6591-6595
We describe the identification of a potent, selective lead series that shows antagonism against the human histamine H4 receptor from thirteen actives identified in an HTS as part of a hit to lead program. By focusing on ligand efficiency and concurrently using a diversity based approach, compounds based around 2,4-diaminopyrimidine were identified with compound 25 being quickly shown to be a good lead. It also had the highest ligand efficiency in the series. 相似文献
139.
Cuthbert R Taggart MA Prakash V Saini M Swarup D Upreti S Mateo R Chakraborty SS Deori P Green RE 《PloS one》2011,6(5):e19069
Contamination of their carrion food supply with the non-steroidal anti-inflammatory drug diclofenac has caused rapid population declines across the Indian subcontinent of three species of Gyps vultures endemic to South Asia. The governments of India, Pakistan and Nepal took action in 2006 to prevent the veterinary use of diclofenac on domesticated livestock, the route by which contamination occurs. We analyse data from three surveys of the prevalence and concentration of diclofenac residues in carcasses of domesticated ungulates in India, carried out before and after the implementation of a ban on veterinary use. There was little change in the prevalence and concentration of diclofenac between a survey before the ban and one conducted soon after its implementation, with the percentage of carcasses containing diclofenac in these surveys estimated at 10.8 and 10.7%, respectively. However, both the prevalence and concentration of diclofenac had fallen markedly 7-31 months after the implementation of the ban, with the true prevalence in this third survey estimated at 6.5%. Modelling of the impact of this reduction in diclofenac on the expected rate of decline of the oriental white-backed vulture (Gyps bengalensis) in India indicates that the decline rate has decreased to 40% of the rate before the ban, but is still likely to be rapid (about 18% year(-1)). Hence, further efforts to remove diclofenac from vulture food are still needed if the future recovery or successful reintroduction of vultures is to be feasible. 相似文献
140.
Caleb González Joe Christian J Ray Michael Manhart Rhys M Adams Dmitry Nevozhay Alexandre V Morozov Gábor Balázsi 《Molecular systems biology》2015,11(8)
Stress response genes and their regulators form networks that underlie drug resistance. These networks often have an inherent tradeoff: their expression is costly in the absence of stress, but beneficial in stress. They can quickly emerge in the genomes of infectious microbes and cancer cells, protecting them from treatment. Yet, the evolution of stress resistance networks is not well understood. Here, we use a two-component synthetic gene circuit integrated into the budding yeast genome to model experimentally the adaptation of a stress response module and its host genome in three different scenarios. In agreement with computational predictions, we find that: (i) intra-module mutations target and eliminate the module if it confers only cost without any benefit to the cell; (ii) intra- and extra-module mutations jointly activate the module if it is potentially beneficial and confers no cost; and (iii) a few specific mutations repeatedly fine-tune the module''s noisy response if it has excessive costs and/or insufficient benefits. Overall, these findings reveal how the timing and mechanisms of stress response network evolution depend on the environment. 相似文献