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排序方式: 共有233条查询结果,搜索用时 15 毫秒
91.
Jungsun Kim John P. Hoffman R. Katherine Alpaugh Andrew D. Rhim Maximilian Reichert Ben Z. Stanger Emma E. Furth Antonia R. Sepulveda Chao-Xing Yuan Kyoung-Jae Won Greg Donahue Jessica Sands Andrew A. Gumbs Kenneth S. Zaret 《Cell reports》2013,3(6):2088-2099
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92.
The extracellular matrix (ECM) provides an essential structural framework for cell attachment, proliferation, and differentiation,
and undergoes progressive changes during senescence. To investigate changes in protein expression in the extracellular matrix
between young and senescent fibroblasts, we compared proteomic data (LTQ-FT) with cDNA microarray results. The peptide counts
from the proteomics analysis were used to evaluate the level of ECM protein expression by young cells and senescent cells,
and ECM protein expression data were compared with the microarray data. After completing the comparative analysis, we grouped
the genes into four categories. Class I included genes with increased expression levels in both analyses, while class IV contained
genes with reduced expression in both analyses. Class II and Class III contained genes with an inconsistent expression pattern.
Finally, we validated the comparative analysis results by examining the expression level of the specific gene from each category
using Western blot analysis and semiquantitative RT-PCR. Our results demonstrate that comparative analysis can be used to
identify differentially expressed genes. 相似文献
93.
Amraoui F Krida G Bouattour A Rhim A Daaboub J Harrat Z Boubidi SC Tijane M Sarih M Failloux AB 《PloS one》2012,7(5):e36757
West Nile fever (WNF) and Rift Valley fever (RVF) are emerging diseases causing epidemics outside their natural range of distribution. West Nile virus (WNV) circulates widely and harmlessly in the old world among birds as amplifying hosts, and horses and humans as accidental dead-end hosts. Rift Valley fever virus (RVFV) re-emerges periodically in Africa causing massive outbreaks. In the Maghreb, eco-climatic and entomologic conditions are favourable for WNV and RVFV emergence. Both viruses are transmitted by mosquitoes belonging to the Culex pipiens complex. We evaluated the ability of different populations of Cx. pipiens from North Africa to transmit WNV and the avirulent RVFV Clone 13 strain. Mosquitoes collected in Algeria, Morocco, and Tunisia during the summer 2010 were experimentally infected with WNV and RVFV Clone 13 strain at titers of 10(7.8) and 10(8.5) plaque forming units/mL, respectively. Disseminated infection and transmission rates were estimated 14-21 days following the exposure to the infectious blood-meal. We show that 14 days after exposure to WNV, all mosquito st developed a high disseminated infection and were able to excrete infectious saliva. However, only 69.2% of mosquito strains developed a disseminated infection with RVFV Clone 13 strain, and among them, 77.8% were able to deliver virus through saliva. Thus, Cx. pipiens from the Maghreb are efficient experimental vectors to transmit WNV and to a lesser extent, RVFV Clone 13 strain. The epidemiologic importance of our findings should be considered in the light of other parameters related to mosquito ecology and biology. 相似文献
94.
EMT and dissemination precede pancreatic tumor formation 总被引:2,自引:0,他引:2
Rhim AD Mirek ET Aiello NM Maitra A Bailey JM McAllister F Reichert M Beatty GL Rustgi AK Vonderheide RH Leach SD Stanger BZ 《Cell》2012,148(1-2):349-361
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95.
96.
We studied the seasonal changes in territorial behaviour of hazel grouse (Bonasa bonasia) from December 1999 to July 2001 in a temperate forest of South Korea. The study was based on the use of radio telemetry. Territory size and degree of overlap increased after the breeding period, and index of advertisement and border disputes decreased. In spring, the territory was defended by both males and females. During autumn and winter, this behaviour was relaxed to such a degree that hazel grouse cannot be considered territorial, but as forming a home range. 相似文献
97.
98.
99.
We previously showed that Asb-4 and Asb-17 is uniquely expressed in developing male germ cells. A recent report showed that Asb-9 is specifically expressed in the kidney and testes; however, detailed expression patterns in developing germ cells have not been shown. Northern blot analysis in various tissues demonstrated that mAsb-9 was strongly expressed in the testes. Expression analysis by RT-PCR and Northern blot in developing mouse testes indicates that mAsb-9 is expressed from the fourth week after birth to adulthood, with the highest expression in round spermatids. Expression sites were further localized by in situ hybridization in the testes. Pachytene spermatocytes and spermatids expressed mAsb-9 but spermatogonia and generated spermatozoa did not. This study reveals that mAsb-9 could be a specific marker of active spermatogenesis and would be useful for studies of male germ cell development. 相似文献
100.
Kim BW Choi M Kim YS Park H Lee HR Yun CO Kim EJ Choi JS Kim S Rhim H Kaang BK Son H 《Cellular signalling》2008,20(4):714-725
The present study was undertaken to characterize neuronal activity-dependent expression and release of vascular endothelial growth factor (VEGF) from rat hippocampal neurons and its contribution to neuronal functions. Increased levels of VEGF164 mRNA were evident both in cultured neurons and slices, but not astrocytes, following membrane depolarization with KCl. Activity-dependent expression of VEGF, as well as its release, was dependent on the activation of the N-methyl-d-aspartate receptors or L-type voltage-activated calcium channels. A brief (10 min) application of recombinant VEGF165 to neurons elicited a slow rise in cytosolic Ca2+ in a VEGFR2 dependent manner. The VEGF-induced Ca2+ responses required Ca2+ influx, phospholipase Cgamma and Ca2+ stores. An inhibitor of transient receptor potential canonical channels reduced the VEGF-induced Ca2+ responses by 50%, suggesting the involvement of transient receptor potential canonical channels in the VEGF-mediated responses. The same brief stimulus with VEGF led to long-term synaptic enhancement dependent on protein synthesis. VEGF had prominent effects on the activation calcium/calmodulin protein kinase II and cAMP responsive element binding protein as well as extracellular signal-regulated protein kinase and mammalian target of rapamycin-all in a VEGFR2 dependent manner. Our findings suggest that VEGF released from neuronal cells plays a local role in Ca2+ influx and synaptic transmission that may influence the generation of long-term changes in synaptic efficacy. 相似文献