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排序方式: 共有575条查询结果,搜索用时 250 毫秒
571.
Galber Rodrigues Araujo Emília Rezende Vaz Patricia Tiemi Fujimura Jo?o Eurico Fonseca Lucélia Maria de Lima Helena Canh?o Gabriela Venturini Karina Helena Morais Cardozo Valdemir Melechco Carvalho Marcelo Henrique Napimoga Luiz Ricardo Goulart Jo?o Gon?alves Carlos Ueira-Vieira 《Arthritis research & therapy》2015,17(1)
IntroductionRheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that affects around 1 % of the human population worldwide. RA diagnosis can be difficult as there is no definitive test for its detection. Therefore, the aim of this study was to identify biomarkers that could be used for RA diagnosis.MethodsSera from a collagen-induced arthritis mouse model were used to select potential biomarkers for RA diagnosis by phage display technology. In silico and in vitro analyses were performed to characterize and validate the selected peptides. Samples were classified into three groups: RA; two other immune-mediated rheumatic diseases (systemic lupus erythematosus (SLE) and ankylosing spondylitis (AS)); and healthy controls (HC). Enzyme-linked immunosorbent assay (ELISA) was carried out to determine antibody levels, and diagnostic parameters were determined by constructing receiver operating characteristic curves. Mass spectrometry and Western blot were performed to identify the putative autoantigen that was mimicked by a highly reactive mimotope.ResultsAfter three rounds of selection, 14 clones were obtained and tested for immunoreactivity analysis against sera from RA and HC groups. The phage-fused peptide with the highest immunoreactivity (M12) was synthesized, and was able to efficiently discriminate RA patients from SLE, AS and HCs (p < 0.0001) by ELISA. The specificity and sensitivity of anti-M12 antibodies for RA diagnosis were 91 % and 84.3 %, respectively. The M12 peptide was identified as one that mimics a predicted antigenic site of the carbonic anhydrase III (CAIII) protein, a ubiquitous biomarker that has been identified in patients with other diseases.ConclusionM12 is the first peptide associated with the CAIII protein that may be used as an antigen for antibody detection to aid in RA diagnosis with high sensitivity and specificity. 相似文献
572.
Natália Coelho Couto de Azevedo Fernandes Paloma Martins do Nascimento Angélica M. Sánchez-Sarmiento Rodrigo A. Ressio Cinthya dos Santos Cirqueira Cristina Takami Kanamura Júlia de Carvalho Silvana Mello Pereira da Silva Amanda Rezende Peruchi Julio César de Souza Junior Zelinda Maria Braga Hirano José Luiz Catão-Dias 《Journal of medical primatology》2020,49(2):65-70
573.
Ana Paula da C. Marques Sandra Maria V. L. Oliveira Grazielli R. Rezende Dayane A. Melo Sonia M. Fernandes-Fitts Elenir Rose J. C. Pontes Maria da Glória Bonecini-Almeida Zoilo P. Camargo Anamaria M. M. Paniago 《Mycopathologia》2013,176(1-2):41-47
Epidemiological studies of paracoccidioidomycosis have been based on surveys achieved with intradermal tests, and paracoccidioidin is the most common antigen used in most cases. The glycoprotein of 43-kDa (gp43) has been used in intradermal tests. It is the most antigenic component of Paracoccidioides brasiliensis, and it provides greater specificity to evaluate infection for this fungus. In this study, the prevalence of P. brasiliensis infection was estimated with intradermal tests involving gp43 for 695 people in rural Central-West Brazil. The infection rate was 45.8 % (95 % CI = 42.1–49.5), and the average age of those infected was 45.8 ± 18.2 years. The prevalence did not show gender-based differences but increased with age. The results demonstrate the importance of P. brasiliensis infection in rural settlements and the early exposure of children in the region to the fungus. Despite the high antigenicity and specificity of gp43, its usage must be standardized, so that epidemiological surveys will be comparable and more accurately reflect P. brasiliensis infection in endemic areas. 相似文献
574.
575.
Rezende Priscila Costa Miranda Camilla Fracalossi Débora Machado Hayashi Leila Seiffert Walter Quadros do Nascimento Vieira Felipe Schleder Delano Dias 《Journal of applied phycology》2022,34(5):2603-2614
Journal of Applied Phycology - This study evaluated the combined use of the brown seaweeds Sargassum filipendula (S) and Undaria pinnatifida (U) dry biomass as feed additive for Pacific white... 相似文献