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101.
Adorian Taida Juliana Jamali Hadi Farsani Hamed Ghafari Darvishi Paria Hasanpour Soleiman Bagheri Tahereh Roozbehfar Reza 《Probiotics and antimicrobial proteins》2019,11(1):248-255
Probiotics and Antimicrobial Proteins - This study was conducted to evaluate different doses of two species of Bacillus (Bacillus licheniformis and Bacillus subtilis), on growth parameters,... 相似文献
102.
Tanhaeian Abbas Jaafari Mahmoud Reza Ahmadi Farajollah Shahriari Vakili‐Ghartavol Roghayyeh Sekhavati Mohammad Hadi 《Probiotics and antimicrobial proteins》2019,11(3):1034-1041
Probiotics and Antimicrobial Proteins - Nowadays, cancer remains a major cause of death affecting millions of people. Currently, the antimicrobial peptides (AMPs) as potent anticancer therapeutic... 相似文献
103.
Yousefi R Imani M Ardestani SK Saboury AA Gheibi N Ranjbar B 《Acta biochimica et biophysica Sinica》2007,39(10):795-802
Calprotectin, a heterodimeric complex belonging to the S 100 protein family, has been found predominantly in the cytosolic fraction of neutrophils. In the present study, human calprotectin was purified from neutrophils using two-step ion exchange chromatography. The purified protein was used for circular dichroism study and fluorescence analysis in the presence of calcium and zinc at physiological concentrations, as well as for assessment of its inhibitory activity on the K562 leukemia cell line. The thermal stability of the protein at pH 7.0 (physiological pH) and 8.0 (similar to intestinal pH) was also compared. The results of cell proliferation analysis revealed that human calprotectin initiated growth inhibition of the tumor cells in a dose- dependent manner. The intrinsic fluorescence emission spectra of human calprotectin (50 ktg/ml) in the presence of calcium and zinc ions show a reduction in fluorescence intensity, reflecting a conformational change within the protein with exposure of aromatic residues to the protein surface that is important for the biological function of calprotectin. The far ultraviolet-circular dichroism spectra of human calprotectin in the presence of calcium and zinc ions at physiological concentrations show a decrease in the m-helical content of the protein and an increase in [3- and other structures. Our results also show that increasing the pH level from 7.0 to 8.0 leads to a marked elevation in the thermal stability of human calprotectin, indicating a significant role for pH in the stability of calprotectin in the gut. 相似文献
104.
Perspectives for the treatment of brucellosis in the 21st century: the Ioannina recommendations 总被引:3,自引:0,他引:3
105.
Narges Marjani Maryam Dareini Maryam Asadzade-Lotfabad Mahtab Pejhan Parisa Mokaberi Zeinab Amiri-Tehranizadeh Mohammad Reza Saberi Jamshidkhan Chamani 《Luminescence》2022,37(2):310-322
With advances in new drug therapies, it is essential to understand the interactions between drugs and target molecules. In this study, we applied multiple spectroscopic techniques including absorbance, fluorescence, circular dichroism spectroscopy, viscosity, thermal melting, calorimetric, and molecular dynamics (MD) simulation to study the interaction between 2-Ethyl-5-(4-methylphenyl) pyramido pyrazole ophthalazine trione (PPF) and calf thymus DNA (ct DNA) in the absence or presence of histone H1. PPF exhibits a high binding affinity towards ct DNA in binary and ternary systems. In addition, the result for the binding constant was observed within the range 104 M−1 achieved through fluorescence quenching data, while the values for enthalpy and entropy changes for ct DNA–PPF and (ct DNA–H1) PPF complexes were measured to be −72.54 kJ.mol−1, −161.14 J.mol−1 K−1, −85.34 kJ.mol−1, and −19.023 J.mol−1 K−1, respectively. Furthermore, in accordance with circular dichroism spectra, the inducement of ct DNA structural changes was observed during binding of PPF and H1 in binary and ternary system forms. The essential roles of hydrogen bonding and van der Waals forces throughout the interaction were suggested using thermodynamic parameters. According to the obtained data, the interaction mode of ct DNA–PPF and (ct DNA–H1) PPF complexes was intercalation binding. Suggested by the MD simulation study, the ct DNA–H1 complex caused a reduction in the stability of the DNA structure in the presence or absence of ligand, which demonstrated that PPF as an intercalating agent can further distort the structure. The information achieved from this study will be very helpful in understanding the effects of PPF on the conformational state of ct DNA in the absence or presence of the H1 molecule, which seems to be quite significant for clarifying the mechanisms of action and its pharmacokinetics. 相似文献
106.
Mirjalili Seyedsina Tohidi Moghadam Tahereh H. Sajedi Reza 《Plasmonics (Norwell, Mass.)》2022,17(3):1269-1277
Plasmonics - Any excess or lack of human serum albumin (HSA) in urine should be considered a warning for renal or cardiovascular diseases. This report proposes a rapid and straightforward method... 相似文献
107.
Islam Mohammad Rakibul Yasir Fahim Antor Md. Rakib Hossain Turja Mahmudul Hassan Rahman Ashikur Khan Md. Rezaul Hoque Reza Md. Taslim 《Plasmonics (Norwell, Mass.)》2022,17(1):131-141
Plasmonics - This research illustrates a parametric study based on surface plasmon resonance of a slightly gold-coated photonic crystal fiber (PCF). In verifying sensing accuracy, the proposed... 相似文献
108.
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110.
Homa Mollaei Reza Safaralizadeh Zeinab Rostami 《Journal of cellular physiology》2019,234(8):12369-12384
Despite the recent progress in cancer management approaches, the mortality rate of cancer is still growing and there are lots of challenges in the clinics in terms of novel therapeutics. MicroRNAs (miRNA) are regulatory small noncoding RNAs and are already confirmed to have a great role in regulating gene expression level by targeting multiple molecules that affect cell physiology and disease development. Recently, miRNAs have been introduced as promising therapeutic targets for cancer treatment. Regulatory potential of tumor suppressor miRNAs, which enables regulation of entire signaling networks within the cells, makes them an interesting option for developing cancer therapeutics. In this regard, over recent decades, scientists have aimed at developing powerful and safe targeting approaches to restore these suppressive miRNAs in cancerous cells. The present review summarizes the function of miRNAs in tumor development and presents recent findings on how miRNAs have served as therapeutic agents against cancer, with a special focus on tumor suppressor miRNAs (mimics). Moreover, the latest investigations on the therapeutic strategies of miRNA delivery have been presented. 相似文献