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431.
Ivonne I. Vega‐Ruiz Jos Alfredo Castillo‐Guerrero Guillermo Fernndez 《Journal of Field Ornithology》2021,92(1):18-29
Understanding breeding phenology and success can elucidate population dynamics, which is especially important for species in need of conservation. We describe the factors affecting the breeding biology of American Oystercatchers (Haematopus palliatus frazari) at El Rancho Island, a critical site that contains ~ 7% of the total estimated population, on the coast of Sinaloa, Mexico. We monitored 192 nests over four years (2016–2019). The breeding season lasted from March to June and mean laying dates differed among years, with the mean laying date in 2019 an average of 20 days earlier than in 2016. Clutch sizes decreased as the breeding season progressed. Both breeding success and productivity differed among years, with the lowest values in 2016 (30% hatching success and 0.6 chicks/nest) and the highest in 2019 (66% hatching success and 1.2 chicks/nest). Hatching success was affected by year, laying date, type of habitat, and distance to the high tide line. American Oystercatchers that laid eggs earlier in the season, used mixed marsh and dune habitat, and with nests relatively close to the waterline (< 50 m) had greater breeding success. Overall, however, the breeding success of American Oystercatchers was low and influenced by a combination of several intrinsic and extrinsic factors. Management measures may be required to increase breeding success and ensure the conservation of this subspecies. 相似文献
432.
433.
Reyna Lucero Camacho Morales Arelí Durón Castellanos Roberto Zazueta-Sandoval 《Antonie van Leeuwenhoek》2010,98(4):437-445
Two inducible NADP+-dependent glycerol dehydrogenase (GlcDH) activities were identified in Mucor circinelloides strain YR-1. One of these, denoted iGlcDH2, was specifically induced by n-decanol when it was used as sole carbon source in the culture medium, and the second, denoted iGlcDH1, was induced by alcohols
and aliphatic or aromatic hydrocarbons when glycerol was used as the only substrate. iGlcDH2 was found to have a much broader
substrate specificity than iGlcDH1, with a low activity as an ethanol dehydrogenase with NAD+ or NADP+ as cofactor. Both isozymes showed an optimum pH for activity of 9.0 in Tris-HCl buffer and are subject to carbon catabolite
repression. In contrast, the constitutive NADP+-dependent glycerol dehydrogenases (GlcDHI, II, and III) were only present in cell extracts when the fungus was grown in glycolytic
carbon sources or glycerol under oxygenation, and their optimum pH was 7.0 in Tris-HCl buffer. In addition to these five NADP+-dependent glycerol dehydrogenases, a NAD+-dependent alcohol dehydrogenase is also present in glycerol or n-decanol medium; this enzyme was found to have weak activity as a glycerol dehydrogenase. 相似文献
434.
Cooksey Kristena Sanz Crickette Ebombi Thierry Fabrice Massamba Jean Marie Teberd Prospère Magema Espoir Abea Gaston Peralejo Juan Salvador Ortega Kienast Ivonne Stephens Colleen Morgan David 《International journal of primatology》2020,41(2):181-202
International Journal of Primatology - Socioecological variables influence the rate and nature of encounters between conspecific primate groups. Both social (i.e., type of encounter) and ecological... 相似文献
435.
Mario A. Torres-Acosta Viviana Clavijo Christopher Vaglio Andrés F. González-Barrios Martha J. Vives-Flórez Marco Rito-Palomares 《Biotechnology progress》2019,35(5):e2852
Poultry products are one of the major transmission media of Salmonella enteritidis to humans. A promising alternative to reduce the load of Salmonella in poultry are bacteriophages. Elsewhere, a mixture of six bacteriophages has been used successfully, but large-scale production would be necessary to supply potential poultry market and costs analyses have not been calculated yet. For this, a powerful tool to predict production costs is bioprocess modeling coupled with economic analyses. This work aims to model the scaled-up production of a six bacteriophages mixture based on a laboratory/pilot-scale production using Biosolve Process. For the model construction, a combination of experimental and reported data was applied, in which different production alternatives and the range of 1–100% of the Colombian poultry market (at broiler's farm and slaughterhouse) were analyzed. Results indicate that the best cost-effective process configuration/scale is to use one bioreactor (156 L) for the six bacteriophages, then a 0.45 μm filtration for removal of biomass, and a 0.22 μm filtration for sterility; this to supply the 35% of the market size for broiler farms (equivalent to 210 million chickens). This configuration gives a production cost per chicken of US$ 0.02. Additionally, a sensitivity analysis and a theoretical contrast for understanding the impact that titer and recovery have on production scale determined that titer affects the most the cost and requires optimization. The present works serves as a first, and required, approach for the development of phage therapy products that are alternatives to present-day pathogens control strategies. 相似文献
436.
437.
Daniel Talamás‐Lara José Luis Rosales‐Encina Bibiana Chávez‐Munguía Karla Acosta‐Virgen Verónica Ivonne Hernández‐Ramírez Lizbeth Salazar‐Villatoro Martha Espinosa‐Cantellano Adolfo Martínez‐Palomo Patricia Talamás‐Rohana 《The Journal of eukaryotic microbiology》2020,67(4):491-504
Early steps of tissue invasion by Entamoeba histolytica are mediated by adhesion and migration through matrix components such as fibronectin with the participation of the actin cytoskeleton. Striking differences in their produced structures, movement, and migration were found. These observations suggest differential changes in their ability to organize the actin cytoskeleton and, therefore, to modify its morphology after adhesion to fibronectin. To understand these observations, we explore deeper the cytoskeleton pathway of E. histolytica compared to Entamoeba dispar, analyzing the activation and involvement of actin cytoskeleton regulatory proteins such as small GTPases (Rho, Rac1 and Cdc42), myosin IB, paxillin, alpha‐actinin, and ARP2/3 during interaction with fibronectin. Results showed a higher activation of Rac1 in E. histolytica compared to E. dispar, while Cdc42 and RhoA were equally activated in both amebae; besides, variations in the amount of myosin IB, paxillin, and ARP2/3 were detected among these species, coinciding and reflected in formation of lamellipodia in E. histolytica and filopodia in E. dispar. These could partially explain the higher invasive capacity of E. histolytica compared to E. dispar, due to its pleomorphic ability, high motility, migration, activation, and abundance of proteins involved in the cytoskeleton arrangement. 相似文献
438.
439.
Ivonne J. Garzón-Orduña Daniel Rafael Miranda-Esquivel Mariano Donato 《Journal of Biogeography》2008,35(5):903-913
Aim To demonstrate that parsimony analysis of endemicity (PAE) is not analogous to a cladistic biogeographical analysis. Location We used six data sets from previously published studies from around the world. Methods In order to test the efficiency of PAE in recovering historical relationships among areas, we performed an empirical comparison of nodes recovered with PAE, primary Brooks parsimony analysis (BPA), and an event‐based method using three models (maximum codivergence, reconciled trees, and the default model of the treefitter program) for six data sets. We measured the performance of PAE in recovering historical area relationships by counting the number and examining the content of nodes recovered by PAE and by historical methods. The dispersal/vicariance ratio was calculated to assess the prevalence of dispersal or vicariance in each reconstruction and its relationship to the performance of PAE. Results Our results show that PAE recovers an average of 17.25% of historical nodes. PAE and BPA tend to provide similar results; however, in relation to the event‐based models, PAE performance was poor under all the tested scenarios. Although in some cases PAE reconstructions are more resolved than historical reconstructions, this does not necessarily mean that PAE produces more informative answers. These additional nodes correspond to unsupported statements that are based solely on the distributional data of taxa and not on their phylogenetic history. In other words, these nodes were not found by the historical methods, which take phylogenetics into account. The number of historical nodes recovered using PAE was in general negatively correlated with the dispersal/vicariance ratio. Main conclusions Our results show that PAE is unable to recover historical patterns and therefore does not fit into the current paradigm of historical biogeography. These findings raise doubts regarding conclusions derived from biogeographical studies that interpret PAE trees as area cladograms. We acknowledge that PAE aims to describe but does not explain the current distribution of organisms. It is therefore a useful tool in other biogeographical or ecological analyses for exploring the distribution of taxa or for establishing hypotheses of primary homology between areas. 相似文献
440.
EFFECTS OF PRE-IRRADIATION ON ISOGENEIC AND SEMI-ISOGENEIC CFU GROWTH: A STUDY ON GENETIC RESISTANCE
The genetic resistance to a parental bone marrow transplant as demonstrated, when transplantation was performed early after irradiation, failed to occur if the interval between irradiation and transplantation was increased to 4 days. A similar radiation induced weakening of genetic resistance to a parental bone marrow graft in spleen and bone marrow could be demonstrated in mice, which had been irradiated with a sublethal dose at 7 days prior to the lethal irradiation and transplantation. The pre-irradiation of the recipient with a sublethal dose induced an enhancement of the growth in spleen and bone marrow of isogeneic transplanted CFU. The pre-irradiation of a single tibia also resulted in a significant weakening of the resistance in the spleen. The experiments with partial body pre-irradiation suggested a local effect of the pre-irradiation, but it could be shown that the enhanced CFU growth is not caused by an enhanced seeding of CFU in pre-irradiated bone marrow. The role of microenvironment in the phenomenon of genetic resistance is discussed. 相似文献