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11.
Reyhaneh Moradi-Marjaneh Seyed Mahdi Hassanian Mehraneh Mehramiz Majid Rezayi Gordon A. Ferns Majid Khazaei Amir Avan 《Journal of cellular physiology》2019,234(7):10072-10079
Reactive oxygen species (ROS) are produced by mitochondria during metabolism. In physiological states, the production of ROS and their elimination by antioxidants are kept in balance. However, in pathological states, elevated levels of ROS interact with susceptible cellular target compounds including lipids, proteins, and DNA and deregulate oncogenic signaling pathways that are involved in colorectal cancer (CRC) carcinogenesis. Although antioxidant compounds have been successfully used in the treatment of CRC as prevention approaches, they have also been shown in some cases to promote disease progression. In this review, we focus on the role of ROS in gastrointestinal homeostasis, CRC progression, diagnosis, and therapy with particular emphasis on ROS-stimulated pathways. 相似文献
12.
Nicholas Harris Faina Yurgenzon Kogan Gabriela Il'kova Stefan Juhas Orly Lahmy Yevgeniya I. Gregor Juraj Koppel Regina Zhuk Paul Gregor 《Biochimica et Biophysica Acta (BBA)/General Subjects》2014
Background
Small molecule inhibitors of biologically important protein–glycosaminoglycan (GAG) interactions have yet to be identified.Methods
Compound libraries were screened in an assay of L-selectin–IgG binding to heparin (a species of heparan sulfate [HS-GAG]). Hits were validated, IC-50s established and direct binding of hits to HS-GAGs was investigated by incubating compounds alone with heparin. Selectivity of inhibitors was assessed in 11 different protein-GAG binding assays. Anti-inflammatory activity of selected compounds was evaluated in animal models.Results
Screening identified a number of structurally-diverse planar aromatic cationic amines. Scaffolds similar to known GAG binders, chloroquine and tilorone, were also identified. Inhibitors displayed activity also against bovine kidney heparan sulfate. Direct binding of compounds to GAGs was verified by incubating compounds with heparin alone. Selectivity of inhibitors was demonstrated in a panel of 11 heparin binding proteins, including selectins, chemokines (IL-8, IP-10), Beta Amyloid and cytokines (VEGF, IL-6). A number of selected lead compounds showed dose-dependent efficacy in peritonitis, paw edema and delayed type hypersensitivity.Conclusions
A new class of compounds, SMIGs, inhibits protein–GAG interaction by direct binding to GAGs. Although their IC-50s were in the low micro-molar range, SMIGs binding to HS-GAGs appeared to be stable in physiological conditions, indicating high avidity binding. SMIGs may interfere with major checkpoints for inflammatory and autoimmune events.General significance
SMIGs are a class of structurally-diverse planar aromatic cationic amines that have an unusual mode of action — inhibiting protein–GAG interactions via direct and stable binding to GAGs. SMIGs may have therapeutic potential in inflammatory and autoimmune disorders. 相似文献13.
14.
Comella P Pontvianne F Lahmy S Vignols F Barbezier N Debures A Jobet E Brugidou E Echeverria M Sáez-Vásquez J 《Nucleic acids research》2008,36(4):1163-1175
Ribonuclease III (RNaseIII) is responsible for processing and maturation of RNA precursors into functional rRNA, mRNA and other small RNA. In contrast to bacterial and yeast cells, higher eukaryotes contain at least three classes of RNaseIII, including class IV or dicer-like proteins. Here, we describe the functional characterization of AtRTL2, an Arabidopsis thaliana RNaseIII-like protein that belongs to a small family of genes distinct from the dicer family. We demonstrate that AtRTL2 is required for 3'external transcribed spacer (ETS) cleavage of the pre-rRNA in vivo. AtRTL2 localizes in the nucleus and cytoplasm, a nuclear export signal (NES) in the N-terminal sequence probably controlling AtRTL2 cellular localization. The modeled 3D structure of the RNaseIII domain of AtRTL2 is similar to the bacterial RNaseIII domain, suggesting a comparable catalytic mechanism. However, unlike bacterial RNaseIII, the AtRTL2 protein forms a highly salt-resistant homodimer that is only disrupted on treatment with DTT. These data indicate that AtRTL2 may use a dimeric mechanism to cleave double-stranded RNA, but unlike bacterial or yeast RNase III proteins, AtRTL2 forms homodimers through formation of disulfide bonds, suggesting that redox conditions may operate to regulate the activity of RNaseIII. 相似文献
15.
Identification of a 67 kDa protein that binds specifically to the pre-rRNA primary processing site in a higher plant. 下载免费PDF全文
In radish pre-rRNA primary processing cleavage occurs at a UUUUCGCGC element (motif P) mapped in the 5'-external transcribed spacer (Delcasso-Tremousaygue et al., 1988). Significantly, motif P is part of a cluster of homologous elements including three UUUUCCGG elements (motifs A123) and a single UUUUGCCCC element (motif B). Here we used the EMSA to identify in radish extracts an RNA-binding activity, NF C, that specifically interacts with the pre-rRNA A123BP sequence. Using different RNA probes and competitors we show that NF C recognises a 38 base RNA sequence including the 3'-end of motif A3 and motifs B and P. NF C binds to poly U, but not to poly A, poly C or poly G. Therefore we used poly (U) Sepharose chromatography as a final step to obtain pure NF C fractions. These, analysed by SDS-PAGE, revealed two major polypeptides of 67 and 60 kDa. According to UV cross-linking analysis the 67 kDa polypeptide corresponds to NF C activity, while the 60 kDa species is a proteolysed form of this protein. We also showed that NF C is enriched in nuclear extracts. Based on its stringent RNA substrate specificity and its nuclear localisation we propose that NF C is involved in pre-rRNA primary processing in plants. 相似文献
16.
Phytosomal‐curcumin antagonizes cell growth and migration,induced by thrombin through AMP‐Kinase in breast cancer 下载免费PDF全文
Milad Hashemzehi Reihane Behnam‐Rassouli Seyed Mahdi Hassanian Maryam Moradi‐Binabaj Reyhaneh Moradi‐Marjaneh Farzad Rahmani Hamid Fiuji Mahdi Jamili Mahdi Mirahmadi Nadia Boromand Mehran Piran Mohieddin Jafari Amirhossein Sahebkar Amir Avan Majid Khazaei 《Journal of cellular biochemistry》2018,119(7):5996-6007
17.
Normal human dermal fibroblasts have a limited life-span in vitro and stop proliferation after a fixed number of cell divisions. This process by which cells stop proliferation is called senescence. Senescence is also characterized by a decrease in the total cell number. In this study, we characterized an increase in cell death in normal human dermal fibroblasts in vitro as a function of increasing cell passage. With increasing passage, human fibroblasts showed an increase in the number of dead cells and increased DNA fragmentation as determined by flow cytometry. Serial passage of human fibroblasts also resulted in mitochondrial dysfunction, represented by a loss of mitochondrial membrane potential. The apoptotic markers caspase-3 and cytochrome c were both found to increase in senescent cells. These results suggest the activation of an apoptotic pathway within a population of human fibroblasts as a function of cell passage. 相似文献
18.
Fardin?Barati Javad?JavanbakhtEmail author Farajollah?Adib-Hashemi Ehsan?Hosseini Reyhaneh?Safaeie Mojtaba?Rajabian Mostafa?Razmjoo Reza?Sedaghat Mehdi?Aghamohammad Hassan 《Diagnostic pathology》2013,8(1):826
Background
Kombucha, a fermented tea (KT) is claimed to possess many beneficial properties. The aim of this study was to evaluate clinical and histopathological alterations of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rat.Methods
In present study 24 Wister -albino rats weighing 150–200 g were selected and divided to two treatment groups as Nitrofurazone ointment (0.2%) and Kombucha tea. Subsequently, the anesthesia was exerted by Ketamin hydrochloride 10% (40 mg/kg) and Xylasine (2 mg/kg) through intra muscular (IM) route. Furthermore, upon preparation of dorsal region of the animal for surgery, a piece of full-thickness skin removed (2?×?2 cm). In order to comparing wounds healing clinically and histologically, once every four days from the commencement, the wounds were photographed and the healed surface was measured by Scion image software.Result
The clinical findings indicated that the Kombucha fungus resulted in precipitating healing than Nitrofurazone; however, it was not significant (p?>?0.05). In order to pathological comparing of wound healing process, several wound biopsies were taken on 4, 8, 12, 16 and 20th days. Additionally, the histopathological results demonstrated that there was inflammation in Nitrofurazone group through twelveth day, somehow the epithelium was formed and abundant vessels were visible. Although on 16th day and the previous days the healing condition of Kombucha fungus was considered as minimal rate, revealing it is similar to Nitrofurazone group on 20th day.Conclusions
To wrap up. These observations suggest that the Kombucha fungus healing quality was rapid from 12th day to the end of the research, whereas no significant difference was observed.Virtual slide
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/110740713610219619.
Reyhaneh Darsouei Mojtaba Hosseini Mohammad Ghadamyari 《Biocontrol Science and Technology》2017,27(7):867-885
The current work investigated the immune response of Spodoptera exigua Hübner (Lepidoptera: Noctuidae) when challenged with two entomopathogenic nematodes (EPNs), Steinernema carpocapsae (Weiser) and Heterorhabditis bacteriophora (Poinar). The cellular and humoral defences were considered in this study. The haemocytes were observed around H. bacteriophora, but no haemocyte was found around S. carpocapsae. In larvae treated with H. bacteriophora and S. carpocapsae, total haemocyte counts (THCs) reached maximum levels at 4 and 12 hours post-injection (hpi), respectively, but decreased with the proliferation of symbiotic bacteria. In the humoral defence, there was no significant difference between EPNs on phenoloxidase (PO) activity. Phospholipase A2 (PLA2) and protease activity levels in the initial time post-injection were higher in the larvae treated with S. carpocapsae than in H. bacteriophora. In the following, the roles of symbiotic bacteria and axenic infective juveniles (IJs) in suppressing the immune system were studied separately. Maximum THC levels were observed in larvae treated with axenic nematodes and minimum THC levels were recorded in the live Xenorhabdus nematophila treatment. In the humoral defence, PLA2 activity with axenic S. carpocapsae was suppressed at 4 hpi, while in monoxenic S. carpocapsae the PLA2 level was increased to the maximum amount at 8 hpi. PO activity with monoxenic S. carpocapsae decreased gradually by 4 hpi; in live X. nematophila, it decreased from 0.5 to 16 hpi, while in axenic S. carpocapsae, it increased slowly from 0.5 to 16 hpi. The current work showed the synergistic effect of nematode and its bacterium in the suppression of the immune system and highlighted the role of the symbiont in inhibition of immune responses. 相似文献
20.
Lahmy S Guilleminot J Schmit AC Pelletier G Chaboute ME Devic M 《The Plant journal : for cell and molecular biology》2007,52(4):615-626
During Arabidopsis embryogenesis, the control of division between daughter cells is critical for pattern formation. Two embryo-defective (emb) mutant lines named quatre-quart (qqt) were characterized by forward and reverse genetics. The terminal arrest of qqt1 and qqt2 embryos was at the octant stage, just prior to the round of periclinal divisions that establishes the dermatogen stage . Homozygous embryos of a weaker allele of qqt1 were able to divide further, resulting in aberrant periclinal divisions. These phenotypic analyses support an essential role of the QQT proteins in the correct formation of the tangential divisions. That an important proportion of qqt1 embryos were arrested prior to the octant stage indicated a more general role in cell division. The analysis of QQT1 and QQT2 genes revealed that they belong to a small subgroup of the large family encoding ATP/GTP binding proteins, and are widely conserved among plants, vertebrates and Archaea. We showed that QQT1 and QQT2 proteins interact with each other in a yeast two-hybrid system, and that QQT1 and QQT2 tagged by distinct fluorescent probes colocalize with microtubules during mitosis, in agreement with their potential role in cell division and their mutant phenotype. We propose that QQT1 and QQT2 proteins participate in the organization of microtubules during cell division, and that this function is essential for the correct development of the early embryo. 相似文献