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111.
112.
Shailly Anand Sukanya Lal Utkarsh Sood Vipin Gupta Gauri Garg Dhingra Renu Solanki Jasvinder Kaur Roshan Kumar Anjali Saxena Ankita Dua Charu Tripathi Chandni Talwar Charu Dogra Rawat Harpreet Kaur Helianthous Verma Jaspreet Kaur Mansi Verma Mona Singh Princy Hira Nirjara Singhvi Nitish Kumar Mahato Om Prakash Jaya Malhotra Rup Lal Vipin Chandra Kalia 《Indian journal of microbiology》2021,61(4):397
113.
Ali C Akyildiz Lambert Speelman Harald van Brummelen Miguel A Gutiérrez Renu Virmani Aad van der Lugt Anton FW van der Steen Jolanda J Wentzel Frank JH Gijsen 《Biomedical engineering online》2011,10(1):25
Background
Rupture of the cap of a vulnerable plaque present in a coronary vessel may cause myocardial infarction and death. Cap rupture occurs when the peak cap stress exceeds the cap strength. The mechanical stress within a cap depends on the plaque morphology and the material characteristics of the plaque components. A parametric study was conducted to assess the effect of intima stiffness and plaque morphology on peak cap stress. 相似文献114.
Renu Nandakumar Christophe Espirito Santo Nandakumar Madayiputhiya Gregor Grass 《Biometals》2011,24(3):429-444
Metallic copper surfaces have strong antimicrobial properties and kill bacteria, such as Escherichia coli, within minutes in a process called contact killing. These bacteria are exposed to acute copper stress under dry conditions
which is different from chronic copper stress in growing liquid cultures. Currently, the physiological changes of E. coli during the acute contact killing process are largely unknown. Here, a label-free, quantitative proteomic approach was employed
to identify the differential proteome profiles of E. coli cells after sub-lethal and lethal exposure to dry metallic copper. Of the 509 proteins identified, 110 proteins were differentially
expressed after sub-lethal exposure, whereas 136 proteins had significant differences in their abundance levels after lethal
exposure to copper compared to unexposed cells. A total of 210 proteins were identified only in copper-responsive proteomes.
Copper surface stress coincided with increased abundance of proteins involved in secondary metabolite biosynthesis, transport
and catabolism, including efflux proteins and multidrug resistance proteins. Proteins involved in translation, ribosomal structure
and biogenesis functions were down-regulated after contact to metallic copper. The set of changes invoked by copper surface-exposure
was diverse without a clear connection to copper ion stress but was different from that caused by exposure to stainless steel.
Oxidative posttranslational modifications of proteins were observed in cells exposed to copper but also from stainless steel
surfaces. However, proteins from copper stressed cells exhibited a higher degree of oxidative proline and threonine modifications. 相似文献
115.
Anne-Marie Estes Kathleen McAllen Michael S. Parker Renu Sah Trevor Sweatman Edwards A. Park Ambikaipakan Balasubramaniam Floyd R. Sallee Mary W. Walker Steven L. Parker 《Amino acids》2011,40(2):371-380
Treatment of CHO cells expressing human Y receptors (Y1, Y2 or Y4 subtype) with pertussis toxin results in a large decrease in functional receptors, with a preferential loss of heteropentameric
assemblies of receptor dimers and G-protein trimers. This occurs in parallel to inactivation of the nucleotide site of Gi
α subunits, with a half period of about 4 h. The loss could be mainly due to proteolysis at the level of recycling/perinuclear
endosomes, and of receptor completion in the ER, since it is reduced by co-treatment with ammonium chloride, an inhibitor
of particulate proteinases. Antagonists do not strongly decrease the heteropentameric fraction. These findings indicate that
the upkeep of Y receptor dimers in epithelial cell lines depends on the association of receptor oligomers with functional
Gi α subunits. This interaction could use the juxtamembrane helix 8 in the fourth intracellular domain, and could also be
supported by the C-terminal helix of the third intracellular loop, as outlined in the companion review (Parker et al., Amino
Acids, doi:, 2010). 相似文献
116.
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118.
Renu Kumari Deepak Kumar Samir K. Brahmachari Achal K. Srivastava Mohammed Faruq Mitali Mukerji 《Journal of genetics》2018,97(3):589-609
Cerebellar ataxias are a group of rare progressive neurodegenerative disorders with an average prevalence ranges from 4.8 to 13.8 in 100,000 individuals. The inherited disorders affect multiple members of the families, or a community that is endogamous or consanguineous. Presence of more than 3000 mutations in different genes with overlapping clinical symptoms, genetic anticipation and pleiotropy, as well as incomplete penetrance and variable expressivity due to modifiers pose challenges in genotype–phenotype correlation. Development of a diagnostic algorithm could reduce the time as well as cost in clinicogenetic diagnostics and also help in reducing the economic and social burden of the disease. In a unique research collaboration spanning over 20 years, we have been able to develop a paradigm for studying cerebellar ataxias in the Indian population which would also be relevant in other rare diseases. This has involved clinical and genetic analysis of thousands of families from diverse Indian populations. The extensive resource on ataxia has led to the development of a clinicogenetic algorithm for cost-effective screening of ataxia and a unique ataxia clinic in the tertiary referral centre in All India Institute of Medical Sciences. Utilizing a population polymorphism scanning approach, we have been able to dissect the mechanisms of repeat instability and expansion in many ataxias, and also identify founders, and trace the mutational histories in the Indian population. This provides information for genetic testing of at—risk as well as protected individuals and populations. To dissect uncharacterized cases which comprises more than 50% of the cases, we have explored the potential of next-generation sequencing technologies coupled with the extensive resource of baseline data generated in-house and other public domains. We have also developed a repository of patient-derived peripheral blood mononuclear cells, lymphoblastoid cell lines and neuronal lineages (derived from iPSCs) for ascribing functionality to novel genes/mutations. Through integrating these technologies, novel genes have been identified that has broadened the diagnostic panel, increased the diagnostic yield to over 75%, helped in ascribing pathogenicity to novel mutations and enabled understanding of disease mechanisms. It has also provided a platform for testing novel molecules for amelioration of pathophysiological phenotypes. This review through a perspective on CAs suggests a generic paradigm from diagnostics to therapeutic interventions for rare disorders in the context of heterogeneous Indian populations. 相似文献
119.
Poonam?Yadav Ravdeep?Kaur Mukesh?Kumar?Kanwar Renu?BhardwajEmail author Geetika?Sirhindi Leonard?Wijaya M.?N.?Alyemeni Parvaiz?AhmadEmail author 《Journal of Plant Growth Regulation》2018,37(2):575-590
The transition metal elements like copper act as double-edged sword for living cells. Cu, a redox active metal, is essential for various biological processes, but at higher concentrations it leads to toxicity by inducing production of reactive oxygen species (ROS). Thus, the objective of the present study was to investigate the effects of exogenously applied castasterone on oxidative stress markers and redox homeostasis managers in Brassica juncea plants subject to copper stress for 30 days. Copper-exposed plants showed accumulation of free radicals (H2O2 and superoxide anion) and lipid peroxidation. However, the exogenous treatment of seeds via the seed soaking method with different concentrations of castasterone reduced H2O2 production, superoxide anion radical content, and lipid peroxidation, thus indicating improved detoxification of ROS. Enzyme activity was increased by 19.19% for guaiacol peroxidase, 16.20% for superoxide dismutase, 35.74% for glutathione peroxidase, 27.58% for dehydroascorbate reductase, and 42.75% for ascorbate peroxidase, with castasterone pre-soaking under copper stress. The levels of non-enzymatic antioxidants were also increased with castasterone pre-treatment under copper stress. It may be concluded that castasterone treatment enhanced redox homeostasis managers in addition to increased levels of osmoprotectants. 相似文献
120.
Sandeep A. Walujkar Shreyas V. Kumbhare Nachiket P. Marathe Dhrati V. Patangia Parimal S. Lawate Renu S. Bharadwaj Yogesh S. Shouche 《World journal of microbiology & biotechnology》2018,34(6):76
Dysbiosis of intestinal microflora has been postulated in ulcerative colitis (UC), which is characterized by imbalance of mucosal tissue associated bacterial communities. However, the specific changes in mucosal microflora during different stages of UC are still unknown. The aim of the current study was to investigate the changes in mucosal tissue associated microbiota during acute exacerbations and remission stages of UC. The mucosal microbiota associated with colon biopsy of 12 patients suffering from UC (exacerbated stage) and the follow-up samples from the same patients (remission stage) as well as non-IBD subjects was studied using 16S rRNA gene-based sequencing and quantitative PCR. The total bacterial count in patients suffering from exacerbated phase of UC was observed to be two fold lower compared to that of the non-IBD subjects (p?=?0.0049, Wilcox on matched-pairs signed rank tests). Bacterial genera including Stenotrophomonas, Parabacteroides, Elizabethkingia, Pseudomonas, Micrococcus, Ochrobactrum and Achromobacter were significantly higher in abundance during exacerbated phase of UC as compared to remission phase. The alterations in bacterial diversity with an increase in the abnormal microbial communities signify the extent of dysbiosis in mucosal microbiota in patients suffering from UC. Our study helps in identifying the specific genera dominating the microbiota during the disease and thus lays a basis for further investigation of the possible role of these bacteria in pathogenesis of UC. 相似文献