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51.
Larval dispersal and movement patterns of coral reef fishes,and implications for marine reserve network design 下载免费PDF全文
Alison L. Green Aileen P. Maypa Glenn R. Almany Kevin L. Rhodes Rebecca Weeks Rene A. Abesamis Mary G. Gleason Peter J. Mumby Alan T. White 《Biological reviews of the Cambridge Philosophical Society》2015,90(4):1215-1247
Well‐designed and effectively managed networks of marine reserves can be effective tools for both fisheries management and biodiversity conservation. Connectivity, the demographic linking of local populations through the dispersal of individuals as larvae, juveniles or adults, is a key ecological factor to consider in marine reserve design, since it has important implications for the persistence of metapopulations and their recovery from disturbance. For marine reserves to protect biodiversity and enhance populations of species in fished areas, they must be able to sustain focal species (particularly fishery species) within their boundaries, and be spaced such that they can function as mutually replenishing networks whilst providing recruitment subsidies to fished areas. Thus the configuration (size, spacing and location) of individual reserves within a network should be informed by larval dispersal and movement patterns of the species for which protection is required. In the past, empirical data regarding larval dispersal and movement patterns of adults and juveniles of many tropical marine species have been unavailable or inaccessible to practitioners responsible for marine reserve design. Recent empirical studies using new technologies have also provided fresh insights into movement patterns of many species and redefined our understanding of connectivity among populations through larval dispersal. Our review of movement patterns of 34 families (210 species) of coral reef fishes demonstrates that movement patterns (home ranges, ontogenetic shifts and spawning migrations) vary among and within species, and are influenced by a range of factors (e.g. size, sex, behaviour, density, habitat characteristics, season, tide and time of day). Some species move <0.1–0.5 km (e.g. damselfishes, butterflyfishes and angelfishes), <0.5–3 km (e.g. most parrotfishes, goatfishes and surgeonfishes) or 3–10 km (e.g. large parrotfishes and wrasses), while others move tens to hundreds (e.g. some groupers, emperors, snappers and jacks) or thousands of kilometres (e.g. some sharks and tuna). Larval dispersal distances tend to be <5–15 km, and self‐recruitment is common. Synthesising this information allows us, for the first time, to provide species, specific advice on the size, spacing and location of marine reserves in tropical marine ecosystems to maximise benefits for conservation and fisheries management for a range of taxa. We recommend that: (i) marine reserves should be more than twice the size of the home range of focal species (in all directions), thus marine reserves of various sizes will be required depending on which species require protection, how far they move, and if other effective protection is in place outside reserves; (ii) reserve spacing should be <15 km, with smaller reserves spaced more closely; and (iii) marine reserves should include habitats that are critical to the life history of focal species (e.g. home ranges, nursery grounds, migration corridors and spawning aggregations), and be located to accommodate movement patterns among these. We also provide practical advice for practitioners on how to use this information to design, evaluate and monitor the effectiveness of marine reserve networks within broader ecological, socioeconomic and management contexts. 相似文献
52.
Thomas Saucède Huw Griffiths Camille Moreau Jennifer A. Jackson Chester Sands Rachel Downey Adam Reed Melanie Mackenzie Paul Geissler Katrin Linse 《ZooKeys》2015,(504):1-10
Information regarding the echinoids in this dataset is based on the Agassiz Trawl (AGT) and epibenthic sledge (EBS) samples collected during the British Antarctic Survey cruise JR275 on the RRS James Clark Ross in the austral summer 2012. A total of 56 (1 at the South Orkneys and 55 in the Eastern Weddell Sea) Agassiz Trawl and 18 (2 at the South Orkneys and 16 in the Eastern Weddell Sea) epibenthic sledge deployments were performed at depths ranging from ~280 to ~2060 m. This presents a unique collection for the Antarctic benthic biodiversity assessment of an important group of benthic invertebrates. In total 487 specimens belonging to six families, 15 genera, and 22 morphospecies were collected. The species richness per station varied between one and six. Total species richness represents 27% of the 82 echinoid species ever recorded in the Southern Ocean (David et al. 2005b, Pierrat et al. 2012, Saucède et al. 2014). The Cidaridae (sub-family Ctenocidarinae) and Schizasteridae are the two most speciose families in the dataset. They comprise seven and nine species respectively. This is illustrative of the overall pattern of echinoid diversity in the Southern Ocean where 65% of Antarctic species belong to the families Schizasteridae and Cidaridae (Pierrat et al. 2012). 相似文献
53.
Rene de Roland Lily-Arison 《Ostrich》2013,84(1-2):332-335
Lily-Arison, R.de R. 2000. Breeding biology of Frances's Sparrowhawk Accipiter francesii in a lowland rainforest of northeastem Madagascar. Ostrich 71 (1 & 2): 332–335. Frances's Sparrowhawks Accipiter fmncesii were studied during the breeding seasons 1994 and 1995 on Masoala Peninsula of northeastern Madagascar. Breeding coincides with the dry season, nest building in October, egg laying in November, hatching in December and fledging in January (beginning of the wet season). All but one of 10 pairs built new nests in 1995 and the mean distance of nest site from 1994 and 1995 nests was 105± 100 m (n = 6, ranging from 0 to 250m). Nests in primary forest were incubated for 79% of the observation time by females, 3% by males and eggs were unattended for 18% of the observation time. On a numerical basis the diet was composed of lizards (56%) and birds (23%), making up more than 79% of the identified prey items. A total of 33 eggs was laid in 13 nests (average clutch size 2.5). In 13 breeding attempts 29 (88%) hatched and all of those hatchlings fledged. Of the 14 My-documented breeding attempts 2.1 young fledged per breeding attempt and overall nesting success was 93%. L'Épervier de France Accipiter francesii a été étudié pendant la saison de reproduction en 1994 et 1995 dans la Presqu'Ile Masoala, au partie Nord-Est de Madagascar. La saison de reproduction de cette espkce coincide avec la saison sèche: la construction du nid en Octobre, la ponte en Novembre, I'élosion en Déembre et l'envol du poussin en Janvier (début de la saison pluviale). Tous, sauf une des 10 paires ont construit des nouveaw nids en 1995 et la distance moyenne de I'endroit du nid de I'anné 1994 et 1995 est de 105 + 100 m (n = 6, variant de 0 à 250m). Dans la for[ebar]t primaire, les oeufs ont éeté incubés par la femelle pendant 79% du temps d'observation, 3% par le mile et non incubés pendant 18% du temps. Les proies sont principalement composées de lkzards (56%) et oiseaux (23%), atteignant plus de 79% des proies identifiés. 33 oeufs ont été pondus dans 13 nids (en moyenne 2.5 oeufs par nid). Pour ces 13 nids, 29 oeufs (88%) ont été éclos et tous ces poussins (100%) sont capables de voler. Sur 14 nids observés, 2.1 poussins par nid s'envolent et le tam de succés est de 93%. 相似文献
54.
Smail SS Ayesh K Sierra-Montes JM Herrera RJ 《Insect biochemistry and molecular biology》2006,36(6):454-465
Five U6 small nuclear RNA (snRNA) isoforms were detected and characterized from the posterior silk gland (PSG) of the silk moth Bombyx mori (Nistari strain). Using the currently accepted U6 secondary structure model as a basis for comparison, the variants were analyzed for nucleotide differences across the sequence with a focus on known functional domains. Differences were observed primarily in single-stranded areas of which sixty percent were found in the highly conserved U4-U6 binding sites. In the Nistari strain, the U6A variant was found to be approximately four times more abundant as part of high molecular weight spliceosomal complexes when compared with U6A in the total unfractionated PSG cell lysate. Additionally, the European 703 B. mori strain total cell lysate U6 snRNA was analyzed and only the dominant U6A isoform initially identified in Nistari was found. Due to U6's essential role in pre-mRNA processing, variants may modulate assemblage of the catalytic core and in doing so potentially affect the rate of splicing. Phylogenetic analysis of the U6 snRNA sequences indicate an ancient divergence of U6 from the self-splicing group II intron module and a high degree of evolutionary conservation across species possibly due to functional constraints on the gene. Using in silico analysis, 35 full-length U6 variants were observed in the recently released Whole Genome Shotgun (WGS) database of the p50T strain. The consensus sequence of these U6 genes from p50T is identical to U6A identified in the Nistari strain. Furthermore p50T variant 1, which is represented in 14 genes, is equivalent to Nistari U6A. 相似文献
55.
Targeted deletion of integrin-linked kinase reveals a role in T-cell chemotaxis and survival 下载免费PDF全文
Liu E Sinha S Williams C Cyrille M Heller E Snapper SB Georgopoulos K St-Arnaud R Force T Dedhar S Gerszten RE 《Molecular and cellular biology》2005,25(24):11145-11155
Integrin-linked kinase (ILK) is a serine/threonine kinase that is important in cell-matrix interactions and cell signaling. To examine the role of ILK in leukocyte trafficking and survival, we generated T cell-specific ILK knockouts by breeding ILK(flox/flox) mice to transgenic mice expressing Cre recombinase under control of the Lck proximal promoter. Thymic T cells from Lck-Cre(+)/ILK(flox/flox) mice had a marked reduction (>95%) in ILK protein levels. Thymic cellularity was comparable in 3- to 4-week-old mice, but a threefold diminution of thymic T cells became evident by 6 to 8 weeks of age in the T cell-specific ILK knockout mice due to increased cell death of double-positive (DP) T cells. Analysis of peripheral T cells by quantitative PCR and by breeding Lck-Cre(+)/ILK(flox/flox) mice to a YFP-transgenic reporter strain demonstrated an approximate 20-fold enrichment of ILK-competent cells, suggesting these cells have a competitive advantage in trafficking to and/or survival in peripheral lymphatic organs. We explored mechanisms related to altered cell trafficking and survival that might explain the decreases in thymic cellularity and enrichment for ILK-competent cells in the spleen and lymph nodes. We observed a >50% reduction in chemotaxis of ILK-deficient T cells to the chemokines CXCL12 (stromal cell-derived factor [SDF]-1alpha) and CCL19 (macrophage inflammatory protein [MIP]-3beta), as well as enhanced apoptosis of ILK-deficient cells upon stress. Signaling studies in ILK-deficient T cells demonstrated diminished phosphorylation of Akt on the activating phosphorylation site, Ser 473, and a concordant decrease in Akt kinase activity following stimulation with the chemokine SDF-1. Rac1 activation was also markedly diminished in ILK-deficient T cells following chemokine stimulation. These data extend the role of ILK to immune-cell trafficking and survival via modulation of Akt- and Rac-dependent substrates, and have implications for cell recruitment in both homeostatic and pathological processes. 相似文献
56.
Natalia Presa Robin D. Clugston Susanne Lingrell Samuel E. Kelly Alfred H. Merrill Sayantan Jana Zamaneh Kassiri Antonio Gómez-Muñoz Dennis E. Vance Rene L. Jacobs Jelske N. van der Veen 《生物化学与生物物理学报:疾病的分子基础》2019,1865(1):14-25
Phosphatidylethanolamine N-methyltransferase (PEMT) converts phosphatidylethanolamine (PE) to phosphatidylcholine (PC), mainly in the liver. Pemt?/? mice are protected from high-fat diet (HFD)-induced obesity and insulin resistance, but develop severe non-alcoholic fatty liver disease (NAFLD) when fed a HFD, mostly due to impaired VLDL secretion. Oxidative stress is thought to be an essential factor in the progression from simple steatosis to steatohepatitis. Vitamin E is an antioxidant that has been clinically used to improve NAFLD pathology. Our aim was to determine whether supplementation of the diet with vitamin E could attenuate HFD-induced hepatic steatosis and its progression to NASH in Pemt?/? mice. Treatment with vitamin E (0.5?g/kg) for 3?weeks improved VLDL-TG secretion and normalized cholesterol metabolism, but failed to reduce hepatic TG content. Moreover, vitamin E treatment was able to reduce hepatic oxidative stress, inflammation and fibrosis. We also observed abnormal ceramide metabolism in Pemt?/? mice fed a HFD, with elevation of ceramides and other sphingolipids and higher expression of mRNAs for acid ceramidase (Asah1) and ceramide kinase (Cerk). Interestingly, vitamin E supplementation restored Asah1 and Cerk mRNA and sphingolipid levels. Together this study shows that vitamin E treatment efficiently prevented the progression from simple steatosis to steatohepatitis in mice lacking PEMT. 相似文献
57.
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59.
Yan Li Marie-Claire Héloir Xun Zhang Mareen Geissler Sophie Trouvelot Lucile Jacquens Marius Henkel Xin Su Xuewen Fang Qi Wang Marielle Adrian 《Molecular Plant Pathology》2019,20(8):1037-1050
Bacillus subtilis GLB191 (hereafter GLB191) is an efficient biological control agent against the biotrophic oomycete Plasmopara viticola, the causal agent of grapevine downy mildew. In this study, we show that GLB191 supernatant is also highly active against downy mildew and that the activity results from both direct effect against the pathogen and stimulation of the plant defences (induction of defence gene expression and callose production). High-performance thin-layer chromatography analysis revealed the presence of the cyclic lipopeptides fengycin and surfactin in the supernatant. Mutants affected in the production of fengycin and/or surfactin were thus obtained and allowed us to show that both surfactin and fengycin contribute to the double activity of GLB191 supernatant against downy mildew. Altogether, this study suggests that GLB191 supernatant could be used as a new biocontrol product against grapevine downy mildew. 相似文献
60.
Mansour S. Alturki Ngolui Rene Fuanta Madison A. Jarrard Judith V. Hobrath Douglas C. Goodwin Thankhoe A. Rantso Angela I. Calderón 《Bioorganic & medicinal chemistry letters》2018,28(4):802-808
Single dose high-throughput screening (HTS) followed by dose-response evaluations is a common strategy for the identification of initial hits for further development. Early identification and exclusion of false positives is a cost-saving and essential step in early drug discovery. One of the mechanisms of false positive compounds is the formation of aggregates in assays. This study evaluates the mechanism(s) of inhibition of a set of 14 compounds identified previously as actives in Mycobacterium tuberculosis (Mt) cell culture screening and in vitro actives in Mt shikimate kinase (MtSK) assay. Aggregation of hit compounds was characterized using multiple experimental methods, LC-MS, 1HNMR, dynamic light scattering (DLS), transmission electron microscopy (TEM), and visual inspection after centrifugation for orthogonal confirmation. Our results suggest that the investigated compounds containing oxadiazole-amide and aminobenzothiazole moieties are false positive hits and non-specific inhibitors of MtSK through aggregate formation. 相似文献