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111.
Renaud Prudent Céline F. SautelClaude Cochet 《Biochimica et Biophysica Acta - Proteins and Proteomics》2010,1804(3):493-498
Protein kinase CK2 is an unfavorable pronostic marker in several cancers and has consequently emerged as a relevant therapeutic target. Several classes of ATP-competitive inhibitors have been identified, showing variable effectiveness. The molecular architecture of this multisubunit enzyme could offer alternative strategies to develop small molecule inhibitors targeting different surfaces of the kinase. Polyoxometalates were identified as original CK2 inhibitors targeting key structural elements located outside the active site. In addition, the CK2 subunit interface represents an exosite distinct from the catalytic cavity that can be targeted by peptides or small molecules to achieve functional effects. 相似文献
112.
Philippe Bouché Pierre‐Cyril Renaud Philippe Lejeune Cédric Vermeulen Jean‐Marc Froment Alfred Bangara Okclefort Fiongai Antoine Abdoulaye Raymond Abakar Mike Fay 《African Journal of Ecology》2010,48(4):994-1003
The wildlife populations of Northern Central African Republic experienced precipitous declines during the 1970s and 1980s. While anecdotes coming out of the region indicate that the wildlife populations remain under serious threat, little is known about their status. An aerial sample count was carried out in the Northern Central African Republic at the end of the dry season in June 2005 and covered an 85,000 km2 complex landscape containing national parks, hunting reserves and community hunting areas. Results show a dramatic decline of wildlife since the previous survey in 1985. In 20 years, large mammals’ numbers decreased by 65%, probably because of poaching and diseases brought by illegal cattle transhumance. Elephant (Loxodonta africana) and Buffon kob (Kobus kob) populations showed the greatest decline (over 80% each), while buffalo (Syncerus caffer), roan antelope (Hippotragus equinus) and Giant Lord’s Derby Eland (Taurotragus derbianus) populations seem stable or increasing over these last 20 years. The analysis of the wildlife population distribution by status of the different types of protected areas (national parks, hunting areas) showed that individual encounter rates of elephant and buffalo were lower in national parks than in neighbouring hunting areas, while those for roan, giraffe (Giraffa camelopardalis) and Buffon kob were higher in the national parks. 相似文献
113.
Cecilia Ferndahl Nicklas Bonander Christel Logez Renaud Wagner Lena Gustafsson Christer Larsson Kristina Hedfalk Richard AJ Darby Roslyn M Bill 《Microbial cell factories》2010,9(1):47
Background
Recombinant protein production is universally employed as a solution to obtain the milligram to gram quantities of a given protein required for applications as diverse as structural genomics and biopharmaceutical manufacture. Yeast is a well-established recombinant host cell for these purposes. In this study we wanted to investigate whether our respiratory Saccharomyces cerevisiae strain, TM6*, could be used to enhance the productivity of recombinant proteins over that obtained from corresponding wild type, respiro-fermentative strains when cultured under the same laboratory conditions. 相似文献114.
115.
Distribution and expression of elicitin genes in the interspecific hybrid oomycete Phytophthora alni
Ioos R Panabières F Industri B Andrieux A Frey P 《Applied and environmental microbiology》2007,73(17):5587-5597
Phytophthora alni subsp. alni, P. alni subsp. multiformis, and P. alni subsp. uniformis are responsible for alder disease in Europe. Class I and II elicitin gene patterns of P. alni subsp. alni, P. alni subsp. multiformis, P. alni subsp. uniformis, and the phylogenetically close species P. cambivora and P. fragariae were studied through mRNA sequencing and 3' untranslated region (3'UTR)-specific PCRs and sequencing. The occurrence of multiple 3'UTR sequences in association with identical elicitin-encoding sequences in P. alni subsp. alni indicated duplication/recombination events. The mRNA pattern displayed by P. alni subsp. alni demonstrated that elicitin genes from all the parental genomes are actually expressed in this allopolyploid taxon. The complementary elicitin patterns resolved confirmed the possible involvement of P. alni subsp. multiformis and P. alni subsp. uniformis in the genesis of the hybrid species P. alni subsp. alni. The occurrence of multiple and common elicitin gene sequences throughout P. cambivora, P. fragariae, and P. alni sensu lato, not observed in other Phytophthora species, suggests that duplication of these genes occurred before the radiation of these species. 相似文献
116.
Development of High-Throughput Phenotyping of Metagenomic Clones from the Human Gut Microbiome for Modulation of Eukaryotic Cell Growth
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Karine Gloux Marion Leclerc Harout Iliozer Ren L'Haridon Chaysavanh Manichanh Grard Corthier Renaud Nalin Herv M. Blottire Joël Dor 《Applied microbiology》2007,73(11):3734-3737
Metagenomic libraries derived from human intestinal microbiota (20,725 clones) were screened for epithelial cell growth modulation. Modulatory clones belonging to the four phyla represented among the metagenomic libraries were identified (hit rate, 0.04 to 8.7% depending on the screening cutoff). Several candidate loci were identified by transposon mutagenesis and subcloning. 相似文献
117.
Renaud S Pugacheva EM Delgado MD Braunschweig R Abdullaev Z Loukinov D Benhattar J Lobanenkov V 《Nucleic acids research》2007,35(21):7372-7388
118.
The multixenobiotic resistance (MXR) system is a defence mechanism which protects living organisms by actively extruding xenobiotics from cells. Membrane transporters responsible for this phenotype can be induced by numerous compounds, but also by physical stressors such as temperature. In order to better understand the contribution of temperature in MXR expression, the MXR system was studied in the freshwater bivalve, Dreissena polymorpha. Results showed that the zebra mussel possesses a gene closely related to the ATP-binding cassette (ABC) subfamily B, which is expressed as a 120-kDa protein. Moreover, gill cells can transport the model substrate Rhodamine B in a verapamil-sensitive manner, which is a good indication of MXR protein activity such as P-glycoprotein (Pgp). When bivalves were slowly acclimated to temperatures ranging from 4 to 20 °C (maximum temperature variation, 2–3 °C/day), no significant change in MXR protein expression could be immunochemically measured. Furthermore, the MXR transport activity did not show significant variation in this temperature range. Conversely, incubation of zebra mussel specimens in 20 μM Dacthal dramatically enhanced both protein expression and transport activity, thus indicating that temperature is not a major determinant in zebra mussel gill Pgp expression. 相似文献
119.
120.
Tarek Ammar Wei Lin Amanda Higgins Lawrence J. Hayward Jean-Marc Renaud 《The Journal of general physiology》2015,146(6):509-525
The diaphragm muscle of hyperkalemic periodic paralysis (HyperKPP) patients and of the M1592V HyperKPP mouse model rarely suffers from the myotonic and paralytic symptoms that occur in limb muscles. Enigmatically, HyperKPP diaphragm expresses the mutant NaV1.4 channel and, more importantly, has an abnormally high Na+ influx similar to that in extensor digitorum longus (EDL) and soleus, two hindlimb muscles suffering from the robust HyperKPP abnormalities. The objective was to uncover the physiological mechanisms that render HyperKPP diaphragm asymptomatic. A first mechanism involves efficient maintenance of resting membrane polarization in HyperKPP diaphragm at various extracellular K+ concentrations compared with larger membrane depolarizations in HyperKPP EDL and soleus. The improved resting membrane potential (EM) results from significantly increased Na+ K+ pump electrogenic activity, and not from an increased protein content. Action potential amplitude was greater in HyperKPP diaphragm than in HyperKPP soleus and EDL, providing a second mechanism for the asymptomatic behavior of the HyperKPP diaphragm. One suggested mechanism for the greater action potential amplitude is lower intracellular Na+ concentration because of greater Na+ K+ pump activity, allowing better Na+ current during the action potential depolarization phase. Finally, HyperKPP diaphragm had a greater capacity to generate force at depolarized EM compared with wild-type diaphragm. Action potential amplitude was not different between wild-type and HyperKPP diaphragm. There was also no evidence for an increased activity of the Na+–Ca2+ exchanger working in the reverse mode in the HyperKPP diaphragm compared with the wild-type diaphragm. So, a third mechanism remains to be elucidated to fully understand how HyperKPP diaphragm generates more force compared with wild type. Although the mechanism for the greater force at depolarized resting EM remains to be determined, this study provides support for the modulation of the Na+ K+ pump as a component of therapy to alleviate weakness in HyperKPP. 相似文献