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991.
Renata Velozo Timbó Marcelo Hermes-Lima Luciano Paulino Silva Angela Mehta Maria Carolina Blassioli Moraes Débora Pires Paula 《PloS one》2014,9(10)
Plant defense response is an elaborate biochemical process shown to depend on the plant genetic background and on the biological stressor. This work evaluated the soybean biochemical foliar response to brown stink bug herbivory injury through an analysis of redox metabolism and proteomic 2DE profiles of susceptible (BRS Silvania RR) and resistant (IAC-100) varieties. The activity of lipoxygenase-3, guaiacol peroxidase, catalase and ascorbate peroxidase was monitored every 24 h up to 96 h. In the susceptible variety, injury caused an increase in the activities of lipoxygenase 3 and guaiacol peroxidase, no change in ascorbate peroxidase, and a decrease in catalase. In the resistant variety, injury did not cause an alteration of any of these enzymes. The proteomic profiles were evaluated after 24 h of injury and revealed to have a similar proportion (4–5%) of differential protein expression in both varieties. The differential proteins, identified by mass spectrometry, in the susceptible variety were related to general stress responses, to plant defense, and to fungal infections. However, in the resistant variety, the identified change in protein profile was related to Calvin cycle enzymes. While the susceptible variety showed adaptive changes in redox metabolism and expression of stress-responsive proteins, the resistant showed a defense response to circumvent the biological stressor. 相似文献
992.
993.
Antônio C. M. Queiroz Tatianne G. Marques Carla R. Ribas Tatiana G. Cornelissen Anselmo Nogueira Fernando A. Schmidt Rodrigo M. Feitosa Tathiana G. Sobrinho Yves Quinet Fabrício B. Baccaro Mônica A. Ulysséa André B. Vargas Maria Santina C. Morini Jorge L. P. Souza Lucas N. Paolucci Wesley Dáttilo Kleber Del-Claro Denise Lange Jean C. Santos Rogério R. Silva Renata B. F. Campos Emília Z. Albuquerque Thiago Izzo Ananza M. Rabello Ricardo R. C. Solar Stela A. Soares Karine S. Carvalho Aline B. Moraes Helena M. Torezan-Silingardi Larissa Nahas Iracenir Andrade dos Santos Cinthia B. Costa-Milanez Flávia Esteves Tiago Frizzo Ana Y. Harada Wesley DaRocha Eduardo Diehl-Fleig 《Biotropica》2023,55(1):29-39
Tropical studies traditionally describe insect diversity variation throughout the year. The temporally structured responses of insect assemblages to climate seasonality vary across ecosystems due to gradients of resource availability and limiting ecological factors. These idiosyncratic responses might be particularly true across the vast geographical range of the Brazilian territory, including various environments that harbor one of the most diverse ant faunas worldwide. This study addressed the relationship between ant diversity and climatic seasonality, performing a quantitative review of the published data on ant diversity collected in Brazil. We investigated the seasonality effect on ant abundance and richness described in the literature in 47 papers published between 2000 and 2018. These studies were developed mainly in the Atlantic Forest biome and collected ants with pitfall traps on the soil/litter stratum. We initially carried out a vote-counting procedure by comparing the number of significant results describing seasonal differences in the ant assemblage. We found that most papers described a similar pattern of ant abundance, richness, and species composition between seasons. However, when we performed a meta-analysis, we observed a clear pattern of higher ant abundance and richness in the wet/summer season compared with the dry/winter season. Our meta-analysis reveals that the ant diversity decreases in the dry season, strongly in the Cerrado biome. Additionally, we point out differences in the sampling effort across biomes, indicating the need for further investments in studies focused on temporal diversity patterns, including seasonal effects, on the insect assemblage in biomes less investigated so far. Abstract in Portuguese is available with online material. 相似文献
994.
Tatyana V. Votyakova Elena N. Bazhenova Renata A. Zvjagilskaya 《Journal of bioenergetics and biomembranes》1993,25(5):569-574
Spermine, spermidine, and magnesium ions modulate the kinetic parameters of the Ca2+ transport system ofEndomyces magnusii mitochondria. Mg2+ at concentrations up to 5 mM partially inhibits Ca2+ transport with a half-maximal inhibiting concentration of 0.5 mM. In the presence of 2 mM MgCl2, theS
0.5 value of the Ca2+ transport system increases from 220 to 490 µM, which indicates decreased affinity for the system. Spermine and spermidine exert an activating effect, having half-maximal concentrations of 12 and 50 µM, respectively. In the case of spermine, theS
0.5 value falls to 50–65 µM, which implies an increase in the transport system affinity for Ca2+. Both Mg2+ and spermine cause a decrease of the Hill coefficient, giving evidence for a smaller degree of cooperativity. Spermine and spermidine enable yeast mitochondria to remove Ca2+ from the media completely. In contrast, Mg2+ lowers the mitochondrial buffer capacity. When both Mg2+ and spermine are present in the medium, their effects on theS
0.5 value and the free extramitochondrial Ca2+ concentration are additive. The ability of spermine and Mg2+ to regulate yeast mitochondrial Ca2+ transport is discussed. 相似文献
995.
Bugiani Riccardo Govoni Paola Bottazzi Renata Giannico Paolo Montini Beatrice Pozza Mariano 《Aerobiologia》1995,11(1):41-46
Late blight caused byPhytophthora infestans is one of the most important diseases of tomato crops in Emilia Romagna. In total, 7–8 chemical sprays are applied per season to control the disease without taking into account the risk of infection. An integrated warning service for the prediction of tomato late blight was set up in order to define a more rational disease control strategy. As a part of the warning service, a number of sites monitoringPh. infestans airborne sporangia was set up for a timely disease prediction in the regional tomato growing areas. The results of 3 years monitoring ofPh. infestans sporangia are presented. They suggest that concentrations of airborne sporangia rapidly increase 1–2 weeks prior the blight onset in the field. 相似文献
996.
Renata Tavolaro Marcello Canonaco Maria Fosca Franzoni 《Cell and tissue research》1995,279(3):613-617
Neuroanatomic comparison of the binding capability of 2-[125I] iodomelatonin in the crested newt Triturus carnifex Laur. and the green frog Rana esculenta, using quantitative autoradiographic techniques, revealed a heterogeneous distribution pattern. The highest and relatively high binding activities were shown to occur in the optic tracts and in the suprachiasmatic area of the hypothalamus and the optic tectum, respectively, of both species. Low or no 2-[125I] iodomelatonin binding values were obtained in the preoptic nucleus, the tuberal hypothalamus, the medulla oblongata, the septum and the dorsal pallium. A differential binding pattern was observed in the amygdaloid nucleus pars lateralis, the striatum and the hindbrain of these amphibians. Indeed, notably high binding levels were shown to occur in the former two brain areas of the crested newt, whereas high levels were displayed in the latter brain region of the green frog. On the basis of elevated quantities of melatonin receptors in mesencephalic, hypothalamic and telencephalic sites, it seems plausible to ascribe some important sensory functions to this receptor system in both species. The remarkably different binding activities in the brain of the two amphibians could be correlated with the simpler cytoarchitectonic brain structure of urodeles and with species-specific variations. 相似文献
997.
The pattern of callose formation in meiotic cell walls and the order of megaspore degeneration and polarity during embryo sac development are investigated in F2-plants ofOe. hookeri ×suaveolens and the reciprocal cross. All investigated characters are variable between the ovules in the same ovary. Plants differ in the frequency of the types of callose pattern and polarity of the embryo sacs. In segregating progenies different combinations of both characters are found. The genetic basis of the polarity phenomena during the embryo sac development is discussed. In our material no correlation can be seen between the callose pattern in the surrounding wall of the meiotic cell and the development of polarity in the later stages. 相似文献
998.
Claudio Nicolini Pia Carlo Renata Finollo Fernando Vigo Barbara Cavazza Anna Ledda Enrica Ricci Giovanni Brambilla 《Cell biochemistry and biophysics》1984,6(3):183-196
Changes in the volume of rat liver nuclei have been monitored as a function of modifications in ionic environment (from 0 to 20 mM), temperature (from 4 to 37°C) and pH (from 1 to 8). An abrupt reduction of nuclear volume occurred with increasing ion concentration, this contraction being more pronounced with bivalent (either Ca2+ or Mg2+) than with monovalent (either Na+ or K+) cations. The lowering of pH produced a similar effect. Parallel changes in chromatin structure took place at the same time as phase-like transitions. Atomic absorption spectroscopy allowed determination of free and nuclei-bound ions, pointing to the presence of a sizeable number of free binding sites for chromatin-DNA even within intact nuclei. DNA-phosphate sites appear to be neutralized by ions strictly according to the size of the electric charge and polyelectrolyte theory. Partial digestion (by micrococcal nuclease) or simple breaks (by chemical carcinogens) of the chromatin-DNA fiber caused respectively elimination or reduction of the abrupt volume changes in the intact nuclei. The apparent role of chromatin structure versus nuclear matrix in determining the shape and volume of intact nuclei is briefly discussed. 相似文献
999.
Summary Many plasmids belonging to the F incompatibility groups contain more than one basic replicon. The chimeric plasmid pCG86 is an example of such a multireplicon plasmid. The two basic replicons of pCG86, RepFIIA/FIC and RepFIB have been cloned and re-ligated, the copy numbers of the clones have been determined, and the incompatibility behavior of plasmids containing the ligated replicons and the individual replicons has been studied. The bireplicon plasmids are not expected to be incompatible as recipients with monoreplicon RepFIB or RepFIIA/RepFIC plasmids, since when one replicon is challenged by an incoming replicon, the other should be able to handle the plasmid's replication. In our studies, we found that challenge with either monoreplicon plasmid resulted in incompatibility. This incompatibility was increased in bireplicon plasmids in which RepFIB was duplicated. We conclude that in the bireplicon plasmids, challenging the replication control of one replicon by an incompatible plasmid can interfere with the replication originating from the second replicon. 相似文献
1000.