加拿大自然保护区规划的启迪

为了更好地国际接轨,搞好自然保护区的管理工作,我们需要学习其他国家的先进经验,本文重点介绍加拿大自然保护区规划方面的典型经验,包括系统规划,综合管理规划,资源管理规划,服务规划,行动规划,以及各种规划之间的关系,并提出了对我们的启迪与可借鉴之处。     

氨肽酶N的表达及其与结石形成的关系(英文)

 为研究大鼠高胆固醇饮食时 ,肝脏氨肽酶N(APN)在实验结石形成中可能的结石发生作用 ,采用 1.2 %胆固醇饮食 4周 ,诱发新西兰兔胆囊结石形成 .根据兔APN基因cDNA序列设计引物 ,提取肝脏总RNA .利用RT PCR检测肝脏APNmRNA水平的变化 ,用组织化学方法观察肝脏毛细胆管膜上APN的表达 .观察新西兰兔胆囊结石形成过程中肝脏APN的mRNA水平的变化、APN表达及胆汁中APN活性、胆脂、总蛋白含量的变化 ,探讨APN在胆石形成中可能的作用 .经成石饲料饲养后 ,随着胆汁饱和度增加和APN活性加强 ,胆囊结石组肝脏APNmRNA水平较对照组明显增高 ,胆囊结石组胆汁中总胆固醇、CSI、总蛋白浓度及APN活性均明显高于对照组 ,且胆汁中APN活性与肝脏APN的表达及胆汁CSI增高呈正相关 .结果提示 ,当存在胆汁过饱和的情况下 ,APN很可能作为促成核因子在胆结石形成早期发挥重要作用     

Tropic1808基因的原核表达及其表达产物的生物活性

Tropic180 8基因是新近获得的一个鼠源性的cDNA .Tropic180 8基因开放阅读框架片段通过PCR方法从质粒中扩增后 ,重组入表达载体pET 2 1a中 ,转化大肠杆菌BL2 1(DE3 ) .用IPTG诱导目的蛋白的表达 ,SDS PAGE并凝胶图象分析确定目的蛋白表达水平占细菌总蛋白的 14%以上 .表达蛋白在N端融合有 16个氨基酸 ,将表达蛋白电转移至PVDF膜 .氨基酸序列分析表明 ,其N端第 17～ 2 5位氨基酸序列与Tropic180 8基因编码序列一致 .利用融合部分含T7·Tag ,通过亲和层析纯化表达蛋白 ,经Westernblot检测为目的蛋白 ,加入到无血清培养的新生SD大鼠背根神经节 (DRG)中 ,观察到表达蛋白对DRG具有促进存活和促进突起生长的作用 .     

膜翅目昆虫干标本的基因组DNA提取

提出了膜翅目昆虫干标本基因组DNA的提取方法,通过对实验结果和所测得的基因片段（28S D2 rDNA)的分析,表明该方法可行。     

膜翅目精子结构研究新进展

根据观察,膜翅目昆虫精子由顶体,精核,中部,尾部四个主要部分构成。顶体具顶体丝,中部具轴丝,中部与精核间具线粒体衍生物。本文同时对膜翅目,分别隶属12个科,10个总科中的2个亚目昆虫精子的顶体、精核、线粒体衍生物、轴丝等结构的研究状况进行概括,并应用精子结构特征对上述类群的系统发育状况进行讨论。     

α—硫辛酸对6—羟多巴胺诱导的PC12细胞凋亡的影响

PC12 cell line, a clonal cell line derived from a pheochromocytoma of rat adrenal medulla, was used as a model of dopaminergic neuron in vitro to study the effect of alpha-lipoic acid on the 6-OHDA induced apoptosis. The results from MTT method show that 6-OHDA decreased the cell survival rate obviously. Through TUNEL (TdT-mediated dUTP-biotion nick end labeling) and Flow cytometer (FCM) detection, we found that 6-OHDA triggered cell apoptosis and induced necrosis. It was confirmed by the different percentage of cell survival rate and apoptosis concluded from FCM and MTT. alpha-lipoic acid was used as antioxidant to protect the cell from 6-OHDA's injury. The result indicateed that alpha-lipoic acid can partly prevent apoptosis induced by 6-OHDA but fail to prevent necrosis since it can decrease the apoptotic cell from 20.09% to 3.09%, just as increased cell survival rate from 56.8% to 72.6% but can not reach the normal level showed by MTT assay. Biochemical approach showed the cell's antioxidant ability especial for SOD activity and GSH content increased after the treatment of alpha-lipoic acid. The data suggest that alpha-lipoic acid may protect PC12 cells from apoptosis induced by 6-OHDA through the antioxidant path.     

Calmodulin-binding protein BP-10, a probable new member of plant nonspecific lipid transfer protein superfamily.

CaMBP-10 is a novel plant endogenous calmodulin-binding protein with important physiological functions. The partial cDNA sequence of this protein was cloned using RT-PCR. The deduced peptide (designated PCBP10) is composed of 74 amino acid residues containing a basic amphiphilic alpha-helix typical for calmodulin-binding proteins. PCBP10 shows very high amino acid sequence homology with plant nonspecific lipid-transfer proteins (nsLTPs). Sequence analysis also reveals that PCBP10 has similar amino acid composition to plant nsLTPs, and seven of the eight conserved cysteine residues are found in PCBP10. Furthermore, the secondary structure features of PCBP10 are very similar to those of plant nsLTPs. In addition, there are striking resemblances between CaMBP-10 and plant nsLTPs in their biochemical and physical properties. Our results suggest that CaMBP-10 is a novel member of the plant and nsLTP gene family, and the Ca(2+)/CaM regulative system may also play roles in lipid metabolism, defense reactions, and the adaptation of plants to natural environment.     

Kinetics study of a selenium-containing ScFv catalytic antibody that mimics glutathione peroxidase.

The steady state kinetic study and some enzymic characterization of a selenium-containing scFv catalytic antibody (Se-scFv2F3) was carried out. A novel reaction formula of this abzyme-catalyzed reaction was proposed and a rate equation was gotten according to the formula. The constants in the equation were compared with Dalziel's parameters and the exact meanings of these constants were analyzed. The gotten kinetics parameters from the kinetics study of Se-scFv2F3 were analyzed and compared with that of native glutathione peroxidase.     

黄雀发声核团与部分听觉中枢内P物质的分布和性双态比较

用免疫组织化学方法研究P物质在雌雄黄雀发声控制核团和听觉中枢内的分布,结合计算机图像分析仪检测SP免疫阳性细胞和末梢的灰度值,并作雌雄比较。结果如下：1．在发声学习中枢嗅叶X区有大量的SP阳性神经末梢和一些神经细胞。2．在发声控制核团前脑高级发声中枢(HVc)、古纹状体栎核、发声学习中枢新纹状体巨细胞核和丘脑背内侧核外侧部内有许多的SP免疫阳性细胞。3．在发声控制中枢中脑背内侧核和延髓舌下神经核气管鸣管部、听觉中枢丘脑卵圆核的壳区、中脑背外侧核壳区及中脑丘间核等有密集的SP免疫阳性神经末梢和纤维分布;雄性发声中枢内SP的分布比雌性丰富,两者有显著的差异。结果表明：SP的分布在雌雄发声中枢之间存在显著的性双态;SP广泛分布于黄雀发声控制核团和部分听觉中枢内,提示SP可能在发声控制及听觉中枢内具有重要的生理功能。