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41.
Kinetics of Pseudomonas aeruginosa adhesion to 304 and 316-L stainless steel: role of cell surface hydrophobicity. 总被引:7,自引:4,他引:3 下载免费PDF全文
E Vanhaecke J P Remon M Moors F Raes D De Rudder A Van Peteghem 《Applied microbiology》1990,56(3):788-795
Fifteen different isolates of Pseudomonas aeruginosa were used to study the kinetics of adhesion to 304 and 316-L stainless steel. Stainless steel plates were incubated with approximately 1.5 X 10(7) CFU/ml in 0.01 M phosphate-buffered saline (pH 7.4). After the plates were rinsed with the buffer, the number of adhering bacteria was determined by a bioluminescence assay. Measurable adhesion, even to the electropolished surfaces, occurred within 30 s. Bacterial cell surface hydrophobicity, as determined by the bacterial adherence to hydrocarbons test and the contact angle measurement test, was the major parameter influencing the adhesion rate constant for the first 30 min of adhesion. A parabolic relationship between the CAM values and the logarithm of the adhesion rate constants (In k) was established. No correlation between either the salt aggregation or the improved salt aggregation values and the bacterial adhesion rate constants could be found. Since there was no significant correlation between the bacterial electrophoretic mobilities and the In k values, the bacterial cell surface charge seemed of minor importance in the process of adhesion of P. aeruginosa to 304 and 316-L stainless steel. 相似文献
42.
M.D.F. Santos H. Vermeersch J.P. Remon M. Schelkens P. De Backer R. Ducatelle F. Haesebrouck 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1996,682(2):301
A high-performance liquid chromatographic method for the analysis of doxycycline in turkey plasma samples using demeclocycline hydrochloride as the internal standard was developed, optimized and validated, A one-step extraction procedure and an isocratic HPLC method with UV detection were used. No interferences with endogenous compounds or with the anticoagulant were observed, Linear calibration curves (r2>0.99) were obtained in water and plasma between 0 and 600 μg ml−1. Good recoveries for doxycycline (>66%) and demeclocycline (>72%) were seen both in water and in plasma, The coefficient of variation was <9.86% for within-day reproducibility and <7.53% for the between-day reproducibility. The deviation between the mean value found and the true value was <14.5% (accuracy). The limit of detection was 0.1 μg ml−1 in plasma samples. A good stability of doxycycline was observed in water and in plasma samples after storage for six months at −20°C (recovery >91%). 相似文献
43.
Zaki Remon M. El-Dean Adel M. Kamal Radwan Shaban M. Ammar Mahmoud A. 《Russian Journal of Bioorganic Chemistry》2020,46(6):1293-1293
Russian Journal of Bioorganic Chemistry - An Erratum to this paper has been published: https://doi.org/10.1134/S1068162020060060 相似文献
44.
Three experiments were carried out to examine the consequences of concurrent infections with Ascaridia galli and Escherichia coli in chickens raised for table egg production. Characteristic pathological lesions including airsacculitis, peritonitis and/or polyserositis were seen in all groups infected with E. coli. Furthermore, a trend for increased mortality rates was observed in groups infected with both organisms which, however, could not be confirmed statistically. The mean worm burden was significantly lower in combined infection groups compared to groups infected only with A. galli. It was also shown that combined infections of E. coli and A. galli had an added significant negative impact on weight gain. 相似文献
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Delphine Depoix Sara Rute Marques David JP Ferguson Soraya Chaouch Thomas Duguet Robert E Sinden Philippe Grellier Linda Kohl 《Cellular microbiology》2020,22(3)
Sexual development is an essential phase in the Plasmodium life cycle, where male gametogenesis is an unusual and extraordinarily rapid process. It produces 8 haploid motile microgametes, from a microgametocyte within 15 minutes. Its unique achievement lies in linking the assembly of 8 axonemes in the cytoplasm to the three rounds of intranuclear genome replication, forming motile microgametes, which are expelled in a process called exflagellation. Surprisingly little is known about the actors involved in these processes. We are interested in kinesins, molecular motors that could play potential roles in male gametogenesis. We have undertaken a functional characterization in Plasmodium berghei of kinesin‐8B (PbKIN8B) expressed specifically in male gametocytes and gametes. By generating Pbkin8B‐gfp parasites, we show that PbKIN8B is specifically expressed during male gametogenesis and is associated with the axoneme. We created a ΔPbkin8B knockout cell line and analysed the consequences of the absence of PbKIN8B on male gametogenesis. We show that the ability to produce sexually differentiated gametocytes is not affected in ΔPbkin8B parasites and that the 3 rounds of genome replication occur normally. Nevertheless, the development to free motile microgametes is halted and the life cycle is interrupted in vivo. Ultrastructural analysis revealed that intranuclear mitoses are unaffected whereas cytoplasmic microtubules, although assembled in doublets and elongated, fail to assemble in the normal axonemal ‘9+2' structure and become motile. Absence of a functional axoneme prevented microgamete assembly and release from the microgametocyte, severely reducing infection of the mosquito vector. This is the first functional study of a kinesin involved in male gametogenesis. These results reveal a previously unknown role for PbKIN8B in male gametogenesis, providing new insights into Plasmodium flagellar formation. 相似文献
48.
Elissavet Nikolaou Ino Agrafioti Michael Stumpf Janet Quinn Ian Stansfield Alistair JP Brown 《BMC evolutionary biology》2009,9(1):44
Background
Microbes must sense environmental stresses, transduce these signals and mount protective responses to survive in hostile environments. In this study we have tested the hypothesis that fungal stress signalling pathways have evolved rapidly in a niche-specific fashion that is independent of phylogeny. To test this hypothesis we have compared the conservation of stress signalling molecules in diverse fungal species with their stress resistance. These fungi, which include ascomycetes, basidiomycetes and microsporidia, occupy highly divergent niches from saline environments to plant or mammalian hosts. 相似文献49.
Georgia Tsagkogeorga Xavier Turon Russell R Hopcroft Marie-Ka Tilak Tamar Feldstein Noa Shenkar Yossi Loya Dorothée Huchon Emmanuel JP Douzery Frédéric Delsuc 《BMC evolutionary biology》2009,9(1):187-16
Background
Tunicates have been recently revealed to be the closest living relatives of vertebrates. Yet, with more than 2500 described species, details of their evolutionary history are still obscure. From a molecular point of view, tunicate phylogenetic relationships have been mostly studied based on analyses of 18S rRNA sequences, which indicate several major clades at odds with the traditional class-level arrangements. Nonetheless, substantial uncertainty remains about the phylogenetic relationships and taxonomic status of key groups such as the Aplousobranchia, Appendicularia, and Thaliacea. 相似文献50.