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排序方式: 共有206条查询结果,搜索用时 281 毫秒
31.
Spassova Mariana I. Prins Theo W. Folkertsma Rolf T. Klein-Lankhorst René M. Hille Jacques Goldbach Rob W. Prins Marcel 《Molecular breeding : new strategies in plant improvement》2001,7(2):151-161
Tomato spotted wilt virus is an important threat to tomato production worldwide. A single dominant resistance gene locus, Sw5, originating from Lycopersicon peruvianum, has been identified and introgressed in cultivated tomato plants. Here we present the genomic organization of a 35 250 bp fragment of a BAC clone overlapping the Sw5 locus. Two highly homologous (95%) resistance gene candidates were identified within 40 kb of the CT220 marker. The genes, tentatively named Sw5-a and Sw5-b, encode proteins of 1245 and 1246 amino acids, respectively, and are members of the coiled-coil, nucleotide-binding-ARC, leucine-rich repeat group of resistance gene candidates. Promoter and terminator regions of the genes are also highly homologous. Both genes significantly resemble the tomato nematode and aphid resistance gene Mi and, to a lesser extent, Pseudomonas syringae resistance gene Prf. Transformation of Nicotiana tabacum cv. SR1 plants revealed that the Sw5-b gene, but not the Sw5-a gene, is necessary and sufficient for conferring resistance against tomato spotted wilt virus. 相似文献
32.
Jelle R. Dalenberg Swetlana Gutjar Gert J. ter Horst Kees de Graaf Remco J. Renken Gerry Jager 《PloS one》2014,9(12)
In the current study we show that non-verbal food-evoked emotion scores significantly improve food choice prediction over merely liking scores. Previous research has shown that liking measures correlate with choice. However, liking is no strong predictor for food choice in real life environments. Therefore, the focus within recent studies shifted towards using emotion-profiling methods that successfully can discriminate between products that are equally liked. However, it is unclear how well scores from emotion-profiling methods predict actual food choice and/or consumption. To test this, we proposed to decompose emotion scores into valence and arousal scores using Principal Component Analysis (PCA) and apply Multinomial Logit Models (MLM) to estimate food choice using liking, valence, and arousal as possible predictors. For this analysis, we used an existing data set comprised of liking and food-evoked emotions scores from 123 participants, who rated 7 unlabeled breakfast drinks. Liking scores were measured using a 100-mm visual analogue scale, while food-evoked emotions were measured using 2 existing emotion-profiling methods: a verbal and a non-verbal method (EsSense Profile and PrEmo, respectively). After 7 days, participants were asked to choose 1 breakfast drink from the experiment to consume during breakfast in a simulated restaurant environment. Cross validation showed that we were able to correctly predict individualized food choice (1 out of 7 products) for over 50% of the participants. This number increased to nearly 80% when looking at the top 2 candidates. Model comparisons showed that evoked emotions better predict food choice than perceived liking alone. However, the strongest predictive strength was achieved by the combination of evoked emotions and liking. Furthermore we showed that non-verbal food-evoked emotion scores more accurately predict food choice than verbal food-evoked emotions scores. 相似文献
33.
Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes 总被引:16,自引:0,他引:16
J. N. A. M. Rouppe van der Voort P. van Zandvoort H. J. van Eck R. T. Folkertsma R. C. B. Hutten J. Draaistra F. J. Gommers E. Jacobsen J. Helder J. Bakker 《Molecular & general genetics : MGG》1997,255(4):438-447
The allele specificity of AFLP markers was assessed in five relatively unrelated potato genotypes. To this end, two diploid
mapping populations of potato, F1SH × RH and F1AM × RH, were analysed using four and six AFLP primer combinations, respectively, recently applied to the analysis of the
genetically well characterized backcross population BC_C × E. The AFLP profiles of the five parents revealed 733 AFLP markers
and, when identical primer combinations were used, 131 comigrating AFLP markers were identified. After construction of five
parental maps, the genomic positions of these comigrating AFLP markers were compared and 117 markers (89%) which targeted
the same genomic region were assumed to be homologous. Of these putative homologues, 20 markers, each cloned from at least
two genotypes, were sequenced and 19 sets of amplification products were shown to be nearly identical. The number of AFLP
markers previously mapped in population BC_C × E ranged from three to eleven per chromosome, which allowed a reliable assessment
of chromosome numbers from individual linkage groups obtained in populations F1SH × RH and F1AM × RH. The high incidence of corresponding AFLP alleles was confirmed by using an additional set of five primer combinations.
The 733 AFLP markers localized provide a valuable reference collection for future mapping studies in potato. As a consequence
AFLP analysis may replace more laborious locus-specific marker techniques.
Received: 26 July 1996 / Accepted: 30 January 1997 相似文献
34.
Exopolysaccharides produced by Lactococcus lactis: from genetic engineering to improved rheological properties? 总被引:6,自引:0,他引:6
Michiel Kleerebezem Richard van Kranenburg Remco Tuinier Ingeborg C. Boels Pieternella Zoon Ellen Looijesteijn Jeroen Hugenholtz Willem M. de Vos 《Antonie van Leeuwenhoek》1999,76(1-4):357-365
Over the last years, important advances have been made in the study of the production of exopolysaccharides (EPS) by several lactic acid bacteria, including Lactococcus lactis. From different EPS-producing lactococcal strains the specific eps gene clusters have been characterised. They contain eps genes, which are involved in EPS repeating unit synthesis, export, polymerisation, and chain length determination. The function of the glycosyltransferase genes has been established and the availability of these genes opened the way to EPS engineering. In addition to the eps genes, biosynthesis of EPS requires a number of housekeeping genes that are involved in the metabolic pathways leading to the EPS-building blocks, the nucleotide sugars. The identification and characterisation of several of these housekeeping genes (galE, galU, rfbABCD) allows the design of metabolic engineering strategies that should lead to increased EPS production levels by L. lactis. Finally, model developme nt has been initiated in order to predict the physicochemical consequences of the addition of a EPS to a product. 相似文献
35.
36.
Ricardo Oliva Joe Win Sylvain Raffaele Laurence Boutemy Tolga O. Bozkurt Angela Chaparro‐Garcia Maria Eugenia Segretin Remco Stam Sebastian Schornack Liliana M. Cano Mireille van Damme Edgar Huitema Marco Thines Mark J. Banfield Sophien Kamoun 《Cellular microbiology》2010,12(6):705-715
Filamentous pathogens, such as plant pathogenic fungi and oomycetes, secrete an arsenal of effector molecules that modulate host innate immunity and enable parasitic infection. It is now well accepted that these effectors are key pathogenicity determinants that enable parasitic infection. In this review, we report on the most interesting features of a representative set of filamentous pathogen effectors and highlight recent findings. We also list and describe all the linear motifs reported to date in filamentous pathogen effector proteins. Some of these motifs appear to define domains that mediate translocation inside host cells. 相似文献
37.
Drabek K van Ham M Stepanova T Draegestein K van Horssen R Sayas CL Akhmanova A Ten Hagen T Smits R Fodde R Grosveld F Galjart N 《Current biology : CB》2006,16(22):2259-2264
In motile fibroblasts, stable microtubules (MTs) are oriented toward the leading edge of cells. How these polarized MT arrays are established and maintained, and the cellular processes they control, have been the subject of many investigations. Several MT "plus-end-tracking proteins," or +TIPs, have been proposed to regulate selective MT stabilization, including the CLASPs, a complex of CLIP-170, IQGAP1, activated Cdc42 or Rac1, a complex of APC, EB1, and mDia1, and the actin-MT crosslinking factor ACF7. By using mouse embryonic fibroblasts (MEFs) in a wound-healing assay, we show here that CLASP2 is required for the formation of a stable, polarized MT array but that CLIP-170 and an APC-EB1 interaction are not essential. Persistent motility is also hampered in CLASP2-deficient MEFs. We find that ACF7 regulates cortical CLASP localization in HeLa cells, indicating it acts upstream of CLASP2. Fluorescence-based approaches show that GFP-CLASP2 is immobilized in a bimodal manner in regions near cell edges. Our results suggest that the regional immobilization of CLASP2 allows MT stabilization and promotes directionally persistent motility in fibroblasts. 相似文献
38.
Komenda J Barker M Kuviková S de Vries R Mullineaux CW Tichy M Nixon PJ 《The Journal of biological chemistry》2006,281(2):1145-1151
The cyanobacterium Synechocystis sp. PCC 6803 contains four members of the FtsH protease family. One of these, FtsH (slr0228), has been implicated recently in the repair of photodamaged photosystem II (PSII) complexes. We have demonstrated here, using a combination of blue native PAGE, radiolabeling, and immunoblotting, that FtsH (slr0228) is required for selective replacement of the D1 reaction center subunit in both wild type PSII complexes and in PSII subcomplexes lacking the PSII chlorophyll a-binding subunit CP43. To test whether FtsH (slr0228) has a more general role in protein quality control in vivo, we have studied the synthesis and degradation of PSII subunits in wild type and in defined insertion and missense mutants incapable of proper assembly of the PSII holoenzyme. We discovered that, when the gene encoding FtsH (slr0228) was disrupted in these strains, the overall level of assembly intermediates and unassembled PSII proteins markedly increased. Pulse-chase experiments showed that this was due to reduced rates of degradation in vivo. Importantly, analysis of epitope-tagged and green fluorescent protein-tagged strains revealed that slr0228 was present in the thylakoid and not the cytoplasmic membrane. Overall, our results show that FtsH (slr0228) plays an important role in controlling the removal of PSII subunits from the thylakoid membrane and is not restricted to selective D1 turnover. 相似文献
39.
A Single Oncogenic Enhancer Rearrangement Causes Concomitant EVI1 and GATA2 Deregulation in Leukemia
Stefan Gröschel Mathijs A. Sanders Remco Hoogenboezem Elzo de Wit Britta A.M. Bouwman Claudia Erpelinck Vincent H.J. van der Velden Marije Havermans Roberto Avellino Kirsten van Lom Elwin J. Rombouts Mark van Duin Konstanze Döhner H. Berna Beverloo James E. Bradner Hartmut Döhner Bob Löwenberg Peter J.M. Valk Eric M.J. Bindels Wouter de Laat Ruud Delwel 《Cell》2014
40.