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The actions of neurotrophic factors i.e. basic fibroblast growth factor (bFGF, FGF-2) to neurons are related not only to neuronal
development and maintenance but also to synaptic plasticity regarding neurotransmission. We analyzed here the levels of FGF-2
immunoreactivity in the nucleus tractus solitarii (NTS) of Wistar Kyoto rats in response to alterations of neuronal activity
promoted by the stimulation of the baroreceptor reflex following an aortic coarctation-induced-hypertension. The FGF-2 immunoreactivity
(IR) was found in the cytoplasm of the neurons and in the nuclei of the glial cells in the NTS. A large number of NTS neurons
expressed FOS immunoreactivity 4 h after coarctation, as an indication of neuronal activity. Stereological methods showed
an increased number of FGF-2 immunoreactive (ir) neuronal profiles (90%) and glial profiles (149%) in the NTS of the 72 h
aortic coarctated rats. 1-week later, FGF-2 ir neurons were still increased (54%) but no change was found in the number of
FGF-2 ir glial profiles. The double immunoperoxidase method revealed that the majority of the FGF-2 ir glial cells was glial
fibrillary acidic protein (GFAP) positive astrocytes. GFAP immunohistochemistry showed an astroglial reaction at 72 h time-interval
(55%) but not 1 week after stimulation. The number of the cresyl violet positive neurons and OX42 ir profiles (marker of activated
microglia) in the NTS of coarctated rats were not different from control by 1 week and 1 month after the surgery, indicating
a lack of NTS injury in this period following coarctation hypertension. FGF-2 may be an important neurotrophic factor in areas
involved in the control of blood pressure. The increased FGF-2 IR in the NTS cells following neuronal stimulation may represent
trophic and plastic adaptive responses in this nucleus in an autocrine/paracrine fashion. 相似文献
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Lacerda Filho Armando Marsden Cavalcante Cecília Menelau Da Silva Andrey Batista Inácio Cicero Pinheiro de Lima-Neto Reginaldo Gonçalves de Andrade Melyna Chaves Leite Magalhães Oliane Maria Correia dos Santos Franz de Assis Graciano Neves Rejane Pereira 《Mycopathologia》2019,184(4):547-549
Mycopathologia - The article describes an atypical case of cat-transmitted ocular sporotrichosis due Sporothrix brasiliensis in a young patient. This report contributes significantly to knowledge... 相似文献
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Gleidson Benevides de Oliveira Hélio Noberto de Araújo Júnior Rejane dos Santos Sousa Ferdinando Vinícius Fernandes Bezerra Amilton Cesar dos Santos Carlos Eduardo Bezerra de Moura Alexandre Rodrigues Silva Hugo Alexandre de Oliveira Rocha Moacir Franco de Oliveira 《Journal of morphology》2019,280(8):1232-1245
The study investigated the gross and microscopic anatomy of the genital organs of 20 agoutis at different stages of the estrous cycle and four in the final trimester of pregnancy. Specimens were euthanized and their reproductive organs were fixed in a 4% paraformaldehyde or 2.5% glutaraldehyde solution and submitted to routine histological techniques for light and scanning electron microscopy. In the ovary, during the proestrus phase, we observed developing follicles and corpus luteum (CL) in regression; during estrus, there were Graafian follicles; during metestrus, there was a hemorrhagic corpus, whereas in diestrus, there was a mature CL. The uterus was partially double because the cervix was cranially septate but caudally, the septum disappeared, forming a single ostium that opened into the vagina. Changes occurred along the estrous cycle in the uterine and vaginal epithelia, that is, an increase in the uterine epithelium height accompanied by an increase of thickness of the vaginal epithelium during the follicular phase and a decrease of thickness of both epithelia during the luteal phase. The endometrial lining was composed of a simple cuboidal epithelium to simple columnar epithelium with basal nuclei. The vaginal mucosa consisted of epithelium that varied from nonkeratinized stratified squamous (luteal phase) to keratinized stratified squamous (follicular phase). The clitoris was external to the vagina. It presented two protruding lateral keratinized spicules and a centralized urethra, with no common parts between the urinary and genital tracts. Anatomical and histological changes were observed mainly in the cervix, vagina and spicules of the clitoris during the EC. 相似文献
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Maria Glria Teixeira Lacita Menezes Skalinski Enny S. Paixo Maria da Conceio N. Costa Florisneide Rodrigues Barreto Gubio Soares Campos Silvia Ines Sardi Rejane Hughes Carvalho Marcio Natividade Martha Itaparica Juarez Pereira Dias Soraya Castro Trindade Brbara Pereira Teixeira Vanessa Morato Eloisa Bahia Santana Cristina Borges Goes Neuza Santos de Jesus Silva Carlos Antonio de Souza Teles Santos Laura C. Rodrigues Jimmy Whitworth 《PLoS neglected tropical diseases》2021,15(4)
BackgroundChikungunya is an arbovirus, transmitted by Aedes mosquitoes, which emerged in the Americas in 2013 and spread rapidly to almost every country on this continent. In Brazil, where the first cases were detected in 2014, it currently has reached all regions of this country and more than 900,000 cases were reported. The clinical spectrum of chikungunya ranges from an acute self-limiting form to disabling chronic forms. The purpose of this study was to estimate the seroprevalence of chikungunya infection in a large Brazilian city and investigate the association between viral circulation and living condition.Methodology/principal findingsWe conducted a population-based ecological study in selected Sentinel Areas (SA) through household interviews and a serologic survey in 2016/2017. The sample was of 1,981 individuals randomly selected. The CHIKV seroprevalence was 22.1% (17.1 IgG, 2.3 IgM, and 1.4 IgG and IgM) and varied between SA from 2.0% to 70.5%. The seroprevalence was significantly lower in SA with high living conditions compared to SA with low living condition. There was a positive association between CHIKV seroprevalence and population density (r = 0.2389; p = 0.02033).Conclusions/significanceThe seroprevalence in this city was 2.6 times lower than the 57% observed in a study conducted in the epicentre of the CHIKV epidemic of this same urban centre. So, the herd immunity in this general population, after four years of circulation of this agent is relatively low. It indicates that CHIKV transmission may persist in that city, either in endemic form or in the form of a new epidemic, because the vector infestation is persistent. Besides, the significantly lower seroprevalences in SA of higher Living Condition suggest that beyond the surveillance of the disease, vector control and specific actions of basic sanitation, the reduction of the incidence of this infection also depends on the improvement of the general living conditions of the population. 相似文献
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Juan Carlos Florez Luciana Souto Mofatto Rejane do Livramento Freitas-Lopes Sávio Siqueira Ferreira Eunize Maciel Zambolim Marcelo Falsarella Carazzolle Laércio Zambolim Eveline Teixeira Caixeta 《Plant molecular biology》2017,93(6):607-621
Transgenic expression of the pepper Bs2 gene confers resistance to Xanthomonas campestris pv. vesicatoria (Xcv) pathogenic strains which contain the avrBs2 avirulence gene in susceptible pepper and tomato varieties. The avrBs2 gene is highly conserved among members of the Xanthomonas genus, and the avrBs2 of Xcv shares 96% homology with the avrBs2 of Xanthomonas citri subsp. citri (Xcc), the causal agent of citrus canker disease. A previous study showed that the transient expression of pepper Bs2 in lemon leaves reduced canker formation and induced plant defence mechanisms. In this work, the effect of the stable expression of Bs2 gene on citrus canker resistance was evaluated in transgenic plants of Citrus sinensis cv. Pineapple. Interestingly, Agrobacterium-mediated transformation of epicotyls was unsuccessful when a constitutive promoter (2× CaMV 35S) was used in the plasmid construction, but seven transgenic lines were obtained with a genetic construction harbouring Bs2 under the control of a pathogen-inducible promoter, from glutathione S-transferase gene from potato. A reduction of disease symptoms of up to 70% was observed in transgenic lines expressing Bs2 with respect to non-transformed control plants. This reduction was directly dependent on the Xcc avrBs2 gene since no effect was observed when a mutant strain of Xcc with a disruption in avrBs2 gene was used for inoculations. Additionally, a canker symptom reduction was correlated with levels of the Bs2 expression in transgenic plants, as assessed by real-time qPCR, and accompanied by the production of reactive oxygen species. These results indicate that the pepper Bs2 resistance gene is also functional in a family other than the Solanaceae, and could be considered for canker control. 相似文献
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Danielle Patrícia Cerqueira Macêdo Neiva Tinti de Oliveira Vanessa Karina Alves da Silva Aline Mary de Almeida Farias Reginaldo Gonçalves de Lima Neto Ana Botler Wilheim Patrícia Cariolano de Oliveira Nadyr Pedi Suanni Lemos de Andrade Rejane Pereira Neves 《Mycopathologia》2011,171(4):279-283
Trichosporon species are usually opportunistic pathogens. Here, we present a case of esophagitis caused by T. inkin in a 54-year-old woman with pulmonary cancer and severe neutropenia in whom the susceptibility profile of the isolate against
azoles and polyenes was verified. The patient was diagnosed with esophagitis grade I of Wilcox, presenting scattered whitish
plaques and exudates in upper two-thirds of the esophageal mucosa. Antifungal therapy involving oral fluconazole (150 mg/day
for 14 days) was ineffective. In vitro, the isolate showed no resistance to this azole and sensitivity to amphotericin B.
Since T. inkin is of growing importance as an agent of invasive infections in immunocompromised patients, we stress that the diagnosis of
esophagitis by this species should be followed by an assessment of the therapeutic sensitivity of the strain involved. 相似文献