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141.
Igor C. S. Cruz Miguel Loiola Tiago Albuquerque Rodrigo Reis José de Anchieta C. C. Nunes James D. Reimer Masaru Mizuyama Ruy K. P. Kikuchi Joel C. Creed 《PloS one》2015,10(1)
Consequences of reef phase shifts on fish communities remain poorly understood. Studies on the causes, effects and consequences of phase shifts on reef fish communities have only been considered for coral-to-macroalgae shifts. Therefore, there is a large information gap regarding the consequences of novel phase shifts and how these kinds of phase shifts impact on fish assemblages. This study aimed to compare the fish assemblages on reefs under normal conditions (relatively high cover of corals) to those which have shifted to a dominance of the zoantharian Palythoa cf. variabilis on coral reefs in Todos os Santos Bay (TSB), Brazilian eastern coast. We examined eight reefs, where we estimated cover of corals and P. cf. variabilis and coral reef fish richness, abundance and body size. Fish richness differed significantly between normal reefs (48 species) and phase-shift reefs (38 species), a 20% reduction in species. However there was no difference in fish abundance between normal and phase shift reefs. One fish species, Chaetodon striatus, was significantly less abundant on normal reefs. The differences in fish assemblages between different reef phases was due to differences in trophic groups of fish; on normal reefs carnivorous fishes were more abundant, while on phase shift reefs mobile invertivores dominated. 相似文献
142.
Matheus Bertanha Andrei Moroz Rodrigo G. Jaldin Regina A.M. Silva Jaqueline C. Rinaldi Márjorie A. Golim Sérgio L. Felisbino Maria A.C. Domingues Marcone L. Sobreira Patricia P. Reis Elenice Deffune 《Experimental cell research》2014
Clinical experience for peripheral arterial disease treatment shows poor results when synthetic grafts are used to approach infrapopliteal arterial segments. However, tissue engineering may be an option to yield surrogate biocompatible neovessels. Thus, biological decellularized scaffolds could provide natural tissue architecture to use in tissue engineering, when the absence of ideal autologous veins reduces surgical options. The goal of this study was to evaluate different chemical induced decellularization protocols of the inferior vena cava of rabbits. They were decellularized with Triton X100 (TX100), sodium dodecyl sulfate (SDS) or sodium deoxycholate (DS). Afterwards, we assessed the remaining extracellular matrix (ECM) integrity, residual toxicity and the biomechanical resistance of the scaffolds. Our results showed that TX100 was not effective to remove the cells, while protocols using SDS 1% for 2 h and DS 2% for 1 h, efficiently removed the cells and were better characterized. These scaffolds preserved the original organization of ECM. In addition, the residual toxicity assessment did not reveal statistically significant changes while decellularized scaffolds retained the equivalent biomechanical properties when compared with the control. Our results concluded that protocols using SDS and DS were effective at obtaining decellularized scaffolds, which may be useful for blood vessel tissue engineering. 相似文献
143.
We develop a maximum penalized-likelihood (MPL) method to estimate the fitnesses of amino acids and the distribution of selection coefficients (S = 2Ns) in protein-coding genes from phylogenetic data. This improves on a previous maximum-likelihood method. Various penalty functions are used to penalize extreme estimates of the fitnesses, thus correcting overfitting by the previous method. Using a combination of computer simulation and real data analysis, we evaluate the effect of the various penalties on the estimation of the fitnesses and the distribution of S. We show the new method regularizes the estimates of the fitnesses for small, relatively uninformative data sets, but it can still recover the large proportion of deleterious mutations when present in simulated data. Computer simulations indicate that as the number of taxa in the phylogeny or the level of sequence divergence increases, the distribution of S can be more accurately estimated. Furthermore, the strength of the penalty can be varied to study how informative a particular data set is about the distribution of S. We analyze three protein-coding genes (the chloroplast rubisco protein, mammal mitochondrial proteins, and an influenza virus polymerase) and show the new method recovers a large proportion of deleterious mutations in these data, even under strong penalties, confirming the distribution of S is bimodal in these real data. We recommend the use of the new MPL approach for the estimation of the distribution of S in species phylogenies of protein-coding genes. 相似文献
144.
Eliana M. Cancello Rogério R. Silva Alexandre Vasconcellos Yana T. Reis Luís M. Oliveira 《Biotropica》2014,46(4):441-450
In this study, we investigated the termites of the Brazilian Atlantic Forest, one of the most threatened biodiversity hotspots in the world, in regularly spaced sites from 7° S to 27° S latitude. To our knowledge, this is the only report of a latitudinal survey of termites at species level performed with a standardized sampling protocol. We evaluate termite diversity and abundance, and describe patterns of species composition based on feeding groups along the latitudinal gradient. We also describe the relative contribution of environmental variables to explain diversity patterns. Termite assemblages were investigated by standardized surveys at 15 Atlantic Forest sites, on six transects divided into five sections of 10 m², with 30 sections per site (or 300 m²/site), which were investigated by one trained person for one hour. Observed species richness and abundance were negatively correlated with latitude. The influence of latitude was explained mainly by variables related to temperature, precipitation and ambient energy (potential evapotranspiration). Our results also suggest that temperature exerts a greater constraint on Atlantic Forest termites than productivity, because ambient productivity increases with latitude in this forest but termite diversity decreases. Termite species richness in the Atlantic Forest showed a different pattern than those described for other organisms, increasing in diversity where the coastal‐forest strip narrows. Overall, our results indicate comparatively high termite species richness at northeastern sites and a significant impoverishment of termite assemblages in the southeastern and southern regions of the Atlantic Forest. 相似文献
145.
L.B. Silva A.P. Reis E.J.G. Pereira M.G.A. Oliveira R.N.C. Guedes 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2010,155(1):12-19
Serine proteinases from three strains of Sitophilus zeamais (Coleoptera: Curculionidae), one susceptible and two resistant to insecticides — one exhibiting fitness cost (resistant cost strain) and the other lacking it (resistant no-cost strain), were partially purified using an aprotinin–agarose affinity column providing purification factors ranging from 36.5 to 51.2%, with yields between 10 and 15% and activity between 529 and 875 µM/min/mg protein with the substrate N-α-benzoyl-l-Arg-p-nitroanilide (L-BApNA). SDS-PAGE of the purified fraction revealed a 56,000 Da molecular mass band in all strains and a 70,000 Da band more visible in the resistant no-cost strain. The purified proteinases from all strains were inhibited by phenylmethyl sulphonyl fluoride (PMSF), N-α-tosyl-l-lysine chloromethyl ketone (TLCK), aprotinin, benzamidine and soybean trypsin inhibitor (SBTI) characterizing them as trypsin-like serine proteinases. Trypsin-like proteinases from the resistant strains exhibited higher affinity for L-BApNA. The resistant no-cost strain exhibited Vmax-values 1.5- and 1.7-fold higher than the susceptible and resistance cost strains, respectively. A similar trend was also observed when using N-α-p-tosyl-L-Arg methyl ester (L-TAME) as substrate. These results provide support to the hypothesis that the enhanced serine proteinase activity may be playing a role in mitigating physiological costs associated with the maintenance of insecticide resistance mechanisms in some maize weevil strains. 相似文献
146.
The coastline of Sergipe state hosts the main Brazilian nesting sites of Lepidochelys olivacea (Eschscholtz, 1829). The second most abundant species of turtles in Sergipe is Caretta caretta (Linnaeus, 1758). Both sea turtle species, respectively known as olive ridley and loggerhead, are currently listed as endangered
by the International Union for the Conservation of Nature and Natural Resources. The genetic diversity of the Sergipe loggerhead
population (N = 51) was assayed by analyzing 627 bp from the control region of mitochondrial DNA in nesting females. Three haplotypes were
identified: CC-A4, CC-A24 and CC × LO. The last one was recorded for specimens considered hybrids because they represent L. olivacea’s mtDNA, but had the external morphology of C. caretta or of a mixture of both species. Based on the two types of hybrids, it was hypothesized that at least two hybridization events
had occurred: a more ancient hybridization event, accompanied by introgression (F2 or later backcrosses), and a recent one
(F1), both of which involving the same L. olivacea haplotype. The incidence of L. olivacea mitochondrial genome introgression into the C. caretta rookeries was only observed in Sergipe, which could be related to the large numbers of L. olivacea in this region and an overlap of reproduction periods and distribution areas of both species. This may also be associated
to global warming since it might alter the sex ratio of sea turtles, thus facilitating interspecific mating. Awareness of
gene flow between these species will significantly influence the development and implementation of adequate management strategies. 相似文献
147.
E. C. Reis L. S. Soares S. M. Vargas F. R. Santos R. J. Young K. A. Bjorndal A. B. Bolten G. Lôbo-Hajdu 《Conservation Genetics》2010,11(4):1467-1477
The loggerhead sea turtle, Caretta caretta, is the most common species of sea turtle nesting in Brazil and is listed as endangered by the IUCN. Our study characterizes
the genetic structure of loggerheads in Brazil based on mitochondrial DNA control region variability and presents a hypothesis
for the colonization of Brazilian rookeries. We analyzed 329 samples from Brazilian rookeries and an oceanic foraging ground,
and we compared our results with previously published data for other loggerhead populations. Brazilian rookeries had four
haplotypes, none of which have been reported for rookeries outside Brazil. Six haplotypes were found in the foraging aggregation.
The presence of the CC-A4 haplotype at all sampled sites and the low nucleotide diversity suggest a common origin for all
rookeries, with CC-A4 being the ancestral haplotype of the Brazilian populations. The occurrence of three haplotypes in the
foraging aggregation that are known only from rookeries outside of Brazil is consistent with the transoceanic migratory behavior
of loggerheads. Our results indicated that the colonization of Brazilian rookeries probably occurred from the southern USA
stock. This recent colonization most likely followed a north to south route along the Brazilian coastline, influenced by the
Brazilian warm current. Our results further suggest the existence of two genetic population units of loggerheads in Brazil
and corroborate natal homing behavior in loggerheads. 相似文献
148.
Augusto B. Reis Fabiano C. Araújo Virginia M. Pereira Adelina M. Dos Reis Robson A. Santos Fernando M. Reis 《Journal of molecular histology》2010,41(1):75-80
The presence of classical components of the renin-angiotensin system has been demonstrated in the male reproductive tract,
mainly in the testes and epididymis. The objective of this study was to verify the localization of angiotensin (Ang)-(1–7)
and its receptor Mas in human testis. The study included 12 men with previously proven fertility submitted to orchiectomy
for prostate cancer and 20 infertile men submitted to testicular biopsy for infertility work-up, comprising a subgroup with
obstructive azoospermia/normal spermatogenesis (n = 8) and another with non-obstructive azoospermia and severely impaired spermatogenesis (n = 12). Testicular tissue samples were processed by immunohistochemistry and real time polymerase chain reaction. Ang-(1–7)
was strongly expressed in the interstitial compartment, mainly in Leydig cells, with similar intensity in all groups evaluated.
The peptide was also detected in the seminiferous tubules, but with much less intensity compared to interstitial cells. The
receptor Mas was equally distributed between interstitial and tubular compartments and was found in all layers of the normal
seminiferous epithelium. However, neither Ang-(1–7) nor Mas were detected in the seminiferous tubules of samples with impaired
spermatogenesis. The testicular samples of infertile men with impaired spermatogenesis (non-obstructive azoospermia) expressed
Mas and ACE2 mRNA at lower concentrations (fold change = 0.06 and 0.04, respectively, P < 0.05) than samples with full spermatogenesis (obstructive azoospermia). This shows, for the first time, the immunolocalization
of Ang-(1–7) and its receptor Mas in testes of fertile and infertile men, and suggests that this system may be altered when
spermatogenesis is severely impaired. 相似文献
149.
150.
Márcio S. Araújo Eduardo G. Martins Leonardo D. Cruz Fernanda R. Fernandes Arício X. Linhares Sérgio F. Dos Reis Paulo R. Guimarães Jr 《Oikos》2010,119(1):81-88
Many generalist populations may actually be composed of relatively specialist individuals. This 'individual specialization' may have important ecological and evolutionary implications. Although this phenomenon has been documented in more than one hundred taxa, it is still unclear how individuals within a population actually partition resources. Here we applied several methods based on network theory to investigate the intrapopulation patterns of resource use in the gracile mouse opossum Gracilinanus microtarsus . We found evidence of significant individual specialization in this species and that the diets of specialists are nested within the diets of generalists. This novel pattern is consistent with a recently proposed model of optimal foraging and implies strong asymmetry in the interactions among individuals of a population. 相似文献