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51.
Spinal dorsal column afferent fiber composition in the pigeon: an electrophysiological investigation
Heinrich Reinke Reinhold Necker 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1992,171(3):397-403
Summary The spinal dorsal column of homing pigeons (Colomba livia) was investigated electrophysiologically by recording responses from individual afferent fibers at a high cervical level (segments C4-C5) to mechanical stimulation of wing skin and deep tissue. Of 157 afferent fibers 134 were cutaneous afferents. The remainder were afferents of deep receptors.Thirty of the cutaneous afferents were slowly adapting and 87 rapidly adapting (17 not identified). Rapidly adapting afferents were studied with regard to Pacinianlike characteristics (Herbst corpuscles in birds; vibration sensitive receptors). Of 43 rapidly adapting afferents 38 were classified as afferents of vibration sensitive Herbst corpuscles and 5 as non vibration sensitive rapidly adapting afferents; 44 afferents could not be studied sufficiently with regard to vibrational stimuli. The vibration sensitive Herbst corpuscle afferents had U-shaped vibrational tuning curves and responded best to vibration frequencies of 300 to 400 Hz. The 11 threshold for 300 Hz vibration ranged from 2 to 36 um. Herbst corpuscle afferents always showed strong phase coupling to the stimulus cycle.Afferents of deep receptors showed slowly adapting responses to firm pressure or movements of limbs and were classified as joint receptors. No muscle spindle afferents were encountered.Primary afferent fibers were identified in 89 cases (80 cutaneous and 9 deep), postsynaptic elements in 15 cases (11 cutaneous, 4 deep). Only slowly adapting responses were found in postsynaptic fibers.Abbreviations
CV
coefficient of variation
-
EI
entrainment index
-
INTH
interval histogram
-
PSTH
peristimulus time histogram
-
RA
rapidly adapting
-
SA
slowly adapting 相似文献
52.
Continuous monitoring of in vivo nitric oxide formation using EPR analysis in biliary flow. 总被引:2,自引:0,他引:2
A method to continuously monitor the nitric oxide (NO) level in anesthetized rats, using an in vivo trapping reaction of NO by iron-dithiocarbamate complex, is reported. Previously, we developed a method of monitoring NO in bile samples containing an NO complex excreted from the liver (Anal. Biochem. 243, 8-14, 1996). In the present study, we modified the method so that the bile flows directly through the EPR sample cell. Rats were injected with low doses of lipopolysaccharide (LPS) to induce NO formation and were later anesthetized. After cannulation, the bile duct was connected to the inlet of the EPR sample cell and the trapping agent iron complex of D-N-methylglucamine dithiocarbamate (MGD-Fe) was administered. The EPR signal level from NO complex of MGD-Fe in the flowing bile was continuously monitored. Using this method, immediate changes in in vivo NO level in rats were observed following administration of drugs that can affect NO formation. In addition, a continuous intravenous saline containing MGD-Fe made the EPR signal level stable and improved animal condition as well as survival time. Therefore, this method has two merits; (1) one can continuously monitor NO formation until it reaches the maximum level; (2) a rapid change in NO level after intervention can be followed. Using this method, we tested the effect of the substrate L-arginine and inhibitors for NO synthase activity and NO synthase induction. The sensitivity of the present method was tested by monitoring NO formation in rats after exposure to ionizing radiation. 相似文献
53.
Volker Gross Christian Reinke Frank Dette Roland Koch Dragos Vasilescu Thomas Penzel Ulrich Koehler 《Biomedizinische Technik》2007,52(1):73-76
Changes in normal lung sounds are an important sign of pathophysiological processes in the bronchial system and lung tissue. For the diagnosis of bronchial asthma, coughing and wheezing are important symptoms that indicate the existence of obstruction. In particular, nocturnal long-term acoustic monitoring and assessment make sense for qualitative and quantitative detection and documentation. Previous methods used for lung function diagnosis require active patient cooperation that is not possible during sleep. We developed a mobile device based on the CORSA standard that allows the recording of respiratory sounds throughout the night. To date, we have recorded 133 patients with different diagnoses (80 male, 53 female), of whom 38 were children. In 68 of the patients we could detect cough events and in 87 we detected wheezing. The recording method was tolerated by all participating adults and children. Our mobile system allows non-invasive and cooperation-independent nocturnal monitoring of acoustic symptoms in the domestic environment, especially at night, when most ailments occur. 相似文献
54.
Reinke A Anderson S McCaffery JM Yates J Aronova S Chu S Fairclough S Iverson C Wedaman KP Powers T 《The Journal of biological chemistry》2004,279(15):14752-14762
The Tor1p and Tor2p kinases, targets of the therapeutically important antibiotic rapamycin, function as components of two distinct protein complexes in yeast, termed TOR complex 1 (TORC1) and TORC2. TORC1 is responsible for a wide range of rapamycin-sensitive cellular activities and contains, in addition to Tor1p or Tor2p, two highly conserved proteins, Lst8p and Kog1p. By identifying proteins that co-purify with Tor1p, Tor2p, Lst8p, and Kog1p, we have characterized a comprehensive set of protein-protein interactions that define further the composition of TORC1 as well as TORC2. In particular, we have identified Tco89p (YPL180w) and Bit61p (YJL058c) as novel components of TORC1 and TORC2, respectively. Deletion of TOR1 or TCO89 results in two specific and distinct phenotypes, (i) rapamycin-hypersensitivity and (ii) decreased cellular integrity, both of which correlate with the presence of SSD1-d, an allele of SSD1 previously associated with defects in cellular integrity. Furthermore, we link Ssd1p to Tap42p, a component of the TOR pathway that is believed to act uniquely downstream of TORC1. Together, these results define a novel connection between TORC1 and Ssd1p-mediated maintenance of cellular integrity. 相似文献
55.
Danny C. Reinke 《Journal of phycology》1984,20(1):153-155
The structure of the frustule of auxospores, resting spores and vegetative cells of Chaetoceros muelleri Lemm. are described with LM and SEM. Vegetative frustules are relatively small and lightly silicified, are not united into filaments, and appear unornamented under LM and SEM. The setae are circular to subcircular in transverse section with spines and puncta arranged in a spiral pattern. The resting spore and auxospore frustules are more silicified than the vegetative frustules and appear unornamented under LM and SEM. The auxospores of C. muelleri were previously unknown. 相似文献
56.
Operons are found across multiple kingdoms and phyla, from prokaryotes to chordates. In the nematode Caenorhabditis elegans, the genome contains >1000 operons that compose ~15% of the protein-coding genes. However, determination of the force(s) promoting the origin and maintenance of operons in C. elegans has proved elusive. Compared to bacterial operons, genes within a C. elegans operon often show poor coexpression and only sometimes encode proteins with related functions. Using analysis of microarray and large-scale in situ hybridization data, we demonstrate that almost all operon-encoded genes are expressed in germline tissue. However, genes expressed during spermatogenesis are excluded from operons. Operons group together along chromosomes in local clusters that also contain monocistronic germline-expressed genes. Additionally, germline expression of genes in operons is largely independent of the molecular function of the encoded proteins. These analyses demonstrate that mechanisms governing germline gene expression influence operon origination and/or maintenance. Thus, gene expression in a specific tissue can have profound effects on the evolution of genome organization. 相似文献
57.
A Perfect Marker for Fragrance Genotyping in Rice 总被引:11,自引:2,他引:11
Louis?M.?T.?Bradbury Robert?J.?HenryEmail author Qingsheng?Jin Russell F.?Reinke Daniel L.E.?Waters 《Molecular breeding : new strategies in plant improvement》2005,16(4):279-283
Allele specific amplification (ASA) is a low-cost, robust technique that can be utilised to discriminate between alleles that
differ by SNP's, insertions or deletions, within a single PCR tube. Fragrance in rice, a recessive trait, has been shown to
be due to an eight bp deletion and three SNP's in a gene on chromosome 8 which encodes a putative betaine aldehyde dehydrogenase
2 (BAD2). Here we report a single tube ASA assay which allows discrimination between fragrant and non-fragrant rice varieties
and identifies homozygous fragrant, homozygous non-fragrant and heterozygous non-fragrant individuals in a population segregating
for fragrance. External primers generate a fragment of approximately 580 bp as a positive control for each sample. Internal
and corresponding external primers produce a 355 bp fragment from a non-fragrant allele and a 257 bp fragment from a fragrant
allele, allowing simple analysis on agarose gels. 相似文献
58.
59.
A targeted RNAi screen for genes involved in chromosome morphogenesis and nuclear organization in the Caenorhabditis elegans germline 总被引:3,自引:0,他引:3
We have implemented a functional genomics strategy to identify genes involved in chromosome morphogenesis and nuclear organization during meiotic prophase in the Caenorhabditis elegans germline. This approach took advantage of a gene-expression survey that used DNA microarray technology to identify genes preferentially expressed in the germline. We defined a subset of 192 germline-enriched genes whose expression profiles were similar to those of previously identified meiosis genes and designed a screen to identify genes for which inhibition by RNA interference (RNAi) elicited defects in function or development of the germline. We obtained strong germline phenotypes for 27% of the genes tested, indicating that this targeted approach greatly enriched for genes that function in the germline. In addition to genes involved in key meiotic prophase events, we identified genes involved in meiotic progression, germline proliferation, and chromosome organization and/or segregation during mitotic growth. 相似文献
60.
Summary This paper explores data compatibility issues arising from the assessment of remnant native vegetation condition using satellite remote sensing and field-based data. Space-borne passive remote sensing is increasingly used as a way of providing a total sample and synoptic overview of the spectral and spatial characteristics of native vegetation canopies at a regional scale. However, integrating field-collected data often not designed for integration with remotely sensed data can lead to data compatibility issues. Subsequent problems associated with the integration of unsuited datasets can contribute to data uncertainty and result in inconclusive findings. It is these types of problems (and potential solutions) that form the basis of this paper. In other words, how can field surveys be designed to support and improve compatibility with remotely sensed total surveys? Key criteria were identified for consideration when designing field-based surveys of native vegetation condition (and other similar applications) with the intent to incorporate remotely sensed data. The criteria include recommendations for the siting of plots, the need for reference location plots, the number of sample sites and plot size and distribution, within a study area. The difficulties associated with successfully integrating these data are illustrated using real examples taken from a study of the vegetation in the Little River Catchment, New South Wales, Australia. 相似文献