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21.
Zusammenfassung Zellfreie Extrakte aus Agaricus bisporus bilden Malat, Fumarat und Aspartat einerseits aus Pyruvat und CO2 in Gegenwart von Mn2+ und andererseits aus Phosphoenolpyruvat und CO2 in Gegenwart von Mg2+.Die Carboxylierung von Pyruvat wird durch ATP und NADPH2 deutlich gefördert, ist aber unabhängig von der Anwesenheit von CoA-Estern. Die Reaktion erfährt durch pCMB, Oxalat und Avidin eine Hemmung.Die Carboxylierung von Phosphoenolpyruvat wird durch ADP, nicht aber durch GDP und IDP gefördert.Aus den Ergebnissen wird geschlossen, daß bei der Carboxylierung von Pyruvat sowohl Pyruvatcarboxylase als auch Malatenzym wirksam sind, während für die Oxalacetatsynthese aus Phosphoenolpyruvat PEP-Carboxykinase verantwortlich ist.Die Bedeutung der drei Enzyme im Zusammenhang mit der Ernährung des Kulturchampignons aus dem natürlichen Substrat, mit der Glucogenese und der Steuerung des Citronensäurecyclus wird diskutiert.
Carboxylation reactions in Agaricus bisporus III. Pyruvate and phosphoenolpyruvate as CO2-acceptors
Summary Cell-free extracts from Agaricus bisporus catalyze the synthesis of malate, fumarate and aspartate from pyruvate and CO2 in the presence of Mn2+, and from phosphoenolpyruvate and CO2 with Mg2+ (partially replaceable by Mn2+).The carboxylation of pyruvate is highly stimulated by ATP and NADPH2, but is not affected by CoA-esters. The reaction is inhibited by pCMB, oxalate and avidin.The carboxylation of phosphoenolpyruvate is stimulated by ADP, but not by IDP and GDP.From cofactor-requirement and inhibitor studies it is concluded, that there are two enzymes, pyruvatecarboxylase and malic enzyme, which catalyze the carboxylation of pyruvate. Phosphoenolpyruvate carboxykinase is responsible for the CO2-fixation into oxaloacetate.The significance of these three enzymes is discussed in connection with the nutrition of the fungus from its natural growth substrate and with the regulation of glycogenesis and the citric acid cycle.相似文献
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Ultrasensitive bioluminescence immunoassays for the determination of peptides and proteins (illustrated with human urinary kallikrein, bradykinin and the determination of human urinary kallikrein antibody titres) have been developed. The usable ranges of the standard curves are from 5 pg to 5000 pg per litre. The relative intra-assay coefficients of variation of the tests were between 2% and 6%, and the inter-assay coefficients of variation between 4% and 12%. 相似文献
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City spore concentrations in the European Economic Community (EEC). VI. Poaceae (Grasses), 1982–1986
Frits Th. M. Spieksma Gennaro D'amato John Mullins Nicole Nolard Reinhard Wachter Eva R. Weeke 《Aerobiologia》1989,5(1):38-43
Summary Airborne grass-pollen concentrations in six cities in the EEC are compared, based on observations from five years, 1982–1986.
Results show that there are quantitative differences both between the monitoring stations and between the years. Very provisionally,
the average seasonal total of the European urban airborne grass-pollen concentration can be put at 4 to 5000 per m3 of air. Also qualitatively, regarding the seasonal fluctuations, there are differences between the stations, and between
the years. Generally, the results confirm that June is the most typical grass-pollen month in northwestern Europe, whereas
in mediterranean Europe May is the more prominent grass-pollen period. There seems to be little coherence between the starting
dates of the grass-pollen season even at relatively nearby stations in northwestern Europe, suggestive for a great influence
of the actual weather situation. 相似文献
26.
John F. Reinhard Jr. Emanuel J. Diliberto Jr. Alejandro J. Daniels 《Journal of neurochemistry》1989,52(4):1253-1259
Cultures of bovine adrenomedullary chromaffin cells accumulated 1-[methyl-3H]methyl-4-phenylpyridinium ([3H]MPP+) in a time- and concentration-dependent manner with an apparent Km of 0.7 microM and a Vmax of 3 pmol/min/10(6) cells. The uptake was sodium dependent and sensitive to inhibitors of the cell-surface catecholamine transporter. At low concentrations of MPP+, the subcellular distribution was identical to that of endogenous catecholamines in the catecholamine-containing chromaffin vesicles. However, at a higher concentration of MPP+, a larger proportion of the toxicant was recovered in the cytosolic fraction, with less in the chromaffin vesicle fractions. When cells were prelabeled with [3H]MPP+, at 1 and 300 microM, and then permeabilized with digitonin in the absence of Ca2+, there was a proportionally greater release of MPP+ from the cells labeled at the higher concentration of the toxicant. In the presence of Ca2+, cell permeabilization induced a time-dependent secretion of catecholamines and a parallel secretion of MPP+. Under these conditions, the secretion of endogenous catecholamines was unaffected by the presence of MPP+. When the permeabilization studies were carried out in the presence of tetrabenazine, a massive release of MPP+ was observed in the absence of Ca2+ and was not further increased by Ca2+. In intact cells prelabeled with 300 microM [3H]MPP+, the secretagogues nicotine and veratridine elicited a Ca2+ -dependent secretion of catecholamines and MPP+ from the cells in similar proportions to their cellular contents. Barium-induced release of both species was independent of external Ca2+.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
27.
Anaerobic degradation of toluene and xylene by aquifer microorganisms under sulfate-reducing conditions. 总被引:12,自引:5,他引:7 下载免费PDF全文
Toluene and the three isomers of xylene were completely mineralized to CO2 and biomass by aquifer-derived microorganisms under strictly anaerobic conditions. The source of the inoculum was gasoline-contaminated sediment from Seal Beach, Calif. Evidence confirming that sulfate was the terminal electron acceptor is presented. Benzene and ethylbenzene were not degraded under the experimental conditions used. Successive transfers of the mixed cultures that were enriched from aquifer sediments retained the ability to degrade toluene and xylenes. Greater than 90% of 14C-labeled toluene or 14C-labeled o-xylene was mineralized to 14CO2. The doubling time for the culture grown on toluene or m-xylene was about 20 days, and the cell yield was about 0.1 to 0.14 g of cells (dry weight) per g of substrate. The accumulation of sulfide in the cultures as a result of sulfate reduction appeared to inhibit degradation of aromatic hydrocarbons. 相似文献
28.
Initial reactions in anaerobic ethylbenzene oxidation by a denitrifying bacterium, strain EB1. 总被引:8,自引:3,他引:5 下载免费PDF全文
Initial reactions in anaerobic oxidation of ethylbenzene were investigated in a denitrifying bacterium, strain EB1. Cells of strain EB1 mineralized ethylbenzene to CO2 under denitrifying conditions, as demonstrated by conversion of 69% of [14C]ethylbenzene to 14CO2. In anaerobic suspensions of strain EB1 cells metabolizing ethylbenzene, the transient formation and consumption of 1-phenylethanol, acetophenone, and an as yet unidentified compound were observed. On the basis of growth experiments and spectroscopic data, the unknown compound is proposed to be benzoyl acetate. Cell suspension experiments using H2(18)O demonstrated that the hydroxyl group of the first product of anoxic ethylbenzene oxidation, 1-phenylethanol, is derived from water. A tentative pathway for anaerobic ethylbenzene mineralization by strain EB1 is proposed. 相似文献
29.
Peter Jacob Reinhard Meckbach Herwig G. Paretzke Ilya Likhtarev Ivan Los Lionella Kovgan Igor Komarikov 《Radiation and environmental biophysics》1994,33(3):251-267
Since the reactor accident of Chernobyl, cesium depth profiles and nuclide-specific kerma rates in air have been determined for various grassland sites in south Bavaria and in Ukraine. The sites are described by soil characteristics, annual precipitation, distance from release point, mode of deposition, and activity per unit area. The effects of surface roughness and migration of cesium into the soil on the kerma rate in air over grasslands was determined by two methods. The kerma rates in air obtained by the evaluations of in situ gamma-ray spectrometry results and of measured activity distributions in the soil showed only negligible differences for the observation period of 6 years after deposition. For the sites in Ukraine the kerma rate in air per activity per unit area was found to be systematically 40% higher than in Bavaria. The results from Bavaria on the attenuation of the kerma rate and a data set, including experiences from the weapons test fallout, are analytically approximated as a function of time up to 25 years after deposition. 相似文献
30.