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Metabolic Microenvironmental Control by Photosynthetic Biofilms under Changing Macroenvironmental Temperature and pH Conditions 下载免费PDF全文
Andrew Bissett Andreas Reimer Dirk de Beer Fumito Shiraishi Gernot Arp 《Applied microbiology》2008,74(20):6306-6312
Ex situ microelectrode experiments, using cyanobacterial biofilms from karst water creeks, were conducted under various pH, temperature, and constant-alkalinity conditions to investigate the effects of changing environmental parameters on cyanobacterial photosynthesis-induced calcification. Microenvironmental chemical conditions around calcifying sites were controlled by metabolic activity over a wide range of photosynthesis and respiration rates, with little influence from overlying water conditions. Regardless of overlying water pH levels (from 7.8 to 8.9), pH at the biofilm surface was approximately 9.4 in the light and 7.8 in the dark. The same trend was observed at various temperatures (4°C and 17°C). Biological processes control the calcium carbonate saturation state (Ω) in these and similar systems and are able to maintain Ω at approximately constant levels over relatively wide environmental fluctuations. Temperature did, however, have an effect on calcification rate. Calcium flux in this system is limited by its diffusion coefficient, resulting in a higher calcium flux (calcification and dissolution) at higher temperatures, despite the constant, biologically mediated pH. The ability of biological systems to mitigate the effects of environmental perturbation is an important factor that must be considered when attempting to predict the effects of increased atmospheric partial CO2 pressure on processes such as calcification and in interpreting microfossils in the fossil record. 相似文献
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Kevin C. Wakeman James D. Reimer Holger Jenke‐Kodama Brian S. Leander 《The Journal of eukaryotic microbiology》2014,61(4):343-353
Gregarines are a diverse group of apicomplexan parasites with a conspicuous extracellular feeding stage, called a “trophozoite”, that infects the intestines and other body cavities of invertebrate hosts. Although the morphology of trophozoites is very diverse in gregarines as a whole, high degrees of intraspecific variation combined with relatively low degrees of interspecific variation make the delimitation of different species based on trophozoite morphology observed with light microscopy difficult. The coupling of molecular phylogenetic data with comparative morphology has shed considerable light onto the boundaries and interrelationships of different gregarine species. In this study, we isolated a novel marine gregarine from the hepatic region of a Pacific representative of the hemichordate Glossobalanus minutus, and report the first ultrastructural and molecular data from any gregarine infecting this distinctive group of hosts. Molecular phylogenetic analyses of an SSU rDNA sequence derived from two single‐cell isolates of this marine gregarine demonstrated a strong and unexpected affiliation with a clade of terrestrial gregarines (e.g. Gregarina). This molecular phylogenetic data combined with a comparison of the morphological features in previous reports of gregarines collected from Atlantic representatives of G. minutus justified the establishment of a new binomial for the new isolate, namely Caliculium glossobalani n. gen. et sp. The molecular phylogenetic analyses demonstrated a clade of terrestrial gregarines associated with a sequence acquired from a marine species, which suggest that different groups of terrestrial/freshwater gregarines evolved independently from marine ancestors. 相似文献
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On site bioremediation of hydrocarbon-contaminated Arctic tundra soils in inoculated biopiles 总被引:15,自引:0,他引:15
W. Mohn C. Radziminski M.-C. Fortin K. Reimer 《Applied microbiology and biotechnology》2001,57(1-2):242-247
There is a need to develop technology to allow the remediation of soil in polar regions that have been contaminated by hydrocarbon fuel spills. Bioremediation is potentially useful for this purpose, but has not been well demonstrated in polar regions. We investigated biopiles for on-site bioremediation of soil contaminated with Arctic diesel fuel in two independent small-scale field experiments at different sites on the Arctic tundra. The results were highly consistent with one another. In biopiles at both sites, extensive hydrocarbon removal occurred after one summer. After 1 year in treatments with optimal conditions, total petroleum hydrocarbons were reduced from 196 to below 10 mg per kg of soil at one site, and from 2,109 to 195 mg per kg of soil at the other site. Addition of ammonium chloride and sodium phosphate greatly stimulated hydrocarbon removal and indicates that biodegradation was the primary mechanism by which this was achieved. Inoculation with cold-adapted, mixed microbial cultures further stimulated hydrocarbon removal during the summer immediately following inoculation. At one site, soil temperature was monitored during the summer season, and a clear plastic cover increased biopile soil temperature, measured as degree-day accumulation, by 30-49%. Our results show that on-site bioremediation of fuel-contaminated soil at Arctic tundra sites is feasible. 相似文献
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A new family, genus, and species of zooxanthellate macrocnemic zoanthid is described from Okinawa, Japan. The diminutive zoanthid N anozoanthus harenaceus sp. nov. occurs in sandy ‘pools’ upon hard substrates in coral reefs. The results of molecular phylogenetic analyses of mitochondrial 16S ribosomal DNA and cytochrome c oxidase subunit I suggests that Nanozoanthidae fam. nov. is genetically close to family Microzoanthidae and Isozoanthus sulcatus at the intrafamily–suborder level. The Nanozoanthidae fam. nov. –Microzoanthidae clade is clearly highly divergent from all other known zoanthid families and from the order Actiniaria at the suborder level or higher. These results demonstrate that much high‐level (e.g. above genus) diversity remains to be described within the order Zoantharia, and until such work is complete it will be difficult to completely understand their biodiversity. © 2013 The Linnean Society of London 相似文献
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Fujii, T. & Reimer, J. D. (2011). Phylogeny of the highly divergent zoanthid family Microzoanthidae (Anthozoa, Hexacorallia) from the Pacific. —Zoologica Scripta, 40, 418–431. In this study, one new family, one new genus and two new species of zoanthids from rubble zones spanning the temperate, subtropical and tropical Pacific Ocean are described. Two new species are described, Microzoanthus occultus sp. n. and Microzoanthus kagerou sp. n., both belonging to the new genus Microzoanthus and new family Microzoanthidae, and they can be clearly distinguished both morphologically and genetically from each other and other zoanthids by their very small size, reduced or absent stolon, habitat usually on the bottom side of rubble zone rocks, and divergent and distinct DNA (cytochrome oxidase subunit I, mitochondrial 16S ribosomal DNA, internal transcribed spacer region of ribosomal DNA) sequences. The phylogenetic analyses clearly show Microzoanthidae fam. n. to be genetically far different from all other hexacorallians at the order level, but the macrocnemic arrangement of mesenteries and other morphological characters (colonial specimens with narrow stolons, two rows of tentacles sand encrustation) clearly place these specimens within the order Zoantharia. This study demonstrates how it is highly likely the existence of many marine invertebrate taxa remains overlooked, and that widely distributed groups such as Microzoanthidae fam. n. remain to be discovered. 相似文献
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Rudolph Reimer Heike Helmbold Beata Szalay Christian Hagel Heinrich Hohenberg Wolfgang Deppert Wolfgang Bohn 《PloS one》2009,4(6)
Nestin is the characteristic intermediate filament (IF) protein of rapidly proliferating progenitor cells and regenerating tissue. Nestin copolymerizes with class III IF-proteins, mostly vimentin, into heteromeric filaments. Its expression is downregulated with differentiation. Here we show that a strong nestin expression in mouse embryo tissue coincides with a strong accumulation of the glucocorticoid receptor (GR), a key regulator of growth and differentiation in embryonic development. Microscopic studies on cultured cells show an association of GR with IFs composed of vimentin and nestin. Cells lacking nestin, but expressing vimentin, or cells expressing vimentin, but lacking nestin accumulate GR in the nucleus. Completing these networks with an exogenous nestin, respectively an exogenous vimentin restores cytoplasmic anchoring of GR to the IF system. Thus, heteromeric filaments provide the basis for anchoring of GR. The reaction pattern with phospho-GR specific antibodies and the presence of the chaperone HSC70 suggest that specifically the unliganded receptor is anchored to the IF system. Ligand addition releases GR from IFs and shifts the receptor into the nucleus. Suppression of nestin by specific shRNA abolishes anchoring of GR, induces its accumulation in the nucleus and provokes an irreversible G1/S cell cycle arrest. Suppression of GR prior to that of nestin prevents entry into the arrest. The data give evidence that nestin/vimentin specific anchoring modulates growth suppression by GR. We hypothesize that expression of nestin is a major determinant in suppression of anti-proliferative activity of GR in undifferentiated tissue and facilitates activation of this growth control in a precise tissue and differentiation dependent manner. 相似文献