全文获取类型
收费全文 | 3261篇 |
免费 | 265篇 |
国内免费 | 2篇 |
出版年
2023年 | 8篇 |
2022年 | 44篇 |
2021年 | 56篇 |
2020年 | 45篇 |
2019年 | 55篇 |
2018年 | 76篇 |
2017年 | 64篇 |
2016年 | 97篇 |
2015年 | 157篇 |
2014年 | 175篇 |
2013年 | 190篇 |
2012年 | 277篇 |
2011年 | 237篇 |
2010年 | 177篇 |
2009年 | 146篇 |
2008年 | 207篇 |
2007年 | 236篇 |
2006年 | 200篇 |
2005年 | 180篇 |
2004年 | 203篇 |
2003年 | 149篇 |
2002年 | 137篇 |
2001年 | 29篇 |
2000年 | 21篇 |
1999年 | 17篇 |
1998年 | 34篇 |
1997年 | 20篇 |
1996年 | 22篇 |
1995年 | 20篇 |
1994年 | 20篇 |
1993年 | 14篇 |
1992年 | 11篇 |
1991年 | 11篇 |
1990年 | 8篇 |
1989年 | 15篇 |
1987年 | 5篇 |
1986年 | 7篇 |
1985年 | 6篇 |
1984年 | 11篇 |
1983年 | 7篇 |
1982年 | 11篇 |
1981年 | 9篇 |
1980年 | 11篇 |
1978年 | 5篇 |
1977年 | 6篇 |
1976年 | 6篇 |
1975年 | 7篇 |
1974年 | 6篇 |
1966年 | 5篇 |
1931年 | 6篇 |
排序方式: 共有3528条查询结果,搜索用时 15 毫秒
41.
Boris Hinz 《Journal of biomechanics》2010,43(1):146-155
Tissues lose mechanical integrity when our body is injured. To rapidly restore mechanical stability a multitude of cell types can jump into action by acquiring a reparative phenotype—the myofibroblast. Here, I review the known biomechanics of myofibroblast differentiation and action and speculate on underlying mechanisms. Hallmarks of the myofibroblast are secretion of extracellular matrix, development of adhesion structures with the substrate, and formation of contractile bundles composed of actin and myosin. These cytoskeletal features not only enable the myofibroblast to remodel and contract the extracellular matrix but to adapt its activity to changes in the mechanical microenvironment. Rapid repair comes at the cost of tissue contracture due to the inability of the myofibroblast to regenerate tissue. If contracture and ECM remodeling become progressive and manifests as organ fibrosis, the outcome of myofibroblast activity will have more severe consequences than the initial damage. Whereas the pathological consequences of myofibroblast occurrence are of great interest for physicians, their mechano-responsive features render them attractive for physicists and bioengineers. Their well developed cytoskeleton and responsiveness to a plethora of cytokines fascinate cell biologists and biochemists. Finally, the question of the myofibroblast origin intrigues stem cell biologists and developmental biologists—what else can you ask from a truly interdisciplinary cell? 相似文献
42.
43.
Malliya Gounder Palanichamy Cai-Ling Zhang Bikash Mitra Boris Malyarchuk Miroslava Derenko Tapas Kumar Chaudhuri Ya-Ping Zhang 《BMC evolutionary biology》2010,10(1):304
Background
Tracing the genetic origin of central European farmer N1a lineages can provide a unique opportunity to assess the patterns of the farming technology spread into central Europe in the human prehistory. Here, we have chosen twelve N1a samples from modern populations which are most similar with the farmer N1a types and performed the complete mitochondrial DNA genome sequencing analysis. To assess the genetic and phylogeographic relationship, we performed a detailed survey of modern published N1a types from Eurasian and African populations. 相似文献44.
45.
Zusammenfassung Es wurde die Röntgenstrahleninaktivierung von Laktatdehydrogenase in Rattenleber und Invertase in Hefe untersucht. Die Inaktivierung war in den feuchten lebenden Zellen und in den getrockneten Zellen praktisch gleich, obwohl ein Beitrag der indirekten Strahlenwirkung in den feuchten Zellen zu erwarten war.Auszugsweise vorgetragen auf dem XI. Internationalen Kongreß für Radiologie, Rom, September 1965 相似文献
46.
47.
48.
Staphylococcus aureus Lpl protein triggers human host cell invasion via activation of Hsp90 receptor
Paula M. Tribelli Arif Luqman Minh‐Thu Nguyen Johannes Madlung Sook‐Ha Fan Boris Macek Peter Sass Katharina Bitschar Birgit Schittek Dorothee Kretschmer Friedrich Gtz 《Cellular microbiology》2020,22(1)
Staphylococcus aureus is a facultative intracellular pathogen. Recently, it has been shown that the protein part of the lipoprotein‐like lipoproteins (Lpls), encoded by the lpl cluster comprising of 10 lpls paralogue genes, increases pathogenicity, delays the G2/M phase transition, and also triggers host cell invasion. Here, we show that a recombinant Lpl1 protein without the lipid moiety binds directly to the isoforms of the human heat shock proteins Hsp90α and Hsp90ß. Synthetic peptides covering the Lpl1 sequence caused a twofold to fivefold increase of S. aureus invasion in HaCaT cells. Antibodies against Hsp90 decrease S. aureus invasion in HaCaT cells and in primary human keratinocytes. Additionally, inhibition of ATPase function of Hsp90 or silencing Hsp90α expression by siRNA also decreased the S. aureus invasion in HaCaT cells. Although the Hsp90ß is constitutively expressed, the Hsp90α isoform is heat‐inducible and appears to play a major role in Lpl1 interaction. Pre‐incubation of HaCaT cells at 39°C increased both the Hsp90α expression and S. aureus invasion. Lpl1‐Hsp90 interaction induces F‐actin formation, thus, triggering an endocytosis‐like internalisation. Here, we uncovered a new host cell invasion principle on the basis of Lpl‐Hsp90 interaction. 相似文献
49.
50.
Colin Tucker Anita Antoninka Natalie Day Boris Poff Sasha Reed 《Restoration Ecology》2020,28(Z2):S9-S16
Biocrusts' functional importance and vulnerability to disturbance have motivated consistent interest in biocrust restoration, as well as a recent increase in research to cultivate biocrusts in laboratory and greenhouse settings for use in ecological restoration. As part of a sustainable approach to developing biocrust restoration, we argue that a complementary step is to improve and accelerate methods for salvaging biocrusts that would otherwise be destroyed in a forthcoming disturbance. The increasing rate and scale of disturbance pressures in drylands where biocrusts flourish means that the supply of salvageable biocrust and demand for that material in restoration greatly exceed the present cultivable supply. In this article we describe the state of knowledge for biocrust salvage, present a simple set of steps for conducting a salvage harvest, discuss risks and benefits when considering using salvage, and suggest future research directions to facilitate scaling up biocrust restoration using salvaged material. A focus on the use of salvaged biocrust as a restoration source may prove an important step to improve ecological restoration in notoriously difficult to restore dryland ecosystems. 相似文献