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63.
Having historically been abundant throughout Europe, the house sparrow (Passer domesticus) has in recent decades suffered severe population declines in many urban and rural areas. The decline in rural environments
is believed to be caused by agricultural intensification, which has resulted in landscape simplification. We used giving-up
densities (GUDs) of house sparrows feeding in artificial food patches placed in farmlands of southern Sweden to determine
habitat quality during the breeding season at two different spatial scales: the landscape and the patch scale. At the landscape
scale, GUDs were lower on farms in homogeneous landscapes dominated by crop production compared to more heterogeneous landscapes
with mixed farming or animal husbandry. At the patch level, feeding patches with a higher predation risk (caused by fitting
a wall to the patch to obstruct vigilance) had higher GUDs. In addition, GUDs were positively related to population size,
which strongly implies that GUDs reflect habitat quality. However, the increase followed different patterns in homogeneous
and heterogeneous landscapes, indicating differing population limiting mechanisms in these two environments. We found no effect
of the interaction between patch type and landscape type, suggesting that predation risk was similar in both landscape types.
Thus, our study suggests that simplified landscapes constitute a poorer feeding environment for house sparrows during breeding,
that the population-regulating mechanisms in the landscapes differ, but that predation risk is the same across the landscape
types. 相似文献
64.
Wewer UM Mörgelin M Holck P Jacobsen J Lydolph MC Johnsen AH Kveiborg M Albrechtsen R 《The Journal of biological chemistry》2006,281(14):9418-9422
The ADAMs (a disintegrin and metalloprotease) comprise a family of multidomain proteins with metalloprotease, cell adhesion, and signaling activities. Human ADAM12, which is implicated in diseases such as cancer, is expressed in two splice forms, the transmembrane ADAM12-L and the shorter and soluble ADAM12-S. ADAM12 is synthesized as a zymogen with the prodomain keeping the metalloprotease inactive through a cysteine-switch mechanism. Maturation and activation of the protease involves the cleavage of the prodomain in the trans-Golgi or possibly at the cell surface by a furin-peptidase. The aim of the present study was to determine the fate of the prodomain following furin cleavage. Here we demonstrate that, following cleavage of the human ADAM12-S prodomain in the trans-Golgi by a furin-peptidase, the prodomain remains non-covalently associated with the mature molecule. Accordingly, both the 68-kDa mature form of ADAM12-S and the 25-kDa prodomain could be detected using domain-specific antisera in immunoprecipitation and Western blot analyses of human serum ADAM12 and purified recombinant human ADAM12. Using electron microscopy after negative staining we have furthermore obtained the first visualization of a full-length ADAM molecule, human ADAM12-S, and report that it appears to be a compact clover composed of four globular domains, one of which is the prodomain. Finally, our data demonstrate that the presence of the metalloprotease domain appears to be sufficient for the prodomain to remain associated with the mature ADAM12-S. Thus, we conclude that the prodomain of human ADAM12-S is an integral domain of the mature molecule and as such might have specific biological functions in the extracellular space. 相似文献
65.
Population characteristics predict responses in moose body mass to temporal variation in the environment 总被引:2,自引:0,他引:2
Herfindal I Saether BE Solberg EJ Andersen R Høgda KA 《The Journal of animal ecology》2006,75(5):1110-1118
1. A general problem in population ecology is to predict under which conditions stochastic variation in the environment has the stronger effect on ecological processes. By analysing temporal variation in a fitness-related trait, body mass, in 21 Norwegian moose Alces alces (L.) populations, we examined whether the influence of temporal variation in different environmental variables were related to different parameters that were assumed to reflect important characteristics of the fundamental niche space of the moose. 2. Body mass during autumn was positively related to early access to fresh vegetation in spring, and to variables reflecting slow phenological development (low June temperature, a long spring with a slow plant progression during spring). In contrast, variables related to food quantity and winter conditions had only a minor influence on temporal variation in body mass. 3. The magnitude of the effects of environmental variation on body mass was larger in populations with small mean body mass or living at higher densities than in populations with large-sized individuals or living at lower densities. 4. These results indicate that the strongest influence of environmental stochasticity on moose body mass occurs towards the borders of the fundamental niche space, and suggests that populations living under good environmental conditions are partly buffered against fluctuations in environmental conditions. 相似文献
66.
Clinical ethics committees have recently been established in nearly all Norwegian hospital trusts. One important task for these committees is clinical ethics consultations. This qualitative study explores significant barriers confronting the ethics committees in providing such consultation services. The interviews with the committees indicate that there is a substantial need for clinical ethics support services and, in general, the committee members expressed a great deal of enthusiasm for the committee work. They also reported, however, that tendencies to evade moral disagreement, conflict, and 'outsiders' are common in the hospitals. Sometimes even the committees comply with some of these tendencies. The committees agree that there is a need to improve their routines and procedures, clarify the committees' profile and field of responsibility, to make the committees well-known, to secure adequate operating conditions, and to develop organizational integration and support. Various strategies to meet these challenges on a local, regional or national level are also explored in this paper. 相似文献
67.
Marleen M. P. Cobben John D. C. Linnell Erling J. Solberg Reidar Andersen 《Ecological Research》2009,24(6):1197-1205
The survival rates and body masses of roe deer (Capreolus capreolus) were studied on the island of Storfosna in central Norway in relation to sex, age, season and year. There were no predators
on the island, and hunting was halted during the study period, resulting in a population increase from 10 to 40 individuals
per km2 during the period 1991–1994. A total of 352 individual roe deer were radio-monitored on a monthly basis. Survival rates were
analyzed using the MARK software. An age effect in survival was found separating fawns from yearlings and adults, and for
yearlings and adults we furthermore found a year effect. There was evidence for density dependence in body masses of fawns
and yearlings, but no density effect in survival rates. We found no sex effect in winter body mass, but a significant sex
effect in survival rates. We conclude that (1) increased population density can have an effect on body masses without causing
a change in survival rates (2) roe deer can maintain very high survival rates under favourable environmental conditions even
at very high population densities (3) male adults can reach equally high survival rates as females under favourable circumstances. 相似文献
68.
Maria H. Alanne Kati Pummi Anthony M. Heape Reidar Gr��nman Juha Peltonen Sirkku Peltonen 《The journal of histochemistry and cytochemistry》2009,57(6):523-529
Tight junctions (TJs) form physical barriers in various tissues and regulate paracellular transport of ions, water, and molecules. Myelinating Schwann cells form highly organized structures, including compact myelin, nodes of Ranvier, paranodal regions, Schmidt-Lanterman incisures, periaxonal cytoplasmic collars, and mesaxons. Autotypic TJs are formed in non-compacted myelin compartments between adjacent membrane lamellae of the same Schwann cell. Using indirect immunofluorescence and RT-PCR, we analyzed the expression of adherens junction (E-cadherin) and TJ [claudins, zonula occludens (ZO)-1, occludin] components in human peripheral nerve endoneurium, showing clear differences with published rodent profiles. Adult nerve paranodal regions contained E-cadherin, claudin-1, claudin-2, and ZO-1. Schmidt-Lanterman incisures contained E-cadherin, claudin-1, claudin-2, claudin-3, claudin-5, ZO-1, and occludin. Mesaxons contained E-cadherin, claudin-1, claudin-2, claudin-3, ZO-1, and occludin. None of the proteins studied were associated with nodal inter-Schwann cell junctions. Fetal nerve expression of claudin-1, claudin-3, ZO-1, and occludin was predominantly punctate, with a mesaxonal labeling pattern, but paranodal (ZO-1, claudin-3) and Schmidt-Lanterman incisure (claudins-1 and -3) expression profiles typical of compact myelin were visible by gestational week 37. The clear differences observed between human and published rodent nerve profiles emphasize the importance of human studies when translating the results of animal models to human diseases. (J Histochem Cytochem 57:523–529, 2009) 相似文献
69.
Background
Tumor-stroma reaction is associated with activation of fibroblasts. Nemosis is a novel type of fibroblast activation. It leads to an increased production of growth factors and proinflammatory and proteolytic proteins, while at the same time cytoskeletal proteins are degraded. Here we used paired normal skin fibroblasts and cancer-associated fibroblasts (CAF) and primary and recurrent oral squamous cell carcinoma (SCC) cells to study the nemosis response.Principal Findings
Fibroblast nemosis was analyzed by protein and gene expression and the paracrine regulation with colony formation assay. One of the normal fibroblast strains, FB-43, upregulated COX-2 in nemosis, but FB-74 cells did not. In contrast, CAF-74 spheroids expressed COX-2 but CAF-43 cells did not. Alpha-SMA protein was expressed in both CAF strains and in FB-74 cells, but not in FB-43 fibroblasts. Its mRNA levels were downregulated in nemosis, but the CAFs started to regain the expression. FSP1 mRNA was downregulated in normal fibroblasts and CAF-74 cells, but not in CAF-43 fibroblasts. Serine protease FAP was upregulated in all fibroblasts, more so in nemotic CAFs. VEGF, HGF/SF and FGF7 mRNA levels were upregulated to variable degree in nemosis. CAFs increased the colony formation of primary tumor cell lines UT-SCC-43A and UT-SCC-74A, but normal fibroblasts inhibited the anchorage-independent growth of recurrent UT-SCC-43B and UT-SCC-74B cells.Conclusions
Nemosis response, as observed by COX-2 and growth factor induction, and expression of CAF markers α-SMA, FSP1 and FAP, varies between fibroblast populations. The expression of CAF markers differs between normal fibroblasts and CAFs in nemosis. These results emphasize the heterogeneity of fibroblasts and the evolving tumor-promoting properties of CAFs. 相似文献70.
Odd Terje Sandlund Jon Museth Tor F. Næsje Sigurd Rognerud Randi Saksgård Trygve Hesthagen Reidar Borgstrøm 《Hydrobiologia》2010,650(1):27-41
Stable coexistence of Arctic charr and whitefish does occur in a number of native lake fish communities in Scandinavia. Even
so, whitefish introductions into Arctic charr lakes have resulted in serious decline and possibly local extinction of Arctic
charr. In this article, we analyze the habitat use and diet of the two species in five Norwegian lakes differing in basin
shape and environmental conditions. In two of the lakes, both species are native, and appear to live in a relatively stable
coexistence. Here, whitefish mainly occupy the littoral and upper pelagic zone, while Arctic charr live in the deeper habitats.
Diets are generally quite different in terms of the zooplankton species eaten. In the three other lakes, either whitefish
or both species have been introduced. In the shallowest lake, habitat segregation is similar to that seen in the pristine
lakes, although Arctic charr appears to be on the brink of extinction. In the remaining two lakes, however, Arctic charr dominates,
and occurs in higher numbers than whitefish in all the habitats. Our observations indicate that coexistence of the two species
in oligotrophic and relatively pristine lakes requires an extensive profundal zone to serve as a refugium for Arctic charr.
If the littoral zone is rendered inaccessible or unprofitable for whitefish due to dominance of a third competitor or predator,
or as a result of lake regulation, then Arctic charr may be the dominant species. 相似文献