全文获取类型
收费全文 | 1217篇 |
免费 | 114篇 |
出版年
2021年 | 14篇 |
2020年 | 8篇 |
2019年 | 10篇 |
2018年 | 20篇 |
2017年 | 10篇 |
2016年 | 15篇 |
2015年 | 36篇 |
2014年 | 33篇 |
2013年 | 51篇 |
2012年 | 92篇 |
2011年 | 56篇 |
2010年 | 42篇 |
2009年 | 38篇 |
2008年 | 53篇 |
2007年 | 37篇 |
2006年 | 41篇 |
2005年 | 38篇 |
2004年 | 39篇 |
2003年 | 43篇 |
2002年 | 28篇 |
2001年 | 21篇 |
2000年 | 26篇 |
1999年 | 19篇 |
1998年 | 17篇 |
1997年 | 17篇 |
1996年 | 16篇 |
1995年 | 13篇 |
1994年 | 9篇 |
1993年 | 15篇 |
1992年 | 16篇 |
1991年 | 20篇 |
1990年 | 16篇 |
1989年 | 18篇 |
1988年 | 16篇 |
1987年 | 12篇 |
1986年 | 17篇 |
1985年 | 16篇 |
1984年 | 23篇 |
1983年 | 10篇 |
1982年 | 16篇 |
1981年 | 16篇 |
1980年 | 11篇 |
1979年 | 13篇 |
1978年 | 13篇 |
1976年 | 9篇 |
1974年 | 9篇 |
1973年 | 9篇 |
1971年 | 9篇 |
1968年 | 7篇 |
1940年 | 8篇 |
排序方式: 共有1331条查询结果,搜索用时 468 毫秒
181.
182.
Rebecca Sydenham Yun Zheng Anja Riemens Adrian Tsang Justin Powlowski Reginald Storms 《Applied microbiology and biotechnology》2014,98(8):3613-3628
Endo-1,4-β-xylanases (EC 3.2.1.8) hydrolyze the 1,4-β-D-xylosidic linkages in xylans, the most abundant hemicellulose in plant cell walls. Xylanase enzymes have numerous industrial applications, including the manufacturing of animal feed, bread, juice and wine, pulp and paper, and biofuels. In this study, two glycosyl hydrolase family 10 members designated GtXyn10A and GtXyn10B and two glycosyl hydrolase family 11 members, OpXyn11A and CcXyn11C, were functionally expressed and subjected to biochemical characterization. The K M , V max, and k cat values of the four xylanases, determined using birchwood xylan, ranged from 0.27 to 1.1 mg/mL, 130 to 980 μmol/min/mg, and 109 to 344 s?1, respectively, where OpXyn11A gave the highest and GtXyn10B the lowest values for all three parameters. Substrate specificity studies and analysis of the products released during the degradation of xylo-oligosaccharides and three types of xylan revealed significant differences in catalytic properties, particularly between OpXyn11A and the other xylanases and between the family 10 and the family 11 xylanases. Molecular modeling suggests that the unique substrate specificity of OpXyn11A can be attributed to the presence of a serine rather that an asparagine or aspartate residue at the +1 substrate binding site. Additionally, all four xylanases exhibited biochemical characteristics of interest for various commercial applications. 相似文献
183.
184.
Dietary sodium intake for freshwater salmonids feeding in the wild (invertebrate diet) or in captivity (pellet diet) was calculated and compared with published branchial sodium influx values. Dietary sodium intake (mmol kg−1 per month) increases from winter minimum values of 5 and 30-40 to reach maximum values in summer of 175 and 240 for invertebrate and pellet diet, respectively. In summer, dietary sodium intake for fish feeding in the wild was of the same magnitude as branchial sodium influx. The implications of dietary sodium intake for sodium balance in freshwater fish are discussed. 相似文献
185.
Jeffrey M. Donlea Diogo Pimentel Clifford B. Talbot Anissa Kempf Jaison J. Omoto Volker Hartenstein Gero Miesenböck 《Neuron》2018,97(2):378-389.e4
186.
You stay,but I Hop: Host shifting near and far co‐dominated the evolution of Enchenopa treehoppers
下载免费PDF全文
![点击此处可从《Ecology and evolution》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yu‐Hsun Hsu Reginald B. Cocroft Robert L. Snyder Chung‐Ping Lin 《Ecology and evolution》2018,8(4):1954-1965
The importance and prevalence of phylogenetic tracking between hosts and dependent organisms caused by co‐evolution and shifting between closely related host species have been debated for decades. Most studies of phylogenetic tracking among phytophagous insects and their host plants have been limited to insects feeding on a narrow range of host species. However, narrow host ranges can confound phylogenetic tracking (phylogenetic tracking hypothesis) with host shifting between hosts of intermediate relationship (intermediate hypothesis). Here, we investigated the evolutionary history of the Enchenopa binotata complex of treehoppers. Each species in this complex has high host fidelity, but the entire complex uses hosts across eight plant orders. The phylogenies of E. binotata were reconstructed to evaluate whether (1) tracking host phylogeny; or (2) shifting between intermediately related host plants better explains the evolutionary history of E. binotata. Our results suggest that E. binotata primarily shifted between both distant and intermediate host plants regardless of host phylogeny and less frequently tracked the phylogeny of their hosts. These findings indicate that phytophagous insects with high host fidelity, such as E. binotata, are capable of adaptation not only to closely related host plants but also to novel hosts, likely with diverse phenology and defense mechanisms. 相似文献
187.
S S Grieshaber D H Lankenau T Talbot S Holland N S Petersen 《Cell motility and the cytoskeleton》2001,50(4):198-206
forked mutations affect bristle development in Drosophila pupae, resulting in short, thick, gnarled bristles in the adult. The forked proteins are components of 200-300-microm-long actin fiber bundles that are present transiently during pupal development [Petersen et al., 1994: Genetics 136:173-182]. These bundles are composed of segments of 3-10 microm long, and forked protein is localized along the actin fiber bundle segments and accumulates at the junctions connecting them longitudinally. In the forked mutants, f(36a) and f(hd), F-actin bundles are greatly reduced in number and size, and bundle segmentation is absent. The p-element, P[w(+), falter] contains a 5.3-kb fragment of the forked gene that encodes the 53-kD forked protein [Lankenau et al., 1996: Mol Cell Biol 16:3535-3544]. Expression of only the 53-kD forked protein is sufficient to rescue the actin bundle and bristle phenotypes of f(36a) and f(hd) mutant flies. The 5.3-kb forked sequence, although smaller than the 13-kb region previously shown to rescue forked mutants [Petersen et al., 1994: Genetics 136:173-182], does contain the core forked sequence that encodes actin binding and bundling domains in cultured mammalian cells [Grieshaber and Petersen, 1999: J Cell Sci 112:2203-2211]. These data show that the 53-kD forked protein is sufficient for normal bristle development and that the domains shown previously to be important for actin bundling in cell culture may be all that are required for normal actin bundle formation in developing Drosophila bristles. 相似文献
188.
In larval and early postlarval brown shrimp, Penaeus aztecus, portions of the branchial chamber are lined by a tissue which appears ultrastructurally to be modified for osmoregulation. The distribution of this tissue, the larval stages in which it occurs, and its appearance with the light and electron microscope are presented. The significance of the distribution and ultrastructure of this modified tissue is discussed. 相似文献
189.
190.
1. The rate of gluconeogenesis from alanine in the perfused rat liver is affected by the presence of other metabolizable substances, especially fatty acids, ornithine and ethanol. Gluconeogenesis is accelerated by oleate and by ornithine. When both oleate and ornithine were present the acceleration was greater than expected on the basis of mere additive effects. 2. Much NH(3) and some urea were formed from alanine when no ornithine was added. With ornithine almost all the nitrogen released from alanine appeared as urea. 3. Lactate was a major product of alanine metabolism. Addition of oleate, and especially of oleate plus ornithine, decreased lactate formation. 4. Ethanol had no major effect on gluconeogenesis from alanine when this was the sole added precursor. Gluconeogenesis was strongly inhibited (87%) when oleate was also added, but ethanol greatly accelerated gluconeogenesis when ornithine was added together with alanine. 5. In the absence of ethanol the alanine carbon and alanine nitrogen removed were essentially recovered in the form of glucose, lactate, pyruvate, NH(3) and urea. 6. In the presence of ethanol the balance of both alanine carbon and alanine nitrogen showed substantial deficits. These deficits were largely accounted for by the formation of aspartate and glutamine, the formation of which was increased two- to three-fold. 7. When alanine was replaced by lactate plus NH(4)Cl, ethanol also caused a major accumulation of amino acids, especially of aspartate and alanine. 8. Earlier apparently discrepant results on the effects of ethanol on gluconeogenesis from alanine are explained by the fact that under well defined conditions ethanol can inhibit, or accelerate, or be without major effect on the rate of gluconeogenesis. 9. It is pointed out that in the synthesis of urea through the ornithine cycle half of the nitrogen must be supplied in the form of asparate and half in the form of carbamoyl phosphate. The accumulation of aspartate and other amino acids suggests that ethanol interferes with the control mechanisms which regulate the stoicheiometric formation of aspartate and carbamoyl phosphate. 相似文献