全文获取类型
收费全文 | 209篇 |
免费 | 21篇 |
出版年
2022年 | 1篇 |
2021年 | 3篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 3篇 |
2016年 | 6篇 |
2015年 | 11篇 |
2014年 | 7篇 |
2013年 | 4篇 |
2012年 | 9篇 |
2011年 | 7篇 |
2010年 | 6篇 |
2009年 | 6篇 |
2008年 | 2篇 |
2007年 | 5篇 |
2006年 | 5篇 |
2005年 | 5篇 |
2004年 | 6篇 |
2002年 | 3篇 |
2001年 | 6篇 |
2000年 | 5篇 |
1999年 | 6篇 |
1998年 | 10篇 |
1997年 | 9篇 |
1996年 | 8篇 |
1995年 | 5篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 4篇 |
1990年 | 6篇 |
1989年 | 6篇 |
1988年 | 7篇 |
1987年 | 2篇 |
1986年 | 5篇 |
1985年 | 8篇 |
1984年 | 2篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 8篇 |
1976年 | 7篇 |
1975年 | 3篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1972年 | 3篇 |
1971年 | 2篇 |
1970年 | 1篇 |
排序方式: 共有230条查询结果,搜索用时 31 毫秒
151.
Abstract The McMurdo Dry Valley lakes, Antarctica, one of the Earth's southernmost ecosystems containing liquid water, harbor some of the most environmentally extreme (cold, nutrient-deprived) conditions on the planet. Lake Bonney has a permanent ice cover that supports a unique microbial habitat, provided by soil particles blown onto the lake surface from the surrounding, ice-free valley floor. During continuous sunlight summers (Nov.-Feb.), the dark soil particles are heated by solar radiation and melt their way into the ice matrix. Layers and patches of aggregates and liquid water are formed. Aggregates contain a complex cyanobacterial-bacterial community, concurrently conducting photosynthesis (CO2 fixation), nitrogen (N2) fixation, decomposition, and biogeochemical zonation needed to complete essential nutrient cycles. Aggregate-associated CO2- and N2-fixation rates were low and confined to liquid water (i.e., no detectable activities in the ice phase). CO2 fixation was mediated by cyanobacteria; both cyanobacteria and eubacteria appeared responsible for N2 fixation. CO2 fixation was stimulated primarily by nitrogen (NO3-), but also by phosphorus (PO43-). PO43- and iron (FeCl3 + EDTA) enrichment stimulated of N2 fixation. Microautoradiographic and physiological studies indicate a morphologically and metabolically diverse microbial community, exhibiting different cell-specific photosynthetic and heterotrophic activities. The microbial community is involved in physical (particle aggregation) and chemical (establishing redox gradients) modification of a nutrient- and organic matter-enriched microbial "oasis," embedded in the desertlike (i.e., nutrient depleted) lake ice cover. Aggregate-associated production and nutrient cycling represent microbial self-sustenance in a microenvironment supporting "life at the edge," as it is known on Earth. 相似文献
152.
Beuling EE van Dusschoten D Lens P van den Heuvel JC Van As H Ottengraf SP 《Biotechnology and bioengineering》1998,60(3):283-291
The mobility of water in intact biofilms was measured with pulsed field gradient nuclear magnetic resonance (PFG-NMR) and used to characterise their diffusive properties. The results obtained with several well-defined systems, viz. pure water, agar, and agar containing inert particles or active bacteria were compared to glucose diffusion coefficients measured with micro-electrodes and those calculated utilising theoretical diffusion models. A good correspondence was observed indicating that PFG-NMR should also enable the measurement of diffusion coefficients in heterogeneous biological systems. Diffusion coefficients of several types of natural biofilms were measured as well and these results were related to the physical biofilm characteristics. The values had a high accuracy and reflected the properties of a sample of ca. 100 biofilms, while non-uniformity or non-geometrical shapes did not negatively influence the results. The monitored PFG-NMR signal contains supplementary information on e.g. cell fraction or spatial organisation but quantitative analysis was not yet possible. Copyright 1998 John Wiley & Sons, Inc. 相似文献
153.
154.
Pseudomonads and achromobacters in the spoilage of irradiated haddock of different preirradiation quality 总被引:1,自引:1,他引:0
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The effect of initial quality of fish on postirradiation (100 krad) changes in the bacterial flora of haddock fillets during aerobic storage at 3 C has been investigated, with emphasis on the Pseudomonas and Achromobacter groups. The quality was related to the length of time the eviscerated fish had been stored in ice prior to filleting. Increased numbers of organisms, in particular Pseudomonas putrefaciens, were found initially on fillets cut from older fish. Pseudomonads were reduced by 2 to 3 log orders by irradiation, and achromobacters and gram-positive isolates predominated in the immediate postirradiation flora. Little difference could be detected in either types or relative proportions of organisms occurring during storage of unirradiated fish of different quality. Pseudomonads outgrew achromobacters and dominated the spoilage flora in all cases. After spoilage, however, the growth rate of pseudomonads declined markedly. In irradiated fish, achromobacters predominated throughout storage. In fish of better initial quality, bacterial numbers were 1 to 2 log orders higher at spoilage than in their unirradiated counterparts and in the poorer quality of irradiated samples. The increased number of organisms was accompanied by a radical change in the character of the predominant achromobacters. Pseudomonads were found to increase in numbers during storage of irradiated fish, in particular in poorer quality fish on which they were initially present in higher numbers. Detection of pseudomonads, even when present in high numbers, was found to be limited by the identification techniques normally used. 相似文献
155.
156.
157.
158.
JU Nnadi IN Dimelu SI Nwani JC Madu CI Atama GN Attamah 《African Journal of Aquatic Science》2018,43(1):27-34
The current study investigated the effects of termite insecticide, Termex® (imidacloprid 35.50% SC), on biometric variations and oxidative stress biomarkers in Clarias gariepinus. Fish were exposed to 4.00 and 6.00 µg l–1 sublethal Termex® concentrations in 2017. The gill and liver tissues were sampled on days 7, 14, 21 and 28 and the results indicated that hepatosomatic index (HSI) decreased significantly when compared with the control on days 14, 21 and 28. The condition factor (CF) and viscera-somatic index (VSI) also decreased during the study period. The decrease was greater at 6.00 µg l–1 Termex® concentration on days 21 and 28 for CF and days 14 to 28 for VSI, respectively. The lipid peroxidation (LPO) in both tissues was highest in the 6.00 µg l?1 Termex® and increased with the duration. There was significant decrease (p < 0.05) in superoxide dismutase and glutathione peroxidase values, but significant increase in catalase activity in both tissues. The values of glutathione reductase in both tissues were comparable to the control, except on days 21 and 28 in the liver. There was negative correlation between the LPO in tissues and the HSI, CF and VSI values. The use of Termex® in the environment should be monitored to safeguard the health of aquatic organisms. 相似文献
159.
160.