全文获取类型
收费全文 | 971篇 |
免费 | 47篇 |
出版年
2023年 | 12篇 |
2022年 | 11篇 |
2021年 | 31篇 |
2020年 | 21篇 |
2019年 | 36篇 |
2018年 | 25篇 |
2017年 | 42篇 |
2016年 | 34篇 |
2015年 | 62篇 |
2014年 | 55篇 |
2013年 | 67篇 |
2012年 | 77篇 |
2011年 | 73篇 |
2010年 | 45篇 |
2009年 | 50篇 |
2008年 | 52篇 |
2007年 | 51篇 |
2006年 | 47篇 |
2005年 | 31篇 |
2004年 | 29篇 |
2003年 | 23篇 |
2002年 | 25篇 |
2001年 | 26篇 |
2000年 | 16篇 |
1999年 | 9篇 |
1998年 | 5篇 |
1997年 | 6篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1992年 | 7篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1989年 | 3篇 |
1988年 | 4篇 |
1987年 | 6篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1971年 | 3篇 |
1966年 | 2篇 |
排序方式: 共有1018条查询结果,搜索用时 15 毫秒
81.
Castilho C Garcia JM Renesto A Nogueira GP Brito LF 《Animal reproduction science》2007,98(3-4):189-196
The objective of the present study was to characterize ovarian follicular dynamics and hormone concentrations during follicular deviation in the first wave after ovulation in Nelore (Bos indicus) heifers. Ultrasonographic exams were performed and blood samples were collected every 12h from the day of estrus until 120-144 h after ovulation in seven females. Deviation was defined as the point at which the growth rate of the dominant follicle became greater than the growth rate of the largest subordinate follicle. Deviation occurred approximately 65 h after ovulation. Growth rate of the dominant follicle increased (P<0.05) after deviation, while growth rate of the subordinate follicle decreased (P<0.05). Diameter of the dominant follicle did not differ from the subordinate follicle at deviation (approximately 5.4mm). The dominant follicle (7.6mm) was larger (P<0.05) than the subordinate follicle (5.3mm) 96 h after ovulation or 24h after deviation. Plasma FSH concentrations did not change significantly during the post-ovulatory period. The first significant increase in mean plasma progesterone concentration occurred on the day of follicular deviation. In conclusion, the interval from ovulation to follicular deviation (2.7 days) was similar to that previously reported in B. taurus females, but follicles were smaller. Diameters of the dominant follicle and subordinate follicle did not differ before deviation and deviation was characterized by an increase in dominant follicle and decrease in subordinate follicle growth rate. Variations in FSH concentrations within 12-h intervals were not involved in follicular deviation in Nelore heifers. 相似文献
82.
Nogueira FC Gonçalves EF Jereissati ES Santos M Costa JH Oliveira-Neto OB Soares AA Domont GB Campos FA 《Plant cell reports》2007,26(8):1333-1343
Using a combination of two-dimensional gel electrophoresis protein mapping and mass spectrometry analysis, we have established
proteome reference maps of embryogenic cell suspensions of cowpea (Vigna unguiculata). The cell suspensions were generated from young primary leaves and contained basically pro-embryogenic masses, which enabled
us to dissect their proteome composition while eliminating the complexity of too many cell types. Over 550 proteins could
reproducibly be resolved over a pI range of 3–10. A total of 128 of the most abundant protein spots were excised, digested
in-gel with trypsin and analyzed by tandem mass spectrometry. This enabled the identification of 67 protein spots. Two of
the most abundant proteins were identified as a chitinase and as a ribonuclease belonging to the family of PR-4 and PR-10
proteins, respectively. The expression of the respective genes was confirmed by RT-PCR and the pattern of deposition of the
PR-10 protein in cell suspensions as well as in developing cowpea seeds, roots, shoots and flowers were determined by Western
blot experiments, using synthetic antibodies raised against a 14-amino acid synthetic peptide located close to the C-terminal
region of the PR-10 protein. 相似文献
83.
Amarildo Emanuel Correia Jordão Bruno Cogliati Bernard Ernesto Jensch-Junior Leandro Nogueira Pressinotti Osório Miguel Parra Francisco Javier Hernandez-Blazquez José Roberto Machado Cunha da Silva 《Polar Biology》2007,30(3):387-390
The Notothenia coriiceps liver is commonly infected with parasites, reducing the hepatic mass and inducing the regeneration. In order to better understand
the effect of nutrient influx on hepatic regeneration at 0°C, a usual mammal hepatotrophic factor (HF) solution was injected
into ten fish (HF group), while ten fish were injected with saline solution (control), twice a day, for 15 days. The liver
and carcass weight were measured, and samples were obtained for histological studies. The HF group presented a higher liver/carcass
weight (62.5%) than control group. This increase in liver mass was due mainly to hepatocytes hypertrophy, including nuclear
size increase and cytoplasmic inclusions of glycogen. Hyperplasia is also observed, although to a lesser extent. The hepatic
reaction to HF in Antarctic fish was here demonstrated for the first time, helping to understand the liver response to seasonal
nutrient. 相似文献
84.
Barbara Ludwig Navarro Juan Pablo Edwards Molina Antonio Fernandes Nogueira Júnior 《Journal of Phytopathology》2022,170(1):57-68
Botryosphaeriaceae species have a wide host range and a worldwide distribution. These fungal species can colonize several plant organs, such as the trunk, leaves and fruit. Some Botryosphaeriaceae species cause important diseases on persimmon, avocado and guava fruit. However, there is a lack of information regarding the mechanisms of penetration by Botryosphaeriaceae species on these tropical and subtropical fruits. This study aimed to better understand the mechanisms involved in fungal penetration, host specificity and aggressiveness of Botryosphaeria dothidea, Lasiodiplodia pseudotheobromae and Neofusicoccum parvum on avocado (Persea americana), guava (Psidium guajava) and persimmon (Diospyros kaki) fruit. Scanning electron microscopy (SEM) image analysis showed that in avocado fruit, the three studied Botryosphaeriaceae species penetrated through lenticels. In guava fruit, penetration through stomata was verified for Botryosphaeria dothidea and Neofusicoccum parvum. In persimmon fruit, an appressoria-like structure was observed for B. dothidea, which suggests direct penetration. Disease incidence in wounded fruit was 24% higher than in non-wounded fruit. L. pseudotheobromae and N. parvum showed differences in aggressiveness in guava fruit. The longest incubation period was observed for N. parvum inoculated on guava, with an average of 4.5 days, and the shortest incubation period was verified for B. dothidea inoculated on avocado, with an average of 2.8 days. The area under the disease progress curve (AUDPC) did not differ between Botryosphaeriaceae species on avocado, whereas on guava and persimmon fruit, the AUDPC was lower for B. dothidea. The information regarding penetration mechanisms and aggressiveness is important to improve postharvest disease control strategies. 相似文献
85.
Length–weight relationships of fish from Madeira River,Brazilian Amazon,before the construction of hydropower plants
下载免费PDF全文
![点击此处可从《Zeitschrift fur angewandte Ichthyologie》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A. Cella‐Ribeiro M. Hauser L. D. Nogueira C. R. C. Doria G. Torrente‐Vilara 《Zeitschrift fur angewandte Ichthyologie》2015,31(5):939-945
Length–weight relationships (LWRs) are presented for 112 freshwater fish species representing 23 families and five orders captured in the Madeira River, the largest white‐water river tributary of the Amazon River. The allometry coefficient (b) of the LWR (Wt = aSLb) ranged from 2.446 to 3.856 with a median value of 3.102. Eight new LWR records are presented for Amazonian species as information for FishBase. LWRs in the present study provide historical data on a and b coefficients prior to the damming of the Madeira River in November 2011, allowing comparison estimates of predicted future population parameters as influenced by human intervention. 相似文献
86.
87.
Alex Pauvolid-Corrêa Raquel Soares Juliano Zilca Campos Jason Velez Rita Maria Ribeiro Nogueira Nicholas Komar 《Memórias do Instituto Oswaldo Cruz》2015,110(1):125-133
The Pantanal hosts diverse wildlife species and therefore is a hotspot for arbovirus
studies in South America. A serosurvey for Mayaro virus (MAYV), eastern (EEEV),
western (WEEV) and Venezuelan (VEEV) equine encephalitis viruses was conducted with
237 sheep, 87 free-ranging caimans and 748 equids, including 37 collected from a
ranch where a neurologic disorder outbreak had been recently reported. Sera were
tested for specific viral antibodies using plaque-reduction neutralisation test. From
a total of 748 equids, of which 264 were immunised with vaccine composed of EEEV and
WEEV and 484 had no history of immunisation, 10 (1.3%) were seropositive for MAYV and
two (0.3%) for VEEV using criteria of a ≥ 4-fold antibody titre difference. Among the
484 equids without history of immunisation, 48 (9.9%) were seropositive for EEEV and
four (0.8%) for WEEV using the same criteria. Among the sheep, five were sero-
positive for equine encephalitis alphaviruses, with one (0.4%) for EEEV, one (0.4%)
for WEEV and three (1.3%) for VEEV. Regarding free-ranging caimans, one (1.1%) and
three (3.4%), respectively, had low titres for neutralising antibodies to VEEV and
undetermined alphaviruses. The neurological disorder outbreak could not be linked to
the alphaviruses tested. Our findings represent strong evidence that MAYV and all
equine encephalitis alphaviruses circulated in the Pantanal. 相似文献
88.
89.
90.
Polyana C Tizioto Jeremy F Taylor Jared E Decker Caio F Gromboni Mauricio A Mudadu Robert D Schnabel Luiz L Coutinho Gerson B Mour?o Priscila SN Oliveira Marcela M Souza James M Reecy Renata T Nassu Flavia A Bressani Patricia Tholon Tad S Sonstegard Mauricio M Alencar Rymer R Tullio Ana RA Nogueira Luciana CA Regitano 《遗传、选种与进化》2015,47(1)