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41.
M.R. Reeve T.C. Cosper M.A. Walter 《Journal of experimental marine biology and ecology》1975,17(1):39-46
The passage of food through the gut of Sagitta hispida Conant is described as a batch process and the formation of membranes which surround the faecal material is demonstrated photographically. The utility of such a peritrophic membrane system is considered in the light of the marine ecosystem as a whole. 相似文献
42.
Members of social groups face a trade-off between investing selfish effort for themselves and investing cooperative effort to produce a shared group resource. Many group resources are shared equitably: they may be intrinsically non-excludable public goods, such as vigilance against predators, or so large that there is little cost to sharing, such as cooperatively hunted big game. However, group members'' personal resources, such as food hunted individually, may be monopolizable. In such cases, an individual may benefit by investing effort in taking others'' personal resources, and in defending one''s own resources against others. We use a game theoretic “tug-of-war” model to predict that when such competition over personal resources is possible, players will contribute more towards a group resource, and also obtain higher payoffs from doing so. We test and find support for these predictions in two laboratory economic games with humans, comparing people''s investment decisions in games with and without the options to compete over personal resources or invest in a group resource. Our results help explain why people cooperatively contribute to group resources, suggest how a tragedy of the commons may be avoided, and highlight unifying features in the evolution of cooperation and competition in human and non-human societies. 相似文献
43.
M. T. Johnson N. Greenwood D. B. Sivyer M. Thomson A. Reeve K. Weston T. D. Jickells 《Biogeochemistry》2013,113(1-3):23-36
The Cefas SmartBuoy network provides a unique insight into the biogeochemical dynamics of the Northern European shelf seas, particularly the North Sea, through high-resolution automated offshore water sampling. We present total dissolved nitrogen and dissolved organic nitrogen (DON) from the Dowsing SmartBuoy site (53.531° N, 1.053° E) from January to October 2010, the first high resolution seasonal (winter-autumn) cycle of DON from the open North Sea. On top of a refractory background DON concentration of approximately 5 μM, a rapid increase in DON of a further ~5 μM is observed over the course of the spring bloom. This rapidly produced DON declines at an estimated net decay rate of between 0.6 and 1.8 μM month?1. The slow decay suggests that the majority of the additional DON produced during the spring bloom is of semi-labile nature and has a lifetime of weeks to months. The dataset allows us to tightly constrain the budget for water column nitrogen over the winter, spring and summer of 2010 and clearly demonstrates the ‘sawtooth’ nature of the seasonal cycle of DON in the open North Sea, which has been impossible to resolve with a more traditional ship-based mode of operation. This work highlights the importance of autonomous sampling approaches in better understanding shelf sea biogeochemistry in the future. 相似文献
44.
Capsule Although the White Stork avoids adverse weather conditions by modifying its arrival and breeding, it cannot avoid extreme weather events during the breeding season.Aims To show how extreme weather conditions can influence breeding attempts of a large, long-lived species, the White Stork.Methods We analysed data on arrivals of White Storks in Western Poland from 2005 to 2013 and detailed breeding biology parameters from 2009 to 2013 in relation to weather conditions. We analysed breeding success and breeding failure rate from 1974 to 2013.Results In years with a cold March White Storks arrived later than when March was warmer. Frost during incubation negatively influenced the hatching success. Extreme weather events caused high late mortality even for nestlings older than 30 days. Data from 27 breeding seasons showed a significant increase in mean breeding success but also a significant increase in the proportion of pairs which lost broods in the nestling stage.Conclusion The White Stork can modify its arrival in response to current weather conditions on the breeding grounds but it cannot respond to extreme weather events. Due to increasing frequency of extreme weather events caused by climate change, White Stork breeding success may decrease in the future. 相似文献
45.
Ecosystems - Mangrove forests are vulnerable to accelerated sea-level rise associated with climate warming because they occupy a relatively narrow zone on the mid-to-upper-intertidal flats. The... 相似文献
46.
47.
Siyu Wang Stephanie M. Reeve Graham T. Holt Adegoke A. Ojewole Marcel S. Frenkel Pablo Gainza Santosh Keshipeddy Vance G. Fowler Dennis L. Wright Bruce R. Donald 《PLoS computational biology》2022,18(2)
Antimicrobial resistance presents a significant health care crisis. The mutation F98Y in Staphylococcus aureus dihydrofolate reductase (SaDHFR) confers resistance to the clinically important antifolate trimethoprim (TMP). Propargyl-linked antifolates (PLAs), next generation DHFR inhibitors, are much more resilient than TMP against this F98Y variant, yet this F98Y substitution still reduces efficacy of these agents. Surprisingly, differences in the enantiomeric configuration at the stereogenic center of PLAs influence the isomeric state of the NADPH cofactor. To understand the molecular basis of F98Y-mediated resistance and how PLAs’ inhibition drives NADPH isomeric states, we used protein design algorithms in the osprey protein design software suite to analyze a comprehensive suite of structural, biophysical, biochemical, and computational data. Here, we present a model showing how F98Y SaDHFR exploits a different anomeric configuration of NADPH to evade certain PLAs’ inhibition, while other PLAs remain unaffected by this resistance mechanism. 相似文献
48.
C.M. Turkelson T.E. Solomon L. Bussjaeger J. Turkelson M. Ronk J.E. Shively F.J. Ho J.R. Reeve Jr 《Peptides》1988,9(6):1255-1260
Cholecystokinin-58 (CCK-58) was purified from rat intestines using an extraction method that yields large amounts of this peptide. Greater than 30% of total CCK immunoreactivity eluted before CCK-39 upon gel permeation chromatography (Sephadex G-50) if extracts were loaded onto Sep Pak cartridges before freezing. If the extracts were frozen and stored at −70°C for six weeks, only 20% of the material eluted in this region and total immunoreactivity was reduced by 50%, suggesting that proteases were active under these storage conditions. This early eluting peak was purified by reverse phase and ion-exchange HPLC to a single absorbance peak. Microsequence analysis of this peak detected AVLRPDSEP which is the amino terminus of rat CCK-58 predicted from the rat preprocholecystokinin cDNA. Because degradation of CCK-58 occurred in these extracts, it is possible that CCK-58 is the predominant molecule form in the rat small intestine. 相似文献
49.
R Jiang V D Huebner T D Lee P Chew F J Ho J E Shively J H Walsh J R Reeve 《Peptides》1988,9(4):763-769
The heptadecapeptide form the rabbit gastrin was extracted from 16 rabbit antra and purified by a combination of DEAE Sephadex, C-18 SEP PAK cartridges, fast performance liquid chromatography (FPLC) and reverse phase high pressure liquid chromatography (HPLC) steps. After the HPLC purification, a sharp, single peak of gastrin-like immunoreactivity was detected that had the same absorption to immunoreactivity ratio as human gastrin. An amino terminal pyrrolidone carboxylic acid blocking group was removed by incubation with pyrrolidone carboxylic peptidase. The amino acid analysis, microsequence analysis and mass spectrometry all confirmed the structure of rabbit gastrin being pQGPWLQEEEEAYGWMDFamide. This sequence is identical to human gastrin-17 except for glutamine in position 6 which replaces glutamate in human gastrin. Both sulfated and unsulfated rabbit gastrin-17 were characterized by mass spectrometry. 相似文献
50.
Radioimmunoassays using antibodies specific for the carboxyl terminus of cholecystokinin (CCK) and the midportion of CCK-58 (raised against synthetic canine CCK-33-(1-27] revealed the existence of a CCK fragment in canine gut and brain extracts which lacks the biologically active carboxyl terminal immunoreactivity. This material eluted on Sephadex G-50 gel permeation chromatography in the region of CCK-58, on high-pressure liquid chromatography (HPLC) after CCK-39 and before CCK-58, and on cation-exchange FPLC it eluted after CCK-58. The immunoreactive pattern, the ratio of absorbance at 280-220 nm and the chromatographic elution positions suggest that this large CCK-like molecule represents an amino-terminal fragment of CCK-58. This fragment is present in canine gut and brain. Therefore, a similar processing site of procholecystokinin is suggested in both tissues. 相似文献