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71.
An opioid peptide, which shares similarity with mammalian hemorphins, has been identified from the synganglia (central nervous system) of the hard tick, Amblyomma testindiarium. Its primary sequence was established as LVVYPWTKM that contains a tetrapeptide sequence Tyr-Pro-Trp-Thr of hemorphin-like opioid peptides. By hot-plate bioassay, the purified peptide and synthetic peptide displayed dose-related antinociceptive effect in mice, as observed for other hemorphin-like opioid peptides. This is the first opioid peptide identified from ticks. Ticks may utilize the opioid peptide in their strategy to escape host immuno-surveillance as well as in inhibiting responses directed against themselves. 相似文献
72.
Bailey DM Genard B Collins MA Rees JF Unsworth SK Battle EJ Bagley PM Jamieson AJ Priede IG 《Physiological and biochemical zoology : PBZ》2005,78(3):335-346
Several complementary studies were undertaken on a single species of deep-sea fish (the eel Synaphobranchus kaupii) within a small temporal and spatial range. In situ experiments on swimming and foraging behaviour, muscle performance, and metabolic rate were performed in the Porcupine Seabight, northeast Atlantic, alongside measurements of temperature and current regime. Deep-water trawling was used to collect eels for studies of animal distribution and for anatomical and biochemical analyses, including white muscle citrate synthase (CS), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), and pyruvate kinase (PK) activities. Synaphobranchus kaupii demonstrated whole-animal swimming speeds similar to those of other active deep-sea fish such as Antimora rostrata. Metabolic rates were an order of magnitude higher (31.6 mL kg(-1) h(-1)) than those recorded in other deep-sea scavenging fish. Activities of CS, LDH, MDH, and PK were higher than expected, and all scaled negatively with body mass, indicating a general decrease in muscle energy supply with fish growth. Despite this apparent constraint, observed in situ burst or routine swimming performances scaled in a similar fashion to other studied species. The higher-than-expected metabolic rates and activity levels, and the unusual scaling relationships of both aerobic and anaerobic metabolism enzymes in white muscle, probably reflect the changes in habitat and feeding ecology experienced during ontogeny in this bathyal species. 相似文献
73.
74.
Structures of the iron-sulfur flavoproteins from Methanosarcina thermophila and Archaeoglobus fulgidus 下载免费PDF全文
Andrade SL Cruz F Drennan CL Ramakrishnan V Rees DC Ferry JG Einsle O 《Journal of bacteriology》2005,187(11):3848-3854
Iron-sulfur flavoproteins (ISF) constitute a widespread family of redox-active proteins in anaerobic prokaryotes. Based on sequence homologies, their overall structure is expected to be similar to that of flavodoxins, but in addition to a flavin mononucleotide cofactor they also contain a cubane-type [4Fe:4S] cluster. In order to gain further insight into the function and properties of ISF, the three-dimensional structures of two ISF homologs, one from the thermophilic methanogen Methanosarcina thermophila and one from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus, were determined. The structures indicate that ISF assembles to form a tetramer and that electron transfer between the two types of redox cofactors requires oligomerization to juxtapose the flavin mononucleotide and [4Fe:4S] cluster bound to different subunits. This is only possible between different monomers upon oligomerization. Fundamental differences in the surface properties of the two ISF homologs underscore the diversity encountered within this protein family. 相似文献
75.
Dylan Z. Childs C. J. E. Metcalf Mark Rees 《Proceedings. Biological sciences / The Royal Society》2010,277(1697):3055-3064
Understanding the adaptations that allow species to live in temporally variable environments is essential for predicting how they may respond to future environmental change. Variation at the intergenerational scale can allow the evolution of bet-hedging strategies: a novel genotype may be favoured over an alternative with higher arithmetic mean fitness if the new genotype experiences a sufficiently large reduction in temporal fitness variation; the successful genotype is said to have traded off its mean and variance in fitness in order to ‘hedge its evolutionary bets’. We review the evidence for bet-hedging in a range of simple plant systems that have proved particularly tractable for studying bet-hedging under natural conditions. We begin by outlining the essential theory, reiterating the important distinction between conservative and diversified bet-hedging strategies. We then examine the theory and empirical evidence for the canonical example of bet-hedging: diversification via dormant seeds in annual plants. We discuss the complications that arise when moving beyond this simple case to consider more complex life-history traits, such as flowering size in semelparous perennial plants. Finally, we outline a framework for accommodating these complications, emphasizing the central role that model-based approaches can play. 相似文献
76.
Kevin J. Flynn John A. Raven T. Alwyn V. Rees Zoe Finkel Antonietta Quigg John Beardall 《Journal of phycology》2010,46(1):1-12
The growth rate hypothesis (GRH) asserts, from known biochemistry, that maintaining high growth rates requires high concentrations of ribosomes. Since ribosomes are rich in phosphorus (P), the GRH predicts a positive correlation between growth rate and P content; this correlation is observed in some organisms. We consider the application of the GRH to phytoplankton and identify several key problems that require further research before the hypothesis can be accepted for these organisms. There are severe methodological problems that confound interpretation of data for testing the GRH. These problems include the measurement of protein and nucleic acids (such that ratio of these components carries a high level of uncertainty), studies of steady‐state versus dynamic systems, and the presentation of data per cell (especially as cell size varies with growth rate limitations) and the calculation of growth rates. In addition, because of the short generation times and rapid responses of these organisms to perturbations, ribosome and RNA content is expected to vary in response to (de)repression of various systems; content may increase on application of growth‐limiting stress. Finally, that most phytoplankton accumulate P when not P stressed conflicts with the GRH. In consequence, the value of the GRH for any sort of predictive role in nature appears to be severely limited. We conclude that the GRH cannot be assumed to apply to phytoplankton taxa without first performing experimental tests under transient conditions. 相似文献
77.
Reul Andreas; Rodriguez J.; Blanco J. M.; Rees A.; Burkill P. H. 《Journal of plankton research》2006,28(5):449-457
Water column structure, microphytoplankton size spectra andnutrient concentration were analysed at six sampling stationsin the Celtic Sea. Three types of stations were distinguished:(i) where the upper mixed layer (UML) reaches the total depth(TD), (ii) where the UML is about half of TD and (iii) wherethe UML is considerably less than half the TD. The UML was nutrientrich at type A stations and was nutrient depleted at type Bstations. At type C stations, the UML was nitrate depleted andsilicate rich. Two groups of microplankton size-abundance spectra(SAS) were found: (i) a typical linear SAS and (ii) a more complexatypical SAS, with a linear part up to 160 µmand a dome at 300 µm caused by a Coscinodiscus wailesiibloom. The dome was observed at all depths at type A stationsand above the pycnocline and at the seafloor of type B stations.Combining intrinsic growth rate, sinking rate and mixing layerdepth, the C. wailesii dome persists only at type A stationsbut settles out of the UML at the remaining stations. This suggeststhat a large part of the perturbation at the right extreme ofphytoplankton SAS does not propagate along the planktonic SASbut sinks to the seafloor.
This paper was presented in a session on "Size Structure ofPlankton Communities", at the ASLO Summer International Meeting,held in Santiago de Compostela, Spain, between 19 and 24 June,and coordinated by Xabier Irigoien, Roger Harris and Angel Lopez-Urrutia. 相似文献
78.
Kinetic analysis of the thermal stability of the photosynthetic reaction center from Rhodobacter sphaeroides 下载免费PDF全文
The temperature-induced denaturation of the photosynthetic reaction center from Rhodobacter sphaeroides has been studied through the changes that occur in the absorption spectrum of the bound chromophores on heating. At elevated temperatures, the characteristic absorbance bands of the bacteriochlorins bound to the polypeptides within the reaction center are lost, and are replaced by features typical of unbound bacteriochlorophyll and bacteriopheophytin. The kinetics of the spectral changes cannot be explained by a direct conversion from the functional to the denatured form of the protein, and require the presence of at least one intermediate. Possible mechanisms for the transformation via an intermediate are examined using a global analysis of the kinetic data, and the most likely mechanism is shown to involve a reversible transformation between the native state and an off-pathway intermediate, coupled to an irreversible transformation to the denatured state. The activation energies for the transformations between the three components are calculated from the effect of temperature on the individual rate constants, and the likely structural changes of the protein during the temperature-induced transformation are discussed. 相似文献
79.
Two popular methods of assessing lower body musculotendinous stiffness include the hopping and oscillation tests. The disparity and paucity of reliability data prompted this investigation into leg musculotendinous stiffness (Kleg) and ankle musculotendinous stiffness (Kank) measures. Kleg and Kank were assessed on three separate occasions in 20 female subjects. Kleg was determined using bilateral hopping procedures conducted at 2.2 Hz and 3.2 Hz frequencies. Kank was assessed by perturbation of the subject's ankle musculotendinous unit on an instrumented calf raise apparatus at 70% of maximum isometric force (MIF). Excellent reliability was produced for all Kleg measures between all days, whereas Kank exhibited acceptable reliability after one session of familiarization. No relationship was evident between Kleg and Kank. It was concluded that no familiarization session was required for Kleg at the test frequencies and conditions tested, whereas at least one familiarization session was needed to ensure the reliable assessment of Kank. 相似文献
80.
Devillers I Dive G De Tollenaere C Falmagne B de Wergifosse B Rees JF Marchand-Brynaert J 《Bioorganic & medicinal chemistry letters》2001,11(17):2305-2309
A series of imidazolopyrazinones 3, substituted at C-2, and C-2/C-6, has been prepared. The compounds behaved as quenchers of superoxide anion. The more active compounds are structurally related to coelenterazine, a natural substrate of marine bioluminescence. Theoretical parameters based on Hartree-Fock instabilities have been examined. 相似文献