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991.
992.
Suresh Reddy Mittapelli Shailendar Kumar Maryada Venkateswara Rao Khareedu Dashavantha Reddy Vudem 《Tree Genetics & Genomes》2014,10(2):399-409
The genus Citrus is an important fruit crop and nutritional source for the good health of humans. Cytochrome P450s represent about 1 % of the proteome and mediate diverse biochemical reactions pertaining to both primary and secondary metabolism. Analysis of Citrus genomic resources identified 296 plant cytochrome P450s (CYP) coding genes in Citrus clementina, 272 in double haploid (dh) Citrus sinensis, and 202 in C. sinensis. In C. clementina and dh C. sinensis, CYP genes are distributed into nine clans. In the three genomes, single intron containing CYP genes are predominant in the A-type families. Among non-A-type CYP families, multiple intron containing genes are predominant. More number of genes in CYP A-type families over non-A-type families is attributed to rapid evolution of A-type genes facilitated by their gene organization. Further, complex gene organization of non-A-type genes with the presence of multiple introns might have contributed to the slower evolvement of paralogs. Majority of introns (1,660) from three genomes showed canonical GT-AG splice sites. However, 33 introns showed non-conventional GC… PyAG splice sites and functionality of these splice sites is confirmed by the ESTs lacking this intron. Across the families, gene organization is conserved between the three genomes. In dh C. sinensis, 22 genes were identified to have alternate splicing. Examination of scaffolds in C. clementina revealed that majority of the Citrus CYP genes are solitary and a few of them are in clusters of 3–8 genes. PCR amplification of C. sinensis genomic DNA with gene-specific primers failed to amplify out-grouped genes Ccl-CYP706A16 and Ccl-CYP706B1, confirming that they are specific to C. clementina. Differential number of CYP genes observed between C. clementina and C. sinensis is attributed to the extent of variability between their parents representing ancestral taxa. 相似文献
993.
T. Satyanarayana P. Sreenivasulu A. S. Ratna D. V. R. Reddy M. V. Nayudu 《Journal of Phytopathology》1994,140(4):326-334
A virus disease characterized by chlorotic vein banding, chlorotic line pattern along the margins or midrib of mature leaflets and chlorotic spots/rings was observed on commercial groundnut crops in Rayalaseema area of Andhra Pradesh with an incidence from 1% to nearly 60%. The virus was transmitted by mechanical inoculation in extracts prepared with 0.01 M potassium phosphate butter, pH 8.0 to 21 species from the Chenopodiaceae, Cruciferae, Leguminosae and Solanaceae, Chenopodium quinoa was found to be a good local lesion host. The virus was neither seed-transmitted through 1591 groundnut seeds nor aphid-transmitted by Aphis craccivora, Myzus persicae and Rhopalosiphum maidis either in non-persistent or semi-persistent manner. The virus remained infective in buffered tobacco leaf sap at a dilution of 10?5; in a 10?1 dilution of buffered sap the virus was infective for 2–3 days at 22–29°C or when heated to 65°C for 10 min but not to 70°C. Clarification treatments with organic solvents with 10% chloroform was least damaging. The virus was purified from Nicotiana rustica leaves. Purified virus contained isometric particles of 51 nm in diameter with an electron dense core of 22 nm and two major polypeptides of 76 kDa and 36 kDa. A polyclonal antiserum to this virus was produced. In agar gel double diffusion, enzyme-linked immunosorbent assay and in electro-blot immunoassay rests the virus was related to peanut chlorotic streak virus and not to cauliflower mosaic, figwort mosaic and soybean chlorotic mottle viruses. 相似文献
994.
Effect of bacteria isolated from composts and macrofauna on sorghum growth and mycorrhizal colonization 总被引:1,自引:0,他引:1
B. Hameeda M. Srijana O. P. Rupela Gopal Reddy 《World journal of microbiology & biotechnology》2007,23(6):883-887
Summary Beneficial plant–microbe interactions in the rhizosphere are primary determinants of plant health and soil fertility. The
effect of combined inoculation of plant growth-promoting bacteria, Bacillus circulans EB 35, Serratia marcescens EB 67 and Pseudomonas sp. CDB 35 and arbascular mycorrhizal fungi, Glomus spp. on sorghum growth and mycorrhizal colonization was investigated. Plant growth observations taken at 45 days after sowing
(DAS) revealed that all the three strains applied along with arbascular mycorrhizae (AM) improved plant biomass from 17 to
20% and mycorrhizal colonization from 25 to 35%. Further studies at 90 DAS also showed improvement in plant growth parameters
measured. It was apparent that all the three strains stimulated plant and root growth in combination with AM and infection
of sorghum roots with mycorrhizae at 45 DAS was equal to or even greater than the AM + rock phosphate (RP) inoculation at
90 DAS. This shows the possible reduction of AM culturing period to 45 days compared to its 3-month culturing in the pot cultures. 相似文献
995.
Divya Kummari Kavi Kishor P. B. Bhatnagar-Mathur Pooja Singam Prashanth Sharma Kiran K. Vadez Vincent Reddy Palakolanu Sudhakar 《Molecular biology reports》2019,46(6):6039-6052
Molecular Biology Reports - Pearl millet is a C4 cereal crop that grows in arid and semi-arid climatic conditions with the remarkable abiotic stress tolerance. It contributed to the understanding... 相似文献
996.
997.
Holloway AC Melroe GT Ehrman MM Reddy PK Leatherland JF Sheridan MA 《American journal of physiology. Regulatory, integrative and comparative physiology》2000,279(2):R389-R393
In the present study, the effects of 17beta-estradiol (E(2)) treatment on the expression of preprosomatostatin (PPSS) I, PPSS II', and PPSS II" mRNA in the hypothalamus and endocrine pancreas (Brockmann body), as well as the effects of E(2) treatment on plasma somatostatin (SS)-14 and -25 concentrations in sexually immature rainbow trout (Oncorhynchus mykiss), were investigated. E(2) treatment significantly (P < 0.001) depressed both plasma SS-14 and SS-25. In the hypothalamus, E(2) treatment significantly (P < 0.001) decreased the levels of PPSS I and PPSS II" mRNA. However, there was no effect of E(2) treatment on PPSS II' mRNA levels. In the pancreas, E(2) treatment had no significant effect on the levels of either PPSS II' mRNA or PPSS II" mRNA. However, E(2) treatment significantly (P < 0.005) decreased levels of PPSS I mRNA. These data suggest that E(2) acts, in part, to increase plasma growth hormone levels in rainbow trout by decreasing the endogenous inhibitory somatostatinergic tone by inhibiting plasma levels of both SS-14 and SS-25 and hypothalamic levels of mRNA encoding these proteins. 相似文献
998.
999.
Intratesticular injection of prostaglandin E2 at a dose of 10 or 25 micrograms per testis caused desensitization of the testis to ornithine decarboxylase activity at 24 h after the injection. PGE2 caused desensitization in both Leydig cells and seminiferous tubules. The desensitized testis was refractory to follicle stimulating hormone, luteinizing hormone and cAMP in addition to PGE2. These results indicate that testicular desensitization to PGE2 is at a step beyond cAMP formation. 相似文献
1000.