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排序方式: 共有198条查询结果,搜索用时 109 毫秒
121.
Gen Suzuki Brian R. Weil Merced M. Leiker Amanda E. Ribbeck Rebeccah F. Young Thomas R. Cimato John M. Canty Jr 《PloS one》2014,9(11)
Background
Cardiosphere-derived cells (CDCs) improve ventricular function and reduce fibrotic volume when administered via an infarct-related artery using the “stop-flow” technique. Unfortunately, myocyte loss and dysfunction occur globally in many patients with ischemic and non-ischemic cardiomyopathy, necessitating an approach to distribute CDCs throughout the entire heart. We therefore determined whether global intracoronary infusion of CDCs under continuous flow improves contractile function and stimulates new myocyte formation.Methods and Results
Swine with hibernating myocardium from a chronic LAD occlusion were studied 3-months after instrumentation (n = 25). CDCs isolated from myocardial biopsies were infused into each major coronary artery (∼33×106 icCDCs). Global icCDC infusion was safe and while ∼3% of injected CDCs were retained, they did not affect ventricular function or myocyte proliferation in normal animals. In contrast, four-weeks after icCDCs were administered to animals with hibernating myocardium, %LADWT increased from 23±6 to 51±5% (p<0.01). In diseased hearts, myocyte proliferation (phospho-histone-H3) increased in hibernating and remote regions with a concomitant increase in myocyte nuclear density. These effects were accompanied by reductions in myocyte diameter consistent with new myocyte formation. Only rare myocytes arose from sex-mismatched donor CDCs.Conclusions
Global icCDC infusion under continuous flow is feasible and improves contractile function, regresses myocyte cellular hypertrophy and increases myocyte proliferation in diseased but not normal hearts. New myocytes arising via differentiation of injected cells are rare, implicating stimulation of endogenous myocyte regeneration as the primary mechanism of repair. 相似文献122.
Rana Begum Karin Calaza Jaimie Hoh Kam Thomas E. Salt Chris Hogg Glen Jeffery 《Biology letters》2015,11(3)
Ageing is an irreversible cellular decline partly driven by failing mitochondrial integrity. Mitochondria accumulate DNA mutations and reduce ATP production necessary for cellular metabolism. This is associated with inflammation. Near-infrared exposure increases retinal ATP in old mice via cytochrome c oxidase absorption and reduces inflammation. Here, we expose fruitflies daily to 670 nm radiation, revealing elevated ATP and reduced inflammation with age. Critically, there was a significant increase in average lifespan: 100–175% more flies survived into old age following 670 nm exposure and these had significantly improved mobility. This may be a simple route to extending lifespan and improving function in old age. 相似文献
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Ng KP Ngeow YF Yew SM Hassan H Soo-Hoo TS Na SL Chan CL Hoh CC Lee KW Yee WY 《Eukaryotic cell》2012,11(5):703-704
Daldinia eschscholzii is an invasive endophyte that is most commonly found in plant tissues rich in secondary metabolites. We report the draft genome sequence of D. eschscholzii isolated from blood culture. The draft genome is 35,494,957 bp in length, with 42,898,665 reads, 61,449 contigs, and a G+C content of 46.8%. The genome was found to contain a high abundance of genes associated with plant cell wall degradation enzymes, mycotoxin production, and antifungal drug resistance. 相似文献
125.
Ng KP Yew SM Chan CL Soo-Hoo TS Na SL Hassan H Ngeow YF Hoh CC Lee KW Yee WY 《Eukaryotic cell》2012,11(5):705-706
Cladosporium sphaerospermum is one of the most widely distributed allergens causing serious problems in patients with respiratory tract disease. We report the 26,644,473-bp draft genome sequence and gene annotation of C. sphaerospermum UM843. Analysis of the genome sequence led to the finding of genes associated with C. sphaerospermum's melanin biosynthesis, allergens, and antifungal drug resistance. 相似文献
126.
Ng KP Yew SM Chan CL Soo-Hoo TS Na SL Hassan H Ngeow YF Hoh CC Lee KW Yee WY 《Eukaryotic cell》2012,11(6):828
Pleosporales is the largest order in the fungal class Dothideomycetes. We report the 36,814,818-bp draft genome sequence and gene annotation of UM1110, a Pleosporales isolate associated with unclassified genera that is potentially a new fungal species. Analysis of the genome sequence led to the finding of genes associated with fungal adhesive proteins, secreted proteases, allergens, and pseudohyphal development. 相似文献
127.
Hannah S. Rhee Catherine M. Steel Frederik J. Derksen N. Edward Robinson Joseph F.Y. Hoh 《The journal of histochemistry and cytochemistry》2009,57(8):787-800
We used immunohistochemistry to examine myosin heavy-chain (MyHC)-based fiber-type profiles of the right and left cricoarytenoideus dorsalis (CAD) and arytenoideus transversus (TrA) muscles of six horses without laryngoscopic evidence of recurrent laryngeal neuropathy (RLN). Results showed that CAD and TrA muscles have the same slow, 2a, and 2x fibers as equine limb muscles, but not the faster contracting fibers expressing extraocular and 2B MyHCs found in laryngeal muscles of small mammals. Muscles from three horses showed fiber-type grouping bilaterally in the TrA muscles, but only in the left CAD. Fiber-type grouping suggests that denervation and reinnervation of fibers had occurred, and that these horses had subclinical RLN. There was a virtual elimination of 2x fibers in these muscles, accompanied by a significant increase in the percentage of 2a and slow fibers, and hypertrophy of these fiber types. The results suggest that multiple pathophysiological mechanisms are at work in early RLN, including selective denervation and reinnervation of 2x muscle fibers, corruption of neural impulse traffic that regulates 2x and slow muscle fiber types, and compensatory hypertrophy of remaining fibers. We conclude that horses afflicted with mild RLN are able to remain subclinical by compensatory hypertrophy of surviving muscle fibers. (J Histochem Cytochem 57:787–800, 2009) 相似文献
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Rebeccah J Mathews Michael B Sprakes Michael F McDermott 《Arthritis research & therapy》2008,10(6):228
The nucleotide-binding and oligomerization domain, leucine-rich repeat (also known as NOD-like receptors, both abbreviated
to NLR) family of intracellular pathogen recognition receptors are increasingly being recognized to play a pivotal role in
the pathogenesis of a number of rare monogenic diseases, as well as some more common polygenic conditions. Bacterial wall
constituents and other cellular stressor molecules are recognized by a range of NLRs, which leads to activation of the innate
immune response and upregulation of key proinflammatory pathways, such as IL-1β production and translocation of nuclear factor-κB
to the nucleus. These signalling pathways are increasingly being targeted as potential sites for new therapies. This review
discusses the role played by NLRs in a variety of inflammatory diseases and describes the remarkable success to date of these
therapeutic agents in treating some of the disorders associated with aberrant NLR function. 相似文献