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排序方式: 共有2206条查询结果,搜索用时 31 毫秒
71.
Rebeca Martínez Teresa Núñez de Villavicencio-Díaz Aniel Sánchez Yassel Ramos Jesús Noda Ferro Lázaro Gil González Milagros Méndez Elsa Rodríguez Ernesto Marcos Belinda Sánchez Yordanka Masforrol Hilda Garay Fernando Albericio Lisset Hermida Luis Javier González Eva Vonasek Mario P Estrada Vladimir Besada 《Biochemistry and Biophysics Reports》2016
BackgroundGrowth hormone secretagogues (GHS), among other factors, regulate the release of GH. The biological activity of the secretagogue peptide A233 as a promoter of growth and innate immunity in teleost fish has previously been demonstrated, but its role in the immune system of mammals is not well understood.MethodsThe effect of the peptide was investigated in J774A.2 macrophage cells using a comparative proteomics approach after 6 and 12 h of peptide stimulation.ResultsThe functional analysis of differentially modulated proteins showed that A233 peptide treatment appears to promote activation and ROS-dependent cytotoxic functions in macrophages and enhanced expression of antiviral protein complexes such as MAVS. In accordance with this hypothesis, we found that A233 treatment enhanced superoxide anion production and the IFN-γ level in J774A.2 cells and mouse splenocytes, respectively, and reduced viral load in a dengue virus mouse model of infection.ConclusionsThe growth hormone secretagogue A233 peptide promotes activation of ROS-dependent cytotoxic functions and exerts immunomodulatory effects that enable an antiviral state in a dengue virus mouse model.General SignificanceThe increase of IFN-γ level and the differential modulation of antiviral proteins by the A233 peptide suggest that the molecule could activate an innate immune response with a possible further impact in the treatment of acute and chronic diseases. 相似文献
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Juan Antonio Surez‐Cuenca Rogelio Robledo‐Nolasco Marco Antonio Alcntara‐Melndez Luis Javier Díaz Hernndez Eduardo Vera‐Gmez Alejandro Hernndez‐Patricio Karla Susana Snchez‐Díaz Juan Ariel Buendía‐Gutirrez Alejandra Contreras‐Ramos Atzin Su Ruíz‐Hernndez Rebeca Prez‐Cabeza de Vaca Paul Mondragn‐Tern 《Journal of cellular and molecular medicine》2019,23(7):4844-4849
75.
Michel Vidal 《Traffic (Copenhagen, Denmark)》2019,20(11):815-828
In recent years, the term “extracellular vesicle” (EV) has been used to define different types of vesicles released by various cells. It includes plasma membrane‐derived vesicles (ectosomes/microvesicles) and endosome‐derived vesicles (exosomes). Although it remains difficult to evaluate the compartment of origin of the two kinds of vesicles once released, it is critical to discriminate these vesicles because their mode of biogenesis is probably directly related to their physiologic function and/or to the physio‐pathologic state of the producing cell. The purpose of this review is to specifically consider exosome secretion and its consequences in terms of a material loss for producing cells, rather than on the effects of exosomes once they are taken up by recipient cells. I especially describe one putative basic function of exosomes, that is, to convey material out of cells for off‐site degradation by recipient cells. As illustrated by some examples, these components could be evacuated from cells for various reasons, for example, to promote “differentiation” or enhance homeostatic responses. This basic function might explain why so many diseases have made use of the exosomal pathway during pathogenesis. 相似文献
76.
González-Bergonzoni Ivan D’Anatro Alejandro Vidal Nicolás Stebniki Samanta Tesitore Giancarlo Silva Ivana Teixeira de Mello Franco 《Ecosystems》2019,22(8):1736-1753
Ecosystems - The origin of resources supporting metazoan biomass in rivers has long been a subject of debate. The river wave concept (RWC) postulates that the energetic basis of food webs varies... 相似文献
77.
Steven A. Li Melissa M. Cadelis Kenneth Sue Marine Blanchet Nicolas Vidal Jean Michel Brunel Marie-Lise Bourguet-Kondracki Brent R. Copp 《Bioorganic & medicinal chemistry》2019,27(10):2090-2099
The combination of increased incidence of drug-resistant strains of bacteria and a lack of novel drugs in development creates an urgency for the search for new antimicrobials. Initial screening of compounds from an in-house library identified two 6-bromoindolglyoxylamide polyamine derivatives (3 and 4) that exhibited intrinsic antimicrobial activity towards Gram-positive bacteria, Staphylococcus aureus and S. intermedius with polyamine 3 also displaying in vitro antibiotic enhancing properties against the resistant Gram-negative bacterium Pseudomonas aeruginosa. A series of 6-bromo derivatives (5–15) were prepared and biologically evaluated, identifying analogues with enhanced antibacterial activity towards Escherichia coli and with moderate to excellent antifungal properties. Polyamine 3, which includes a spermine chain, was the most potent of the series – its mechanism of action was attributed to rapid membrane permeabilization and depolarization in both Gram-positive and Gram-negative bacteria. 相似文献
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The cytoplasm-to-vacuole targeting (Cvt) pathway of Saccharomyces cerevisiae delivers aminopeptidase I (Ape1) from the cytosol to the vacuole, bypassing the normal secretory route. The Cvt pathway, although well-studied, was known only in S. cerevisiae. We demonstrate its existence in the methylotrophic yeast, Pichia pastoris, where it also delivers P. pastoris Ape1 (PpApe1) to the vacuole. Most proteins known to be required for the Cvt pathway in S. cerevisiae were, to the extent we found orthologs, also required in P. pastoris. The P. pastoris Cvt pathway differs, however, from that in S. cerevisiae, in that new proteins, such as PpAtg28 and PpAtg26, are involved. The discovery of a Cvt pathway in P. pastoris makes it an excellent model system for the dissection of autophagy-related pathways in a single organism and for the discovery of new Cvt pathway components. 相似文献
80.
Arias-Real Rebeca Gutirrez-Cnovas Cayetano Muoz Isabel Pascoal Cludia Menndez Margarita 《Ecosystems》2022,25(4):780-794
Ecosystems - Investigating the influence of biodiversity on ecosystem functioning over environmental gradients is needed to anticipate ecosystem responses to global change. However, our... 相似文献