Desorption of the cellulase systems of Trichoderma reesei and a Botrytis sp. from Avicel

Maximum desorptions (96%) of two microbial cellulolytic enzyme systems (Trichoderma reesei and a Botrytis sp.) from the Avicel were achieved using glycerol / diethyl ether (1:1, v/v). Then, using n-butanol, the cellulolytic enzyme systems were easily recovered from the glycerol / diethyl ether mixture. © Rapid Science Ltd. 1998     

Anti-amyloidogenic effect of AA3E2 attenuates β-amyloid induced toxicity in SK-N-MC cells

β-Amyloid peptide (Aβ) is believed to play a recognized role in pathogenesis of Alzheimer's disease (AD). Self-association of Aβ peptide into amyloid fibrils causes neurotoxicity. Compounds capable of interfering with Aβ–Aβ interaction through binding to nucleation sites can inhibit Aβ amyloidogenesis and Aβ-induced cytotoxicity. AA3E2 is a triazine-derivative whose anti-amyloidogenic ability has previously been established. In the present study, we evaluated the protective effect of AA3E3 against Aβ_1–42-induced toxicity in SK-N-MC cell line. The cell exposure to the co-incubated Aβ_1–42 with AA3E2 decreased the cell viability loss dose-dependently, compared to cells exposed to Aβ_1–42 fibrils.Co-incubation with AA3E2 also attenuated the ROS production, activation of caspase-3 and the extent of apoptotic cell death induced by Aβ_1–42 fibril. Moreover, the 3D structure of the molecular associates between Aβ_1–42 and AA3E2 were theoretically determined by docking studies. Our docking data indicated that AA3E2 inhibits the formation of Aβ fibril likely via binding to the nucleation site within the hydrophobic region of Aβ (KLVFF). These observations provide the background for future design of more elegant β-breaking agents for dissolution of Aβ fibrillar aggregates.     

Maternal Urinary Iodine Concentration and Pregnancy Outcomes: Tehran Thyroid and Pregnancy Study

Biological Trace Element Research - Long-term use of hair dyes has potential effects on metal content in hair. However, little research dissects the specific distribution and composition variations...