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991.
992.
Subhasish Purkayastha Gabriel Menichi Raymond P. Panzica 《Nucleosides, nucleotides & nucleic acids》2013,32(5-6):895-898
Abstract The preparation of 6 substituted pyrimidine nucleosides has received limited attention and undoubtedly reflects the difficulty in synthesizing nucleosides of this type. Condensation of & substituted pyrimidines with suitable sugar derivatives leads to the formation of mixtures of N3 and N1 nucleosides where the N3 isomer usually predominates1. This is exemplified by the direct ribosylation of the silyl derivative of 6-methyl-thiouracil, which furnished only the N3 ribonucleoside2. Ueda and coworkers' adcfessed this problem with moderate success. When 5′- O-acetyl-2′,3′-O-isopropylidine5bromouridine c1) was reacted with cyan- ide ion, a Michael-type addition occurred at C6 with concomitant dehycfo- brominatim to give the corresponding Gcyanowidine in quantitative yield. Treatment of 1(Scheme 1) with benzyl mercaptan, however furnished a 1:1 mixture of the C6 and C5 isomers 2 and 3 grespectively4. Attempts to alter the course of this reaction so that 2 predominated met with little success. It is worth mentioning that in ouFhands when this reaction was scaled-up, 3 predominated (2:3=1:4). Also the use of other sulfur nucleophiles, such as SEt, afforded only the C5-substituted derivative3. Thus, a new synthetic approach was sought which would furnish only the desired C6-substituted isomer and in reasonable yield. 相似文献
993.
994.
Summary Ca and Sr markedly inhibit the non-metabolic uptake of Na by the nonvacuolated tissue of maize root tips. Loss of previously absorbed Na is also reduced greatly in the presence of these ions. The results obtained suggest that in the absence of metabolically mediated ion transport the plasmalemma, stabilized by Ca-ions, is normally almost impermeable to Na and perhaps other ions. Ca appears to be slightly more effective than Sr in this regard.This report is based on work performed under Contract No. AT-(11-1)-34 Project 5 with the U.S. Atomic Energy Commission. 相似文献
995.
Characterization of an Unusual Strain of Proteus rettgeri Associated with an Outbreak of Nosocomial Urinary-Tract Infection 总被引:10,自引:1,他引:9
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W. H. Traub M. E. Craddock E. A. Raymond M. Fox C. E. McCall 《Applied microbiology》1971,22(3):278-283
An outbreak of nosocomial urinary-tract infection was caused by a strain of Proteus rettgeri that fermented lactose overnight and was resistant to all antimicrobial drugs tested. The nonmotile isolates shared an O (somatic) antigen that differed from those of wild-type P. rettgeri. The organisms proved markedly serum-sensitive. In rats, the isolates elicited an acute interstitial nephritis with associated transient bacteriuria. Attempts to transfer the lac(+) trait and drug-resistance markers to recipient strains of Escherichia coli K-12 failed; exposure of the isolates to acridine orange yielded small numbers of non-lactose-fermenting variants which, however, were still as drug-resistant as before. Epidemiological studies failed to uncover the source of this unique strain and appeared to indicate exogenous spread of infection. 相似文献
996.
Chris V. Bowen Drew DeBay H. Stephen Ewart Pamela Gallant Sean Gormley T. Toney Ilenchuk Umar Iqbal Tyler Lutes Marzia Martina Geoffrey Mealing Nadine Merkley Sandra Sperker Maria J. Moreno Christopher Rice Raymond T. Syvitski John M. Stewart 《PloS one》2013,8(3)
Soricidin is a 54-amino acid peptide found in the paralytic venom of the northern short-tailed shrew (Blarina brevicauda) and has been found to inhibit the transient receptor potential of vallinoid type 6 (TRPV6) calcium channels. We report that two shorter peptides, SOR-C13 and SOR-C27, derived from the C-terminus of soricidin, are high-affinity antagonists of human TRPV6 channels that are up-regulated in a number of cancers. Herein, we report molecular imaging methods that demonstrate the in vivo diagnostic potential of SOR-C13 and SOR-C27 to target tumor sites in mice bearing ovarian or prostate tumors. Our results suggest that these novel peptides may provide an avenue to deliver diagnostic and therapeutic reagents directly to TRPV6-rich tumors and, as such, have potential applications for a range of carcinomas including ovarian, breast, thyroid, prostate and colon, as well as certain leukemia''s and lymphomas. 相似文献
997.
Many cold‐adapted unicellular plants express ice‐active proteins, but at present, only one type of such proteins has been described, and it shows no resemblance to higher plant antifreezes. Here, we describe four isoforms of a second and very active type of extracellular ice‐binding protein (IBP) from a unicellular chlamydomonad alga collected from an Antarctic intertidal location. The alga is a euryhaline psychrophile that, based on sequences of the alpha tubulin gene and an IBP gene, appears to be the same as a snow alga collected on Petrel Island, Antarctica. The IBPs, which do not resemble any known antifreezes, have strong recrystallization inhibition activity and have an ability to slow the drainage of brine from sea ice. These properties, by maintaining liquid environments, may increase survival of the cells in freezing environments. The IBPs have a repeating TXT motif, which has previously been implicated in ice binding in insect antifreezes and a ryegrass antifreeze. 相似文献
998.
Theodore V. Fischer William E. Burkel Raymond H. Kahn Karl R. Herwig 《In vitro cellular & developmental biology. Plant》1976,12(5):382-392
Summary Organ cultures of rodent and human prostate glands have shown marked differences in their morphological response to testosterone.
In this study, explants from 19 canine prostate glands were cultivated for a minimum of 9 days in Trowell’s T-8 medium. Groups
of explants were exposed to media containing from 0.05 to 100 μm testosterone. While the higher testosterone levels (50 and
100 μm) markedly decreased explant viability, explants cultivated at lower levels (0.05 to 5 μm) appeared similar to control
explants in testosterone-free Trowell’s T-8 medium. Atmospheric mixtures containing either 95% or 50% oxygen were equally
effective.
Shortly after the cultures were initiated, large amounts of secretory product were liberated into the lumen. After 9 or more
days in vitro, glandular epithelium appeared cuboidal and never revealed the acid phosphatase-rich secretory granules seen
in the preculture control. However, the epithelium exhibited an increase in alkaline phosphatase and lipid content following
cultivation.
This project was supported by contract N01-CP-33331, Carcinogenesis Program, Division of Cancer Cause and Prevention, National
Cancer Institute, Bethesda, Maryland. 相似文献
999.
1000.