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S Daniels-McQueen A Ray W E Walden B K Ray P H Brown R E Thach 《Nucleic acids research》1988,16(15):7741
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Peter O''Connell G. Mark Lathrop Mark Leppert Yusuke Nakamura Ulrich Müller Jean-Marc Lalouel Ray White 《Genomics》1988,3(4):367-372
We have constructed a primary genetic map of human chromosome 18 consisting of 11 DNA markers and one serological marker (JK). Two of these loci define highly polymorphic VNTR systems. The markers define a continuous genetic linkage map of 97 cM in males and 205 cM in females; female genetic distances in a panel of 59 three-generation families were consistently about twice those observed in males. The high odds in support of the linear order of the markers on this recombination map, and the extent of coverage of chromosome 18, indicate that this map will permit efficient linkage studies of human genetic diseases that may be segregating on chromosome 18 and will provide anchor points for development of high-resolution maps for this chromosome. 相似文献
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Sixty-nine strains of bacteria isolated from murcha and ragi (amylase starters) were studied. These came from 14 different amylase starters from Java, Bali, and Nepal and were isolated from dilution plates incubated under aerobic and anaerobic conditions. Most belonged to Pediococcus , probably P. pentosaceus , and to Streptococcus faecalis. None were able to attack starch although they are used in starch fermentations. When inoculated on rice as pure cultures most of them failed to show visible growth unless yeasts and moulds were added at the same time. The roles of these bacteria are unknown but they may produce secondary products from the glucose formed by the amylolytic yeasts and moulds always found in the starters. 相似文献
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Immunization of mice with a synthetic GM3-lactam-BSA (bovine serum albumin) conjugate (designed to emulate the corresponding natural GM3-lactone conjugate), followed by fusion of splenocytes with myeloma cells, gave rise to more than 300 monoclonal hybridomas producing antibodies to GM3-lactam-BSA, which did not react with Glc-BSA and BSA. Eight antibody clones were randomly chosen from the positive 300 hybridomas. The eight clones, all belonging to the IgG class, were unreactive against GM3-ganglioside, whereas two antibodies (P5-1 and P5-3, both IgG1, ) reacted with GM3-ganglioside lactone. Binding of these two antibodies to the GM3-lactam-BSA conjugate was inhibited by soluble glycosides of GM2-, GM3-, and GM4-lactam and by GM3- and GM4-lactam, respectively, but not by Gb3 or asialo-GM1 and GM2-saccharides. A third antibody (P3; IgG2b, ) was inhibited by GM2-, GM3-, and GM4-lactam, but did not recognize GM3-ganglioside lactone. 相似文献