全文获取类型
收费全文 | 265篇 |
免费 | 21篇 |
出版年
2022年 | 3篇 |
2021年 | 5篇 |
2020年 | 2篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2017年 | 5篇 |
2016年 | 5篇 |
2015年 | 20篇 |
2014年 | 11篇 |
2013年 | 11篇 |
2012年 | 27篇 |
2011年 | 18篇 |
2010年 | 13篇 |
2009年 | 10篇 |
2008年 | 15篇 |
2007年 | 14篇 |
2006年 | 11篇 |
2005年 | 13篇 |
2004年 | 22篇 |
2003年 | 9篇 |
2002年 | 16篇 |
2001年 | 1篇 |
2000年 | 3篇 |
1999年 | 5篇 |
1998年 | 2篇 |
1997年 | 5篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 2篇 |
1984年 | 5篇 |
1983年 | 1篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1965年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有286条查询结果,搜索用时 93 毫秒
281.
Using the technique of indirect immunofluorescence, thyrotropin-releasing hormone (TRH) has been localised in certain cells of the cutaneous glands of the frog, . No specific fluorescence was found in other cells in the dermis, nor were any positive cells found in the epidermis. Previous studies have shown by radioimmunoassay that large amounts of TRH are present in frog skin, particularly in the dorsal region. Since dorsal frog skin contains many more glands than its ventral counterpart, this non-homogeneous distribution of TRH can be explained by its presence in a population of cells found in one type of cutaneous gland. 相似文献
282.
Avi Eldar Claudio Ghittino Liliana Asanta Elena Bozzetta Mariella Goria Marino Prearo Herve Bercovier 《Current microbiology》1996,32(2):85-88
The reference strains of Enterococcus seriolicida (ATCC 49156T) (T = type strain) and of Lactococcus garvieae (ATCC 43921T) and 30 field strains of Gram-positive cocci isolated from diseased rainbow trout in Italy were found to be phenotypically
(API 20 STREPT and API 50 CH) and genetically (DNA-DNA hybridization) similar. The high DNA-DNA homologies (70–100%) and the
low ΔTm(e) (less than 1.1°C) among these strains showed that Enterococcus seriolicida and Lactococcus garvieae are synonyms, describing a single bacterial species. E. seriolicida strains should be classified as L. garvieae, which must be considered as a major pathogen of freshwater and salt water fish with a world-wide distribution. 相似文献
283.
Frédéric Delsuc Mariella Superina Guillermo Ferraris Marie-Ka Tilak Emmanuel J. P. Douzery 《Conservation Genetics》2007,8(2):503-507
In a private wildlife research facility and rhea farm of Argentina, artificially incubated eggs of putative hybrid origin
between the Greater Rhea (Rhea americana) and the Lesser Rhea (R. pennata) hatched and gave birth to healthy chicks. Molecular genotyping by the analysis of mitochondrial Cytochrome b (Cyt b) and nuclear Chromo-Helicase DNA binding (CHD1) gene sequences confirmed the hybrid origin of these chicks which were molecularly
sexed as females. The possibility of hybridisation argues for careful management of captive populations of these species,
especially if individuals are to be released in the wild for conservation purposes. 相似文献
284.
Mariella Nieddu Roberto Mezzanotte Giuseppina Pichiri Pier Paolo Coni Gian Luca Dedola Maria Luisa Dettori Michele Pazzola Giuseppe Massimo Vacca Renato Robledo 《Genetics and molecular biology》2015,38(4):513-518
Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis
musimon, were used as probes for hybridization on genomic DNA and on
metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are
sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families.
Southern blots and fluorescence in situ hybridization experiments
showed completely coherent results: the Bovini probe Bt1 hybridized only to members
of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1
hybridized only to members of the Caprini tribe and not to members of Bovini.
Hybridization signals were detected in the heterochromatic regions of every
acrocentric autosome, except for two pairs of autosomes from Capra
hircus that did not show hybridization to probe Om1. No signal was
detected on X and Y chromosomes or on bi-armed autosomes. Remarkably, probe Om1
showed almost 100% homology with a bacterial sequence reported in
Helicobacter pylori. 相似文献
285.
Andrea Trevisan Isabella Maccà Francesca Rui Mariella Carrieri Giovanni Battista Bartolucci Maurizio Manno 《Biomarkers》2013,18(6):399-409
Blood and urine perchloroethylene and urine trichloroacetic acid, as markers of exposure, and serum AST, ALT, GGT and creatinine, urine total solutes and proteins, angiotensin converting enzyme, N-acetyl-ß-D-glucosaminidase and glutamine synthetase, as markers of effect, were measured in 40 dry-cleaning and 45 ironing-shop female workers. Average perchloroethylene air level in the dry-cleaning shops was 59.7 mg m-3, i.e. three-fold lower than the current A.C.G.I.H. TLV-TWA (170 mg m-3). No statistically significant difference in the mean values of any of the effect markers was observed between the two groups, except for AST which was significantly higher in drycleaners. In addition, a statistically significant correlation was observed in dry-cleaners between environmental perchloroethylene and total urinary solutes (r = 0.308, p < 0.05) or urine glutamine synthetase (r= 0.469, p < 0 .01), between glutamine synthetase and blood perchloroethylene in post-shift (r= 0.406, p < 0.01) or urinary perchloroethylene in post(r= 0.571, p < 0.001) or pre-shift (r= 0.586, p < 0.001), and between urinary perchloroethylene in pre-shift and GGT (r= 0.407, p < 0.05). Interestingly, some statistically significant correlations between exposure and effect indices were found in ironing-shop workers alone, as in all subjects. Finally, transaminases, GGT and total urinary proteins were influenced by age and alcohol consumption which were significantly higher in dry-cleaners, thus providing an explanation for some of the correlations observed. In conclusion, our results show a dose-related increase of glutamine synthetase activity,a marker of damage of the pars recta of the kidney proximal tubule, in the urine of female subjects exposed to perchloroethylene concentrations in the work environment lower than current A.C.G.I.H. TLV-TWA. 相似文献
286.
M Ravazzola D Baetens R Engerman N Kovacevic M Vranic L Orci 《Hormones et métabolisme》1977,9(6):480-483
Immunofluorescence shows that the oxyntic mucosa of a dog depancreatized for 5 years and having a poorly-controlled diabetes has more glucagon- and somatostatin-containing cells than the mucosa of a control dog. At the ultrastructural level, 4 endocrine cell types are identified: A-, A-like, D- and enterochromaffin-like (ECL) cells, with increased numbers of A-, A-like and D-cells in gastric glands of the depancreatized dog, together with a higher concentration of immunoreactive glucagon in the gastric mucosa. The increase in A-, A-like and D-cells is compatible with: a) a change induced by the diabetic state itself; b) a hyperplasia secondary to the loss of corresponding pancreatic cells. At any rate, the fact that A-, A-like and D-cells increase parallely may indicate that these three cell types are functionally related one with another. 相似文献