排序方式: 共有33条查询结果,搜索用时 15 毫秒
21.
Vylassery Deepthi Bawankar Raksha Singh Pooja Rathinasamy Subashkumar Govindasamy Vivekanandhan Ramamoorthy Siva 《Archives Of Phytopathology And Plant Protection》2013,46(11):1390-1399
Seedlings of cultivated rice variety ADT43 was investigated after challenging with two different abiotic (drought and salinity) and biotic (sheath blight and bacterial leaf blight pathogens) stresses. Salinity and drought stress reduced the growth of seedlings, mainly the higher conditions (100 mM NaCl and 10?days of drought, respectively). Increased level of MDA content was observed in biotic and abiotic-stress treated seedlings. The highest H2O2 content was observed under salinity-stressed seedlings and lower level observed under biotic stress. Superoxide dismutase activity showed a gradual decrease in all stress conditions compared to control. Salinity stress resulted in highest activity of catalase compared to biotic stress. The peroxidase activity of the seedlings was found to be increased under salt and drought stress conditions and the activity decreased under biotic stress. Drought stress resulted in induced expression of POC1 gene whereas the biotic stress showed lower expression level. Suppression of the rice peroxidase would have been the mechanism of overcoming the intrinsic defence in rice by these pathogens. 相似文献
22.
Nathan Vinod Kumar Mary Esther Rani Rathinasamy Gunaseeli Narayanan Dhiraviam Kannan Jayavel Sridhar 《Bioinformation》2012,8(22):1105-1110
Cellulase is one of the most widely distributed enzymes with wide application. They are involved in conversion of biomass into
simpler sugars. Cellulase of Trichoderma longibrachiatum, a known cellulolytic fungus was compared with Clostridium thermocellum
[] cellulase. Blastp was performed with AAA23226.1 as query sequence to obtain nine similar sequences from NCBI
protein data bank. The physicochemical properties of cellulase were analyzed using ExPASy’s ProtParam tool namely ProtParam,
SOPMA and GOR IV. Homology modeling was done using SWISS MODEL and checked quality by RMSD values using VMD1.9.1.
Active sites of each model were predicted using automated active site prediction server of SCFBio. Study revealed instability of
cellulase of two eukaryotic strains namely Trichoderma longibrachiatum [ AAA23226.1] and Melanocarpus albomyces [ CAA43059.1]. The
negative GRAVY score value of cellulases ensured better interaction and activity in aqueous phase. It was found that molecular
weight (M. Wt) ranges between 25-127.56 kDa. Iso-electric point (pI) of cellulases was found to be acidic in nature. GOR IV and
SOPMA were used to predict secondary structure of cellulase, which showed that random coil, was dominated. Neighbor joining
tree with C. thermocellum [ CAD56665.1] cellulase as root showed that cellulases of Thermoaerobacter subterraneus [ AAA23226.1] and
C. thermocellum [CAA4305.1] were more similar to eukaryotic cellulases supported by least boot strap values. Pseudoalteromonas
haloplanktis cellulase was found to be the ideal model supported by least RMSD score among the predicted structures. Trichoderma
longibrachiatum cellulase was found to be the best compared to other cellulases, which possess high number of active sites with
ASN and THR rich active sites. CYS residues were also present ensuring stable interaction and better bonding. Hydrophilic
residues were found high in active sites of all analyzed models and template. ZP_07835928相似文献
23.
A web accessible resource for investigating cassava phenomics and genomics information: BIOGEN BASE 总被引:1,自引:0,他引:1
Jayakodi M Selvan SG Natesan S Muthurajan R Duraisamy R Ramineni JJ Rathinasamy SA Karuppusamy N Lakshmanan P Chokkappan M 《Bioinformation》2011,6(10):391-392
The goal of our research is to establish a unique portal to bring out the potential outcome of the research in the Casssava crop. The Biogen base for cassava clearly brings out the variations of different traits of the germplasms, maintained at the Tapioca and Castor Research Station, Tamil Nadu Agricultural University. Phenotypic and genotypic variations of the accessions are clearly depicted, for the users to browse and interpret the variations using the microsatellite markers. Database (BIOGEN BASE - CASSAVA) is designed using PHP and MySQL and is equipped with extensive search options. It is more user-friendly and made publicly available, to improve the research and development of cassava by making a wealth of genetics and genomics data available through open, common, and worldwide forum for all individuals interested in the field. AVAILABILITY: The database is available for free at http://www.tnaugenomics.com/biogenbase/casava.php. 相似文献
24.
Parthasarathy NJ Kumar RS Manikandan S Narayanan GS Kumar RV Devi RS 《Chemico-biological interactions》2006,161(1):14-25
It is well known that the nervous system has increased susceptibility to methanol intoxication. The present study reveals the effect of methanol intoxication on antioxidant status, lipid peroxidation and DNA integrity in hypothalamic-pituitary-adrenal (HPA) axis organs and spleen. Non-specific and specific immune functions were analyzed. In addition, open field behavior, plasma corticosterone level and blood methanol level were estimated. Male Wistar albino rats were intoxicated with methanol (2.37 g/kg b.wt., i.p.) for 1 day, 15 and 30 days. Administration of methanol showed significant increase in enzymatic (superoxide dismutase, catalase, glutathione peroxidase), non-enzymatic (reduced glutathione and Vitamin C) antioxidants and lipid peroxidation (LPO) in hypothalamus and adrenal gland of day 1 group. However, decrease in enzymatic and non-enzymatic antioxidants with concomitant increase in LPO level were observed in 15 and 30 days groups. Plasma corticosterone level was significantly increased in day 1 and 15 days groups whereas, 30 days methanol intoxication group showed considerable decrease in corticosterone level compared with control animals. Cell-mediated immune response of footpad thickness was significantly decreased with an increased leukocyte migration inhibition. Humoral immune response of antibody titers was elevated in methanol-intoxicated groups. Neutrophil functions, adherence and phagocytic index (PI) were found to be significantly decreases. Furthermore, significant increase in the avidity index and nitro blue tetrozolium reduction was observed in the methanol exposed animals. Day 1 methanol exposed group showed increased PI compared to the control ones. Methanol exposure for 30 days showed an increased DNA fragmentation in the hypothalamus, adrenal glands, and spleen. In conclusion, exposure to methanol-induced oxidative stress disturbs the HPA-axis function altering the level of corticosterone, which lead to varied non-specific and specific immune response in experimental rats. 相似文献
25.
Quantitative cellular uptake, localization and cytotoxicity of curcumin in normal and tumor cells 总被引:2,自引:0,他引:2
Kunwar A Barik A Mishra B Rathinasamy K Pandey R Priyadarsini KI 《Biochimica et biophysica acta》2008,1780(4):673-679
Using absorption and fluorescence spectroscopic methods, quantitative cellular uptake of curcumin, an antioxidant and anti-tumor agent from Curcuma longa, was calculated in two types of normal cells: spleen lymphocytes, and NIH3T3 and two tumor cell lines: EL4 and MCF7. Both the uptake and fluorescence intensity of curcumin were significantly higher in tumor cells compared to the normal cells. A linear dependency on the uptake was observed with treatment concentration of curcumin. Using laser confocal microscopy, intracellular localization of curcumin was monitored and the results indicated that curcumin is located both in the cell membrane and the nucleus. Sub-cellular fractionation of curcumin-loaded MCF7 cells supported the differential distribution of curcumin in membrane, cytoplasm and nuclear compartments of cell with maximum localization in the membrane. Cytotoxicity studies in different cell lines indicated that the toxicity of curcumin increased with increasing uptake. 相似文献
26.
Microtubules, composed of alphabeta tubulin dimers, are dynamic polymers of eukaryotic cells. They play important roles in various cellular functions including mitosis. Microtubules exhibit differential dynamic behaviors during different phases of the cell cycle. Inhibition of the microtubule assembly dynamics causes cell cycle arrest leading to apoptosis; thus, qualifying them as important drug targets for treating several diseases including cancer, neuronal, fungal, and parasitic diseases. Although several microtubule-targeted drugs are successfully being used in cancer chemotherapy, the development of resistance against these drugs and their inherent toxicities warrant the development of new agents with improved efficacy. Several antimicrotubule agents are currently being evaluated for their possible uses in cancer chemotherapy. Benomyl, griseofulvin, and sulfonamides have been used as antifungal and antibacterial drugs. Recent reports have shown that these drugs have potent antitumor potential. These agents are shown to inhibit proliferation of different types of tumor cells and induce apoptosis by targeting microtubule assembly dynamics. However, unlike vincas and taxanes, which inhibit cancer cell proliferation in nanomolar concentration range, these agents act in micromolar range and are considered to have limited toxicities. Here, we suggest that these drugs may have a significant use in cancer chemotherapy when used in combination with other anticancer drugs. 相似文献
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Oxaliplatin resistance in colorectal cancer cells is mediated via activation of ABCG2 to alleviate ER stress induced apoptosis
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Acute hypoxic preconditioning prevents palmitic acid‐induced cardiomyocyte apoptosis via switching metabolic GLUT4‐glucose pathway back to CD36‐fatty acid dependent
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