Regulation of growth in rice seedlings

Etiolated rice seedlings (Oryza sativa L.) exhibited marked morphological differences when grown in sealed containers or in containers through which air was passed continuously. Enhancement of coleoptile and mesocotyl growth and inhibition of leaf and root growth in the sealed containers (enclosure syndrome) were accompanied by accumulation of CO₂ and C₂H₄ in and depletion of O₂ from the atmosphere. Ethylene (1 l 1^–1), high levels of CO₂, and reduced levels of O₂ contributed equally to the increase in coleoptile and mesocotyl growth. The effect of enclosure could be mimicked by passing a gas mixture of 3% O₂, 82% N₂, 15% CO₂ (all v/v), and 1 l l^–1) C₂H₄ through the vials containing the etiolated seedlings. The effects of high CO₂ and low O₂ concentrations were not mediated through increased C₂H₄ production. The enclosure syndrome was also observed in rice seedlings grown under water either in darkness or in light. The length of the rice coleoptile was positively correlated with the depth of planting in water-saturated vermiculite. The length of coleoptiles of wheat, barley, and oats was not affected by the depth of planting. In rice, the length of coleoptile was determined by the levels of O₂, CO₂, and ethylene, rather than by light. This regulatory mechanism allows rice seedlings to grow out of shallow water in which the concentration of O₂ is limiting.     

Endothelial cell junctions

In the course of a freeze-cleave study on intercellular junctions in the regenerating rat liver, we observed an unusual array of intramembranous particles located in regions of contact between endothelial cells lining the hepatic sinusoids. These arrays were characterized by an accumulation of particles which resembled a zonula occludens in their linear deployment but differed in that the contact regions were composed of individual particles which remained separated from each other by regular particle-free intervals.     

Sexual reproduction in the arum lily family,with emphasis on thermogenicity

Summary Floral thermogenicity, which is found in several representatives of half a dozen angiosperm families, is most pronounced in the Araceae. It is based on the operation of an alternative, cyanide-resistant electron transport chain which, in contrast to the classic cytochrome oxidase system, produces little ATP; most of the energy originally locked up in the respiratory substrate usually starch — is therefore liberated in the form of heat. The biological function of this (biochemically wasteful) system is to release the heat to serve as a volatilizer for the floral odors (often containing aliphatic amines, indole and skatole) that attract the insect pollinators. This makes the survival value of thermogenesis (for the plant species) immediately clear. Thermogenicity is under tight biological control, as demonstrated by the fact that the same ceiling temperature is always reached, regardless of ambient temperature. In Eastern skunk cabbage (Symplocarpus foetidus), which flowers very early in spring, that ceiling is about 20° C, in tropical forms such as Xanthosoma robustum and Philodendron selloum, it lies in the 42°–44° C range. In several instances, e.g., in Arum and in Sauromatum, the voodoo lily, thermogenicity manifests itself as a flare-up of only a few hours' duration, a respiratory explosion that can lead to rates of metabolism that compare favorably with those of a hovering hummingbird. The metabolic peak is always reached at a particular time of day, which is different for the different arum lily species, and thus reduces competition for pollinators. The odors that accompany the heat are also very characteristic, appealing to different pollinator classes and further reducing such competition. In the voodoo lily and in Arum, the primary site for the production of both heat and odor is the naked appendix of the inflorescence, which acts as a specialized osmophore or odor carrier. The first explosion may be followed by another one several hours later, which manifests itself in the floral chamber of the inflorescence and is under strict photoperiodic control. In Sauromatum, the first metabolic explosion is triggered by a plant hormone, originally referred to as calorigen, which originates in the primordia of the staminate flowers and moves from there into the appendix where it exerts its action after a lag-time of about a day — an indication that synthesis of new enzymatic protein (through unblocking of certain genes?) may well be involved. In 1987, calorigen was shown to be identical with salicylic acid. This compound was already known to induce flowering in certain duck-weeds, Lemnaceae, which until recently were regarded as belonging to the same family as arum lilies. In certain water lilies (Nymphaeaceae), thermogenicity is combined with a pollination syndrome very similar to that of Arum and Sauromatum but involving temporary trap flowers.Dedicated to the memory of A.W.H. van Herk, pioneer in the study of arum lilies, superb lecturer, and unselfish human being     

Cryopreserved human fetal pancreas: a source of insulin-producing tissue?

Human fetal pancreata (HFP) were obtained from dilatation and extraction aborted fetuses of 11-18 weeks' gestation. The pancreas was excised under sterile conditions and kept in culture medium at 4 degrees C, prior to stepwise digestion into 50- to 150-micron fragments. The fragmented pieces were allowed to sediment by gravity, then transferred to tissue culture for 24-48 h, and cryopreserved. The freeze-thaw protocol used stepwise equilibration with dimethyl sulfoxide, nucleation of the sample at -10 degrees C, and a slow cooling rate of 0.25 degrees C/min to -40 degrees C, followed by submersion in liquid nitrogen (-196 degrees C). Rapid thawing at 300 degrees C/min from -196 degrees C was employed. Both fresh and frozen-thawed HFP fragments appeared viable as judged by light and electron microscopy, and secreted insulin in a perifusion system upon stimulation with glucose (28 mM) and theophylline (10 mM) or glucose (2.8 mM) and theophylline (10 mM). Six patients with Type I insulin-dependent diabetes mellitus, already requiring immunosuppression for a kidney transplant, had intraportal injection of 20 cryopreserved-thawed and pooled HFP fragments. Up to the 1-year post-transplant follow-up, there has been no evidence of in vivo insulin or C-peptide production. The usefulness of cryopreserved human fetal pancreata as a source of insulin-producing tissue for diabetic patients, therefore, remains to be demonstrated.     

Regulation of growth in stem sections of deep-water rice

Submergence in water greatly stimulates internodal elongation in excised stem sections of deep-water rice (Oryza sativa L. cv. Habiganj Aman II) and inhibits growth of leaf blades and leaf sheaths. The highest rates of internodal growth have been observed in continuous light. Very little growth occurs in submerged sections kept in darkness or incubated under N₂ in the light. The effect of submergence on the growth of deep-water rice is, at least in part, mediated by C₂H₄, which accumulates in the air spaces of submerged sections. This accumulation results from increased C₂H₄ synthesis in the internodes of submerged sections and reduced diffusion of C₂H₄ from the tissue into the water. Increased C₂H₄ levels accelerate internodal elongation and inhibit the growth of leaves. Compounds capable of changing the rate of C₂H₄ synthesis, namely aminoethoxyvinylglycine, an inhibitor of C₂H₄ synthesis, and 1-aminocyclopropane-1-carboxylic acid, the immediate, precursor of C₂H₄, have opposite effects on growth of internodes and leaves. The enhancement of internodal elongation by C₂H₄ is particularly pronounced in an atmosphere of high CO₂ and low O₂. The increase in C₂H₄ synthesis in internodes of submerged sections is primarily triggered by reduced atmospheric concentrations of O₂. The rate of C₂H₄ evolution by internodes isolated from stem sections and incubated in an atmosphere of low O₂ is up to four times greater than that of isolated internodes incubated in air. In contrast, C₂H₄ evolution from the leaves is reduced under hypoxic conditions. The effect of submergence on growth of stem sections of deep-water rice can be mimicked by exposing non-submerged sections to a gas mixture which is similar to the gaseous atmosphere in the internodal lacunae of submerged sections, namely 3% O₂, 6% CO₂, 91% N₂ (by vol.) and 1 l l^-1 C₂H₄. Our results indicate that growth responses obtained with isolated rice stem sections are similar to those of intact deep-water rice plants.Abbreviations ACC 1-aminocyclopropane-1-carboxylic acid - AVG aminoethoxyvinylglycine