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排序方式: 共有183条查询结果,搜索用时 837 毫秒
31.
Engineered Polyamine Catabolism Preinduces Tolerance of Tobacco to Bacteria and Oomycetes 总被引:1,自引:0,他引:1
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Panagiotis N. Moschou Panagiotis F. Sarris Nicholas Skandalis Athina H. Andriopoulou Konstantinos A. Paschalidis Nickolas J. Panopoulos Kalliopi A. Roubelakis-Angelakis 《Plant physiology》2009,149(4):1970-1981
Polyamine oxidase (PAO) catalyzes the oxidative catabolism of spermidine and spermine, generating hydrogen peroxide. In wild-type tobacco (Nicotiana tabacum ‘Xanthi’) plants, infection by the compatible pathogen Pseudomonas syringae pv tabaci resulted in increased PAO gene and corresponding PAO enzyme activities; polyamine homeostasis was maintained by induction of the arginine decarboxylase pathway and spermine was excreted into the apoplast, where it was oxidized by the enhanced apoplastic PAO, resulting in higher hydrogen peroxide accumulation. Moreover, plants overexpressing PAO showed preinduced disease tolerance against the biotrophic bacterium P. syringae pv tabaci and the hemibiotrophic oomycete Phytophthora parasitica var nicotianae but not against the Cucumber mosaic virus. Furthermore, in transgenic PAO-overexpressing plants, systemic acquired resistance marker genes as well as a pronounced increase in the cell wall-based defense were found before inoculation. These results reveal that PAO is a nodal point in a specific apoplast-localized plant-pathogen interaction, which also signals parallel defense responses, thus preventing pathogen colonization. This strategy presents a novel approach for producing transgenic plants resistant to a broad spectrum of plant pathogens. 相似文献
32.
Skyrianou KC Perdih F Papadopoulos AN Turel I Kessissoglou DP Psomas G 《Journal of inorganic biochemistry》2011,105(10):1273-1285
The nickel(II) complexes with the quinolone antibacterial agents oxolinic acid, flumequine, enrofloxacin and sparfloxacin in the presence of the N,N′-donor heterocyclic ligand 2,2′-bipyridylamine have been synthesized and characterized. The quinolones act as bidentate ligands coordinated to Ni(II) ion through the pyridone oxygen and a carboxylato oxygen. The crystal structure of [(2,2′-bipyridylamine)bis(sparfloxacinato)nickel(II)] has been determined by X-ray crystallography. UV study of the interaction of the complexes with calf-thymus DNA (CT DNA) has shown that they bind to CT DNA with [(2,2′-bipyridylamine)bis(flumequinato)nickel(II)] exhibiting the highest binding constant to CT DNA. The cyclic voltammograms of the complexes have shown that in the presence of CT DNA the complexes can bind to CT DNA by the intercalative binding mode which has also been verified by DNA solution viscosity measurements. Competitive study with ethidium bromide (EB) has shown that the complexes can displace the DNA-bound EB indicating that they bind to DNA in strong competition with EB. The complexes exhibit good binding propensity to human or bovine serum albumin protein having relatively high binding constant values. The biological properties of the [Ni(quinolonato)2(2,2′-bipyridylamine)] complexes have been evaluated in comparison to the previously reported Ni(II) quinolone complexes [Ni(quinolonato)2(H2O)2], [Ni(quinolonato)2(2,2′-bipyridine)] and [Ni(quinolonato)2(1,10-phenanthroline)]. The quinolones and their Ni(II) complexes have been tested for their antioxidant and free radical scavenging activity. They have been also tested in vitro for their inhibitory activity against soybean lipoxygenase. 相似文献
33.
One-hundred and fifty different thermophilic bacteria isolated from a volcanic island were screened for detection of an alkane
hydroxylase gene using degenerated primers developed to amplify genes related to the Pseudomonas putida and Pseudomonas oleovorans alkane hydroxylases. Ten isolates carrying the alkJ gene were further characterized by 16s rDNA gene sequencing. Nine out of ten isolates were phylogenetically affiliated with
Geobacillus species and one isolate with Bacillus species. These isolates were able to grow in liquid cultures with crude oil as the sole carbon source and were found to degrade
long chain crude oil alkanes in a range between 46.64% and 87.68%. Results indicated that indigenous thermophilic hydrocarbon
degraders of Bacillus and Geobacillus species are of special significance as they could be efficiently used for bioremediation of oil-polluted soil and composting
processes. 相似文献
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35.
The selenophosphate synthetases from several organisms contain a selenocysteine residue in their active site where the Escherichia coli enzyme contains a cysteine. The synthesis of these enzymes, therefore, depends on their own reaction product. To analyse
how this self-dependence is correlated with the selenium status, e.g. after recovery from severe selenium starvation, we expressed
the gene for the selenocysteine-containing selenophosphate synthetase from Haemophilus influenzae (selD
HI) in an E. coliΔselD strain. Gene selD
HI gave rise to a selenium-containing gene product and also supported – via its activity – the formation of E. coli selenoproteins. The results provide evidence either for the suppression of the UGASec codon with the insertion of an amino acid allowing the formation of a functional product or for a bypass of the selenophosphate
requirement. We also show that the selenocysteine synthesis and the insertion systems of the two organisms are fully compatible
despite conspicuous differences in the mRNA recognition motif.
Received: 8 July 1997 / Accepted: 3 September 1997 相似文献
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37.
Georgia Kontostathi Jerome Zoidakis Nicholas P. Anagnou Kalliopi I. Pappa Antonia Vlahou 《Expert review of proteomics》2016,13(8):731-745
Introduction: The HPV virus accounts for the majority of cervical cancer cases. Although a diagnostic tool (Pap Test) is widely available, cervical cancer incidence still remains high worldwide, and especially in developing countries, attributed to a large extent to suboptimal sensitivities of the Pap test and unavailability of the test in developing countries.
Areas covered: Proteomics approaches have been used in order to understand the HPV virus correlation to cervical cancer pathology, as well as to discover putative biomarkers for early cervical cancer diagnosis and drug mode of action.
Expert commentary: The present review summarizes the latest in vitro and in vivo proteomic studies for the discovery of putative cervical cancer biomarkers and the evaluation of available drugs and treatments. 相似文献
38.
39.
Different metalated porphyrin compounds were studied as model complexes for cytochrome c oxidase. All models contain a tyrosine molecule and a copper binding site. Two of the compounds are bearing an axial pyridine ligand that could possibly coordinate with Fe porphyrins. All complexes were studied using NMR and UV-Vis spectroscopies and it was found that the coordination of the axial ligand is possible only in one of the porphyrins. Moreover, the synthesized catalysts were studied as promising enzyme mimics using a rotating disc electrode in the presence of molecular oxygen. 相似文献
40.
Multiphasic Approach To Study the Bacterial Ecology of Fermented Sausages Inoculated with a Commercial Starter Culture
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Luca Cocolin Rosalinda Urso Kalliopi Rantsiou Carlo Cantoni Giuseppe Comi 《Applied microbiology》2006,72(1):942-945
In this paper, the ability of a commercial starter culture to perform a sausage fermentation is evaluated. Molecular analysis revealed the presence of several strains of the same species contained in the starter culture with different behavior during the fermentation, and the contribution of Lactobacillus curvatus, which was only marginally isolated during the transformation. 相似文献