全文获取类型
收费全文 | 112篇 |
免费 | 14篇 |
出版年
2022年 | 1篇 |
2021年 | 3篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 4篇 |
2013年 | 7篇 |
2012年 | 7篇 |
2011年 | 10篇 |
2010年 | 3篇 |
2009年 | 2篇 |
2008年 | 4篇 |
2007年 | 5篇 |
2006年 | 3篇 |
2005年 | 2篇 |
2004年 | 2篇 |
2003年 | 1篇 |
2002年 | 1篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 3篇 |
1994年 | 1篇 |
1991年 | 4篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1971年 | 1篇 |
1970年 | 3篇 |
1969年 | 2篇 |
1966年 | 1篇 |
1965年 | 4篇 |
1964年 | 2篇 |
1963年 | 1篇 |
1959年 | 1篇 |
排序方式: 共有126条查询结果,搜索用时 15 毫秒
121.
122.
123.
Natsumi Maruta Yuri Trusov Daisuke Urano David Chakravorty Sarah M Assmann Alan M Jones Jose R Botella 《Plant physiology》2021,186(2):1240
The extra-large guanosine-5′-triphosphate (GTP)-binding protein 2, XLG2, is an unconventional Gα subunit of the Arabidopsis (Arabidopsis thaliana) heterotrimeric GTP-binding protein complex with a major role in plant defense. In vitro biochemical analyses and molecular dynamic simulations show that affinity of XLG2 for GTP is two orders of magnitude lower than that of the conventional Gα, AtGPA1. Here we tested the physiological relevance of GTP binding by XLG2. We generated an XLG2(T476N) variant with abolished GTP binding, as confirmed by in vitro GTPγS binding assay. Yeast three-hybrid, bimolecular fluorescence complementation, and split firefly-luciferase complementation assays revealed that the nucleotide-depleted XLG2(T476N) retained wild-type XLG2-like interactions with the Gβγ dimer and defense-related receptor-like kinases. Both wild-type and nucleotide-depleted XLG2(T476N) restored the defense responses against Fusarium oxysporum and Pseudomonas syringae compromised in the xlg2 xlg3 double mutant. Additionally, XLG2(T476N) was fully functional restoring stomatal density, root growth, and sensitivity to NaCl, but failed to complement impaired germination and vernalization-induced flowering. We conclude that XLG2 is able to function in a GTP-independent manner and discuss its possible mechanisms of action.Arabidopsis extra-large GTP-binding proteins have nucleotide-independent functions. 相似文献
124.
125.
The transport of α-methyl-D-glucoside and two aminoacids, L-phenylalanine and L-leucine by a temperature sensitive fatty acid
requiring mutant ofSalmonella typhimurium was studied under conditions of supplementation withcis or trans-unsaturated fatty acids. The results of such experiments definitely establish a relationship between the fatty acids
composition of the membrane and the transport property of the cells. Cells grown in the presence of trans-unsaturated fatty
acids cannot transport so efficiently as compared to the cis-unsaturated fatty acid-grown cells except linolelaidic acid,
atrans-trans-unsaturated fatty acid. Protein: phospholipid ratio of the membrane also varies significantly under such conditions. The
affinity of L-phenylalanine transport carrier for the substrate changes remarkably in cells grown in the presence of differentcis or trans-unsaturated fatty acids and indicate the possible role of membrane lipids in membrane assembly as well as regulation
of the activity of L-phenylalanine transport system. 相似文献
126.
Hala Zreiqat Ranita Sungaran C. Rolfe Howlett Boban Markovic 《Molecular biotechnology》1998,10(2):107-113
The universal quantitation of the DNA hybridization reaction has been a goal sought by many researchers. Part of this search
has been the need to develop a rapid, sensitive, easy-to-perform, and quantitative method to measure the abundance of specific
mRNAs directly within cells. Conventionally mRNA detection can be done by advanced quantitativein situ hybridization (ISH) using either image analysis or fluorescencein situ hybridization (FISH), or indirectly by extraction of mRNA from cells or tissue and using Northern blot or quantitative polymerase
chain reaction (PCR). We examined the quantitative nature of probe binding to intracellular mRNA in a sensitive and easy-to-use
nonisotopic method of ISH previously developed in our laboratories. The method is applicable to isolated primary cells or
cells in culture. The procedural details are very simple, with cells being centrifuged into 96-well microplates, fixed with
formalin, and pretreated with Triton X-100 and Nonidet P-40 before photobiotin-labeled cDNA probes are applied. Biotin from
the hybridization of probe to target is detected using multiple applications of streptavidin and biotinylated alkaline phosphatase
and visualized by thep-nitrophenyl phosphate conversion method. The quantitative parameters of the ISH procedure were determined by measuring the
levels of expression of erythropoietin (EPO) mRNA and its translated protein in transfected COS-7 cells. There is a log-linear
relationship between the levels of signal obtained in the ISH reaction in 96-well microplates and the EPO protein levels measured
by enzyme-linked immunosorbent assay (ELISA). This demonstrated relationship is important in the standardization and use of
these procedures to measure quantitatively mRNAs within cells. 相似文献