植物定植图微机显示与管理系统

介绍了运用软件工程的方法和数据库技术研究开发的南京中山植物园植物定植图微机显示与管理系统。详细介绍了系统的目标,系统的实现,应用软件的研制及应用效果。     

慢性缺氧对大鼠肺内皮素表达的影响

本文以ＡＢＣ法和原位杂交技术,观察了慢性缺氧时大鼠肺组织内内皮素－１（ＥＴ－１）的表达情况,结果发现：①正常肺血管内皮细胞有少许ＥＴ－１样阳性染色物质呈现。②缺氧ＩＷ后,肺内ＥＴ－１含量增加,主要位于肺血管内皮细胞和支气管粘膜上皮细胞。③缺氧２Ｗ和３Ｗ后,ＥＴ１阳性免疫物质进一步增加,于肺泡细胞内也见到阳性染色。④缺氧１Ｗ后肺内ＥＴ－１ｍＲＮＡ表达增加,缺氧２Ｗ和３Ｗ后,ＥＴ－１ｍＲＮＡ的表达进一步加强。提示缺氧可刺激肺内ＥＴ－１ｍＲＮＡ的表达,慢性缺氧时肺内ＥＴ－１持续分泌增加,这可能是缺氧性肺动脉高压发生的重要因素之一。     

海河流域14种农作物对土壤库中磷的输出量和输入量初步研究

 海河流域14种农作物平均含磷量为0.127±0.053%,变化范围0.023—0.214%,不同器官中最高可达0.419%,最低仅为0.019%。对土壤库中磷输出量最大者为谷子,磷可达99.758±56.931kg·ha-1·a-1,其次是玉米和棉花;磷输入量以白薯为最大,为12.557±5.020kg·ha-1·a-1(但包括可食部分块根的输出部分在内),然后是谷子和花生,其余作物均较低,<2kg·ha-1·a-1。具高输出量的作物的部位有玉米、谷子、花生、棉花等果实,谷子、花生、白薯等茎叶;就输入量而言,除白薯为高输入和谷子为低输入类型外,其余均属很低输入型,上述特点揭示了海河流域因作物的收获而大量损失土壤库中的磷,如种植谷子、玉米、棉花等分别以99.758、32.661和26.591kg·ha-1·a-1的速率损失有效态磷。并对不同子流域作物磷的输出(入)量差异以及针对上述问题应采取的对策作了探讨。     

离子交换法精制丙氨酸的研究

合成法生产的丙氨酸,产品中Ｃｌ￣－,含量过高,仅为工业级。我们采用多柱串联的离子交换系统,对工业级丙氨酸进行了脱ＣＬ￣－,脱试验,交换后丙氨酸产品中的Ｃｌ￣－、含量分别小于０．０２％和０．０３％,符合食品添加剂的标准。     

Epipolarization Microscopic Immunogold Assay: a Combination of Immunogold Silver Staining, Enzyme-Linked-Immunosorbent Assay and Epipolarization Microscopy

We describe a new immunoassay which combines an immunosorbent assay, Immunogold silver staining and epipolarization microscopy. Our new assay procedure features multiple samples on a single microscope slide, and high sensitivity of epipolarization microscope for detection of silver-enhanced colloidal gold as a final immunoassay product. We call the new immunoassay “on slide immunogold assay” (OSIGA). This new method uses biotinylated antibody and streptavidin-gold reaction with silver enhancement technique. With OSIGA it is possible to investigate 30 samples on a single microscopic slide. Our preliminary studies used 10-20 μ1 samples and detected nanogram quantities of a standardized protein solution. Unlike enzyme linked immunosorbent assay (ELISA), which has a limited time for reading the final color products, the OSIGA specimens can be dried or resin mounted for longer storage and future reference.     

噬菌蛭弧菌噬菌斑的鉴别与纯化的研究

本文报告了使用１／５００营养肉汤双层琼脂培养基,在含有宿主菌及寄生菌混合物的软琼脂层中,蛭弧菌、变形虫及鞭毛虫等均可形成噬菌斑,但其大小、形态、扩展速度不同。鉴别方法除依据噬菌斑形态特征外,主要靠相差显微镜下的形态学观察。蛭弧菌的纯化用单斑传代,一个直径为１ｍｍ的噬菌斑含有约１０^５－６ｐｆｕ／ｍｌ（每毫升噬菌斑形成单位）。     

一株能表达987P和F_(41)两种粘附素的猪源性大肠杆菌的研究

通过直接凝集试验、免疫荧光试验、SDS-PAGE和Western印迹,对一株猪源性大肠杆菌的粘附素进行了研究.结果表明,该菌株是一株同时表达987P和F_(41)两种粘附素抗原的猪源性大肠杆菌.     

Requirement of human immunodeficiency virus type 1 nef for in vivo replication and pathogenicity.

The role of human immunodeficiency virus type 1 (HIV-1) accessory genes in pathogenesis has remained unclear because of the lack of a suitable in vivo model. The most controversial of these genes is nef. We investigated the requirement for Nef for in vivo replication and pathogenicity of two isolates of HIV-1 (HIV-1JR-CSF and HIV-1NL4-3) in human fetal thymus and liver implants in severe combined immunodeficient mice. HIV-1JR-CSF and HIV-1NL4-3 differ in their in vitro phenotypes in that HIV-1JR-CSF does not induce syncytia and is relatively noncytopathic, while HIV-1NL4-3 is highly cytopathic and readily induces syncytia. The nef mutants of both isolates grew with kinetics similar to those of parental virus strains in stimulated peripheral blood lymphocytes but demonstrated attenuated growth properties in vivo. HIV-1NL4-3 induced severe depletion of human thymocytes within 6 weeks of infection, whereas its nef mutant did not. Thus, HIV-1 Nef is required for efficient in vivo viral replication and pathogenicity.     

Purification and characterization of recombinant human pro-urokinase produced in silkworm using a baculovirus vector

Summary A recombinant baculovirus (BmNPV-pk2) was constructed by inserting the human pro-urokinase(pro-UK) cDNA into the genome of baculovirus Bombyx mori nuclear polyhedrosis virus (BmNPV) adjacent to the strong polyhedrin promoter. The recombinant virus replicated in silkworm larvae, which synthesized 30g pro-UK/ml in the haemolymph within 4 days post-infection. Purification to near homogeneity was accomplished by fractional precipitation with ammonium sulphate and immunoaffinity chromatography with an overall yield of 23% and a specific activity of 100,000IU/mg in fibrin plate assay. This purified product was comprised of a single chain protein with approximately Mr. 50kDa as determined by SDS-PAGE gel. The N-Terminal amino acids sequence revealed that the secretion signal of pro-UK was correctly processed.     

小麦品种抗性对麦长管蚜种群增长的影响

本研究采用室内、外结合的方法,分苗期和拔节期,对大田抗蚜性鉴定中表现不同的10个小麦品种的抗性对麦长管蚜种群增长的影响进行了研究。研究结果显示,品种抗性对麦长管蚜的存活率、发育进度、繁殖力有一致的抑制作用;品种间抗蚜性存在显著差异。同时通过组建不同品种上麦长管蚜繁殖特征生命表,从量的角度揭示了小麦品种抗性与麦长管蚜种群增长之间的关系。