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41.
Objective
The nature of changes in brain activation related to good recovery of arm function after stroke is still unclear. While the notion that this is a reflection of neuronal plasticity has gained much support, confounding by compensatory strategies cannot be ruled out. We address this issue by comparing brain activity in recovered patients 6 months after stroke with healthy controls.Methods
We included 20 patients with upper limb paresis due to ischemic stroke and 15 controls. We measured brain activation during a finger flexion-extension task with functional MRI, and the relationship between brain activation and hand function. Patients exhibited various levels of recovery, but all were able to perform the task.Results
Comparison between patients and controls with voxel-wise whole-brain analysis failed to reveal significant differences in brain activation. Equally, a region of interest analysis constrained to the motor network to optimize statistical power, failed to yield any differences. Finally, no significant relationship between brain activation and hand function was found in patients. Patients and controls performed scanner task equally well.Conclusion
Brain activation and behavioral performance during finger flexion-extensions in (moderately) well recovered patients seems normal. The absence of significant differences in brain activity even in patients with a residual impairment may suggest that infarcts do not necessarily induce reorganization of motor function. While brain activity could be abnormal with higher task demands, this may also introduce performance confounds. It is thus still uncertain to what extent capacity for true neuronal repair after stroke exists. 相似文献42.
43.
Background and Aims
Animal pollination is typically an uncertain process that interacts with self-incompatibility status to determine reproductive success. Seed set is often pollen-limited, but species with late-acting self-incompatibility (SI) may be particularly vulnerable, if self-pollen deposition results in ovule discounting. Pollination is examined and the occurrence of late-acting SI and ovule discounting assessed in Cyrtanthus breviflorus.Methods
The pollination system was characterized by observing floral visitors and assessing nectar production and spectral reflectance of flowers. To assess late-acting SI and ovule discounting, growth of self- and cross-pollen tubes, and seed set following open pollination or hand pollination with varying proportions of self- and cross-pollen, were examined.Key Results
Native honeybees Apis mellifera scutellata pollinated flowers as they actively collected pollen. Most flowers (≥70 %) did not contain nectar, while the rest produced minute volumes of dilute nectar. The flowers which are yellow to humans are visually conspicuous to bees with a strong contrast between UV-reflecting tepals and UV-absorbing anthers and pollen. Plants were self-incompatible, but self-rejection was late-acting and both self- and cross-pollen tubes penetrated ovules. Seed set of open-pollinated flowers was pollen-limited, despite pollen deposition exceeding ovule number by 6-fold. Open-pollinated seed set was similar to that of the cross + self-pollen treatment, but was less than that of the cross-pollen-only treatment.Conclusions
Flowers of C. breviflorus are pollinated primarily by pollen-collecting bees and possess a late-acting SI system, previously unknown in this clade of the Amaryllidaceae. Pollinators of C. breviflorus deposit mixtures of cross- and self-pollen and, because SI is late-acting, self-pollen disables ovules, reducing female fertility. This study thus contributes to growing evidence that seed production in plants with late-acting SI systems is frequently limited by pollen quality, even when pollinators are abundant. 相似文献44.
Pollen commonly limits seed output in natural plant populations, but the relative contributions of pollen quantity and quality to pollen limitation remain largely unexplored. Estimates of these contributions are needed to promote better understanding of the ecological and evolutionary consequences of pollen limitation. We assessed pollen limitation and distinguished how pollen quantity and quality contributed to seed output in the predominantly outcrossing herb Bulbine bulbosa. We compared seed output of emasculated and cross-pollinated plants that could not self-pollinate with that of intact plants. Seed output was similar in emasculated and cross-pollinated plants, but it was about 50% less in intact plants. These findings indicate that the quantity of cross-pollen deposited by pollinators did not limit seed output. However, pollinators also deposited self-pollen, which reduced seed output because selfing disabled ovules, rendering them unavailable for cross-fertilization. We conclude that pollen quality rather than quantity can limit seed output under natural conditions, which supports recent proposals for greater consideration of pollen quality in the study of pollen limitation. 相似文献
45.
Mark S. Castro Keith N. Eshleman Louis F. Pitelka Geoff Frech Molly Ramsey William S. Currie Karen Kuers Jeffrey A. Simmons Bob R. Pohlad Carolyn L. Thomas David M. Johnson 《Biogeochemistry》2007,84(3):333-348
The objective of this study was to evaluate the nitrogen (N) biogeochemistry of an 18–22 year old forested watershed in western
Maryland. We hypothesized that this watershed should not exhibit symptoms of N saturation. This watershed was a strong source
of nitrate (NO3
−) to the stream in all years, with a mean annual export of 9.5 kg N ha−1 year−1 and a range of 4.4–18.4 kg N ha−1 year−1. During the 2001 and 2002 water years, wet deposition of inorganic N was 9.0 kg N ha−1 year−1 and 6.3 kg N ha−1 year−1, respectively. Watershed N export rates in 2001 and 2002 water years were 4.2 kg N ha−1 year−1 and 5.3 kg N ha−1 year−1, respectively. During the wetter water years of 2003 and 2004, the watershed exported 15.0 kg N ha−1 year−1 and 18.4 kg N ha−1 year−1, rates that exceeded annual wet deposition of N by a factor of two (7.5 kg N ha−1 year−1 in 2003) and three (5.5 kg N ha−1 year−1 in 2004). Consistent with the high rates of N export, were high concentrations (2.1–3.3%) of N in foliage, wood (0.3%) and
fine roots, low C:N ratios in the forest floor (17–24) and mineral soil (14), high percentages (83–96%) of the amount of mineralized
N that was nitrified and elevated N concentrations (up to 3 mg N l−1) in soil solution. Although this watershed contained a young aggrading forest, it exhibited several symptoms of N saturation
commonly observed in more mature forests. 相似文献
46.
Herbivores in nutrient‐limited systems such as arctic tundra have been suggested to play a minor role in controlling plant growth simply because they are relatively few in number. However, theory predicts that as net primary productivity (NPP) increases because of greater inputs of nutrients or energy, herbivores may have greater effects on plant growth. This prediction has not been tested in the context of climate warming in arctic tundra, which may increase soil nutrient availability and thus NPP. We examined a long‐term experiment that excluded small and large mammalian herbivores and increased soil nutrients in two arctic Alaskan tundra communities: dry heath (DH) and moist acidic tussock (MAT). In the ninth year of manipulations, we measured weekly growth of three plant species of three growth forms: tussock‐forming graminoid, rhizomatous graminoid, and dwarf deciduous shrub, in each community. All species grew better when fertilized. In DH, this increase in growth was exaggerated when plants were protected from herbivores, confirming that herbivory had a negative effect on plant growth under increased nutrient conditions, but was unimportant under ambient soil conditions. However, in MAT, the importance of herbivory differed among species with fertilization. The tussock‐forming sedge at MAT, Eriophorum vaginatum, grew better and flowered more when fenced under both ambient and amended nutrients compared to plants exposed to herbivores. This species decreases in abundance in long‐term fertilized plots when mammals are present, and our results suggest that herbivory may be accounting for at least some of that loss, in addition to shifts in competitive relationships. Although we only focused on individual plants here rather than the entire community, our results suggest that under the increased soil nutrient conditions expected with continued climate warming in the Arctic, herbivores may become more important in affecting several abundant tundra plant populations, and should not be ignored. 相似文献
47.
48.
49.
Knapp AM Ramsey JE Wang SX Strauch AR Kelm RJ 《The Journal of biological chemistry》2007,282(49):35899-35909
50.
Evaluation of the vervet (Clorocebus aethiops) as a model for the assisted reproductive technologies
Sparman ML Ramsey CM Thomas CM Mitalipov SM Fanton JW Maginnis GM Stouffer RL Wolf DP 《American journal of primatology》2007,69(8):917-929
The vervet monkey was evaluated as a primate model for use in assisted reproductive technologies (ARTs). Eight adult female vervets were hormonally monitored for their potential use as egg donors and those six females displaying regular menstrual cycles were subjected to controlled ovarian stimulation with recombinant human gonadotropins. Three animals failed to respond while laparoscopic follicular aspiration was performed on the other three females at 27-30 h post-human chorionic gonadotropin administration. A total of 62, 40, and 18 oocytes was recovered from these three animals of which 30, 20, and 4, respectively, matured to the metaphase II stage and were subsequently inseminated using intracytoplasmic sperm injection. An average of 40+/-15% (SEM) of the inseminated oocytes were fertilized based on pronucleus formation and timely cleavage. One embryo from each of the two stimulated females developed into expanded blastocysts. Two adult male vervets were assessed as sperm donors. Neither adjusted well to the restraint and collection procedure required for penile electroejaculation. Samples collected via rectal electroejaculation were very low in sperm motility and concentration; however, cauda epididymal aspirations from one male yielded an adequate concentration of motile sperm. These results emphasize the need to establish species-specific ovarian stimulation protocols and semen collection techniques if vervets are to be considered for basic and applied (ARTs) research on primate gametes or embryos. 相似文献