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51.
52.
Gil Rodas Mar Calvo Asunción Estruch Eduardo Garrido Guadalupe Ercilla Antoni Arcas Ramon Segura Josep L. Ventura 《European journal of applied physiology and occupational physiology》1998,77(6):511-516
Running economy (RE), defined as the steady-state of oxygen uptake (V˙O2) for a given running velocity, is a factor of sports performance the genetic component of which has seldom been reported
to date. We studied this component using a heritability index (HI) in a group of 32 male twins, 8 monozygotic (MZ) and 8 dizygotic
(DZ) pairs, all sportsmen with similar perinatal and environmental backgrounds. Zygocity was determined by the identity of
erythrocytic antigenic, protein and enzymatic polymorphism, and human leucocyte antigen serologic types between co-twins.
The subjects exercised twice on a treadmill, once until exhaustion and again at submaximal intensities. Pulmonary gas exchange
was measured continuously using an automatic analyser system during both tests. Blood samples were obtained during the recovery
period to determine lactate concentrations. No significant differences were observed between MZ and DZ, in respect of RE at
any speed or in maximal V˙O2 relative to body mass. Nevertheless, significant HI (P < 0.05) was found in maximal lactate concentrations (HI = 0.75) and in respiratory equivalent for oxygen at two speeds, 7 km · h−1 (HI = 0.71) and 8 km · h−1 (HI = 0.79), differences which probably suggest that there are differences in RE. In conclusion, we did not detect a genetic
component in RE or in maximal oxygen uptake, but a genetic component for markers of anaerobic metabolism was present.
Accepted: 5 November 1997 相似文献
53.
Mª Dolores Ramos-Barbero Tomeu Viver Ane Zabaleta Ece Senel María Gomariz Iñaki Antigüedad Fernando Santos Manuel Martínez-García Ramon Rosselló-Móra Josefa Antón 《Environmental microbiology》2021,23(7):3477-3498
Microbial communities in hypersaline underground waters derive from ancient organisms trapped within the evaporitic salt crystals and are part of the poorly known subterranean biosphere. Here, we characterized the viral and prokaryotic assemblages present in the hypersaline springs that dissolve Triassic-Keuper evaporite rocks and feed the Añana Salt Valley (Araba/Alava, Basque Country, Spain). Four underground water samples (around 23% total salinity) with different levels of exposure to the open air were analysed by means of microscopy and metagenomics. Cells and viruses in the spring water had lower concentrations than what are normally found in hypersaline environments and seemed to be mostly inactive. Upon exposure to the open air, there was an increase in activity of both cells and viruses as well as a selection of phylotypes. The underground water was inhabited by a rich community harbouring a diverse set of genes coding for retinal binding proteins. A total of 35 viral contigs from 15 to 104 kb, representing partial or total viral genomes, were assembled and their evolutionary changes through the spring system were followed by SNP analysis and metagenomic island tracking. Overall, both the viral and the prokaryotic assemblages changed quickly upon exposure to the open air conditions. 相似文献
54.
Murphy D Doyle H Eritja R Redmond G 《Journal of biomolecular structure & dynamics》2004,22(2):195-204
Three-strand oligonucleotide complexes are employed to assess the effect of base stacking and base pair mismatch on the relative thermodynamic stabilities of oligonucleotide duplexes. The melting behavior of three-strand oligonucleotide complexes incorporating nicks and gaps as well as internal single base mismatches is monitored using temperature-dependent optical absorption spectroscopy. A sequential three-state equilibrium model is used to analyze the measured melting profiles and evaluate thermodynamic parameters associated with dissociation of the complexes. The free-energy of stabilization of a nick complex compared to a gap complex due to base stacking is determined to be -1.9 kcal/mol. The influence of a mispaired base in these systems is shown to destabilize a nick complex by 3.1 kcal/mol and a gap complex by 2.8 kcal/mol, respectively. 相似文献
55.
Ramon HE Beal AM Liu Y Worthen GS Oliver PM 《Journal of immunology (Baltimore, Md. : 1950)》2012,188(8):4023-4031
Ndfip1 is an adaptor for the E3 ubiquitin ligase Itch. Both Ndfip1- and Itch-deficient T cells are biased toward Th2 cytokine production. In this study, we demonstrate that lungs from Ndfip1(-/-) mice showed increased numbers of neutrophils and Th17 cells. This was not because Ndfip1(-/-) T cells are biased toward Th17 differentiation. In fact, fewer Ndfip1(-/-) T cells differentiated into Th17 cells in vitro due to high IL-4 production. Rather, Th17 differentiation was increased in Ndfip1(-/-) mice due to increased numbers of IL-6-producing eosinophils. IL-6 levels in mice that lacked both Ndfip1 and IL-4 were similar to wild-type controls, and these mice had fewer Th17 cells in their lungs. These results indicate that Th2 inflammation, such as that observed in Ndfip1(-/-) mice, can increase Th17 differentiation by recruiting IL-6-producing eosinophils into secondary lymphoid organs and tissues. This may explain why Th17 cells develop within an ongoing Th2 inflammatory response. 相似文献
56.
Ramiro Logares Stephane Audic Sebastien Santini Massimo C Pernice Colomban de Vargas Ramon Massana 《The ISME journal》2012,6(10):1823-1833
Flagellated heterotrophic microeukaryotes have key roles for the functioning of marine ecosystems as they channel large amounts of organic carbon to the upper trophic levels and control the population sizes of bacteria and archaea. Still, we know very little on the diversity patterns of most groups constituting this evolutionary heterogeneous assemblage. Here, we investigate 11 groups of uncultured flagellates known as MArine STramenopiles (MASTs). MASTs are ecologically very important and branch at the base of stramenopiles. We explored the diversity patterns of MASTs using pyrosequencing (18S rDNA) in coastal European waters. We found that MAST groups range from highly to lowly diversified. Pyrosequencing (hereafter ‘454'') allowed us to approach to the limits of taxonomic diversity for all MAST groups, which varied in one order of magnitude (tens to hundreds) in terms of operational taxonomic units (98% similarity). We did not evidence large differences in activity, as indicated by ratios of DNA:RNA-reads. Most groups were strictly planktonic, although we found some groups that were active in sediments and even in anoxic waters. The proportion of reads per size fraction indicated that most groups were composed of very small cells (∼2–5 μm). In addition, phylogenetically different assemblages appeared to be present in different size fractions, depths and geographic zones. Thus, MAST diversity seems to be highly partitioned in spatial scales. Altogether, our results shed light on these ecologically very important but poorly known groups of uncultured marine flagellates. 相似文献
57.
Shanna Bitencourt Fernanda Mesquita Bruno Basso Júlia Schmid Gabriela Ferreira Lucas Rizzo Moises Bauer Ramon Bartrons Francesc Ventura Jose Luis Rosa Inge Mannaerts Leo Adrianus van Grunsven Jarbas Oliveira 《Cell biochemistry and biophysics》2014,68(2):387-396
Capsaicin, the active component of chili pepper, has been reported to have antiproliferative and anti-inflammatory effects on a variety of cell lines. In the current study, we aimed to investigate the effects of capsaicin during HSC activation and maintenance. Activated and freshly isolated HSCs were treated with capsaicin. Proliferation was measured by incorporation of EdU. Cell cycle arrest and apoptosis were investigated using flow cytometry. The migratory response to chemotactic stimuli was evaluated by a modified Boyden chamber assay. Activation markers and inflammatory cytokines were determined by qPCR, immunocytochemistry, and flow cytometry. Our results show that capsaicin reduces HSC proliferation, migration, and expression of profibrogenic markers of activated and primary mouse HSCs. In conclusion, the present study shows that capsaicin modulates proliferation, migration, and activation of HSC in vitro. 相似文献
58.
Eneko Axpe Tamara Lopez-Euba Ainara Castellanos-Rubio David Merida Jose Angel Garcia Leticia Plaza-Izurieta Nora Fernandez-Jimenez Fernando Plazaola Jose Ramon Bilbao 《PloS one》2014,9(1)
Positron annihilation lifetime spectroscopy (PALS) provides a direct measurement of the free volume void sizes in polymers and biological systems. This free volume is critical in explaining and understanding physical and mechanical properties of polymers. Moreover, PALS has been recently proposed as a potential tool in detecting cancer at early stages, probing the differences in the subnanometer scale free volume voids between cancerous/healthy skin samples of the same patient. Despite several investigations on free volume in complex cancerous tissues, no positron annihilation studies of living cancer cell cultures have been reported. We demonstrate that PALS can be applied to the study in human living 3D cell cultures. The technique is also capable to detect atomic scale changes in the size of the free volume voids due to the biological responses to TGF-β. PALS may be developed to characterize the effect of different culture conditions in the free volume voids of cells grown in vitro. 相似文献
59.
Mauricio Vergara Silvana Becerra Julio Berrios Nelson Osses Juan Reyes María Rodríguez-Moyá Ramon Gonzalez Claudia Altamirano 《PloS one》2014,9(4)
Mild hypothermia condition in mammalian cell culture technology has been one of the main focuses of research for the development of breeding strategies to maximize productivity of these production systems. Despite the large number of studies that show positive effects of mild hypothermia on specific productivity of r-proteins, no experimental approach has addressed the indirect effect of lower temperatures on specific cell growth rate, nor how this condition possibly affects less specific productivity of r-proteins. To separately analyze the effects of mild hypothermia and specific growth rate on CHO cell metabolism and recombinant human tissue plasminogen activator productivity as a model system, high dilution rate (0.017 h−1) and low dilution rate (0.012 h−1) at two cultivation temperatures (37 and 33°C) were evaluated using chemostat culture. The results showed a positive effect on the specific productivity of r-protein with decreasing specific growth rate at 33°C. Differential effect was achieved by mild hypothermia on the specific productivity of r-protein, contrary to the evidence reported in batch culture. Interestingly, reduction of metabolism could not be associated with a decrease in culture temperature, but rather with a decrease in specific growth rate. 相似文献
60.
Enrique Ramon Moliner 《Biotechnic & histochemistry》1957,32(3):105-116
Pieces of fresh nervous tissue 3-5 mm thick are put into a mixture of: 6% K2Cr2O7, 40 ml; 5% KClO3, 20 ml; 20% chloral hydrate, 30 ml; and concentrated formalin (38% HCHO), 10 ml; allowed to fix 3 days, with a daily change of fluid; transferred to 3% K2Cr2O7 for 3 days, with twice daily changes; then to 1% AgNO3 for 3 days at 20-25° C. Frozen sections are cut, dehydrated, cleared and mounted in Permount with a cover glass. The method gives good results for microglia and oligodendroglia in addition to the usual staining of nerve cells and their processes. 相似文献