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371.
Summary A spectrophotometric assay for cephalosporin C was established usingAlcaligenes faecalis as a test organism. The optimal pH range for the sensitivity of the assay was pH 6.7–7.4. The dose-response curve became linear after 4 h incubation with 15% (v/v) dosage. Deviations between spectrophotometric and chemical assays were within 6%. The cost of the spectrophotometric assay is about one-hundredth that of the chemical assay. 相似文献
372.
Nagalapura Ramakrishnappa Prasannakumar Vala Keshava Rao Nagendraiah Jyothi Sarikonda Saroja Ankanahalli Naranayanashetty Lokesha Govindaraju Ramkumar 《Journal of Applied Entomology》2023,147(2):105-115
Aphids, thrips, whiteflies, mealy bugs and mites could cause significant yield losses in horticultural crops. Many synthetic chemicals highly hazardous to non-target organisms and the environment are in use in their management. Botanicals are ideal for safe sustainable pest management by keeping synthetic chemical use at a minimum. We studied the insecticidal properties of three botanicals viz., Annona squamosa, Ricinus communis and Sapindus mukorossi seed extracts against sucking pests viz., aphids (Aphis gossypii and Aphis craccivora) on okra (Abelmoschus esculentus) and bittergourd (Momordica charantia), thrips (Scirtothrips dorsalis) on chilli (Capsicum frutescens), whiteflies (Bemisia tabaci) on eggplant (Solanum melongena), mealy bugs (Maconellicoccus hirsutus) on betel vine (Piper betle) and mites (Tetranychus urticae) on eggplant (Solanum melongena) under laboratory conditions. The bioassay showed that A. squamosa seed extract has potent insecticidal properties on all sucking pests, except T. urticae. Though S. mukorossi seed extract exhibited no insecticidal property, had a significant acaricidal property. A. squamosa and S. mukorossi seed extracts will therefore be additional botanicals for use in organic farming for managing the sucking pests effectively. 相似文献
373.
Selvan Nehru John Abraham Anitha Priya Sekar Hariharan Rajadurai Vijay Solomon Selvakumar Veeralakshmi 《Journal of biomolecular structure & dynamics》2020,38(7):2057-2067
AbstractFor efficient designing of metallodrugs, it is imperative to analyse the binding affinity of those drugs with drug-carrying serum albumins to comprehend their structure–activity correlation for biomedical applications. Here, cobalt(II) and cobalt(III) complexes comprising three phendione ligands, [Co(phendione)3]Cl2 (1) and [Co(phendione)3]Cl3 (2), where, phendione = 1,10-phenanthroline-5,6-dione, has been chosen to contrast the impact of their hydrophobicity and ionicity on binding with bovine serum albumin (BSA) through spectrophotometric titrations. The attained hydrophobicity values using octanol/water partition coefficient method manifested that complex 1 is more hydrophobic than complex 2, which could be attributed to lesser charge on its coordination sphere. The interaction of complexes 1 and 2 with BSA using steady state fluorescence studies revealed that these complexes quench the intrinsic fluorescence of BSA through static mechanism, and the extent of quenching and binding parameters are higher for complex 2. Further thermodynamics of BSA-binding studies revealed that complexes 1 and 2 interact with BSA through hydrophobic and hydrogen bonding/van der Waals interactions, respectively. Further, UV–visible absorption, circular dichroism and synchronous fluorescence studies confirmed the occurrence of conformational and microenvironmental changes in BSA upon binding with complexes 1 and 2. Molecular docking studies have also shown that complex 2 has a higher binding affinity towards BSA as compared to complex 1. This sort of modification of ionicity and hydrophobicity of metal complexes for getting desirable binding mode/strength with drug transporting serum albumins will be a promising pathway for designing active and new kind of metallodrugs for various biomedical applications.Communicated by Ramaswamy H. Sarma 相似文献
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376.
Priya Hariharan Madhavi Sawant Manju Gorivale Ruma Manchanda Roshan Colah K. Ghosh Anita Nadkarni 《Molecular biology reports》2017,44(5):413-417
Co-inheritance of gamma and beta globin gene mutations in a compound heterozygous state is rare but of clinical interest as it provides an important data on understanding the HbF expression. Hematological analysis was carried out (Sysmex KX-21). F-cells were enumerated using flow cytometry. Beta globin gene was analysed by CRDB technique and by DNA sequencing. Gamma globin promoter region was sequenced and expression studies were carried out using real time Taqman assay. We report a family, where two inherited defects of the β globin gene cluster segregate. The proband and her sibling were compound heterozygotes for a novel Gγ promoter mutation and the 619 bp deletion a common Indian β thalassemia mutation. Molecular characterization revealed that the father (HbA2 5.1%, HbF 5.4%), proband (HbA2 3.6%, HbF 31.7%) and her brother (HbA2 3.9%, HbF 23.6%) were heterozygous for the 619 bp deletion. The mother (HbA2 2.1%, HbF 3.4%) had a normal β globin gene. As both the children showed high HbF levels, the γ globin gene work up was carried out. The Gγ-globin gene promoter analysis revealed that the mother and the two children were heterozygous for a 5 bp deletion -ATAAG (-533 to -529) that resides in the GATA binding site. These findings suggest that the 5 bp deletion in the Gγ globin promoter has a functional role in silencing the γ-globin gene expression in adults by disrupting GATA-1 binding and the associated repressor complex and results in the up-regulation of gamma globin gene expression. When co-inherited with β -thalassemia trait it leads to a phenotype of HPFH. 相似文献
377.
Plasmonics - This study presents an in situ growth technique to develop highly sensitive plasmonic fiber optic sensors with an excellent control over the plasmonic properties of gold (AuNPs) and... 相似文献