全文获取类型
收费全文 | 3085篇 |
免费 | 331篇 |
国内免费 | 1篇 |
专业分类
3417篇 |
出版年
2023年 | 23篇 |
2022年 | 54篇 |
2021年 | 96篇 |
2020年 | 74篇 |
2019年 | 79篇 |
2018年 | 91篇 |
2017年 | 81篇 |
2016年 | 139篇 |
2015年 | 162篇 |
2014年 | 171篇 |
2013年 | 200篇 |
2012年 | 221篇 |
2011年 | 212篇 |
2010年 | 145篇 |
2009年 | 115篇 |
2008年 | 142篇 |
2007年 | 153篇 |
2006年 | 139篇 |
2005年 | 117篇 |
2004年 | 131篇 |
2003年 | 87篇 |
2002年 | 83篇 |
2001年 | 62篇 |
2000年 | 69篇 |
1999年 | 76篇 |
1998年 | 39篇 |
1997年 | 23篇 |
1996年 | 26篇 |
1995年 | 19篇 |
1994年 | 14篇 |
1993年 | 23篇 |
1992年 | 28篇 |
1991年 | 26篇 |
1990年 | 34篇 |
1989年 | 28篇 |
1988年 | 23篇 |
1987年 | 30篇 |
1986年 | 17篇 |
1985年 | 21篇 |
1984年 | 21篇 |
1983年 | 18篇 |
1982年 | 9篇 |
1980年 | 6篇 |
1979年 | 7篇 |
1978年 | 10篇 |
1976年 | 6篇 |
1975年 | 12篇 |
1971年 | 7篇 |
1970年 | 6篇 |
1969年 | 9篇 |
排序方式: 共有3417条查询结果,搜索用时 15 毫秒
121.
Increased fire frequency can significantly erode both soil properties and plant–pollinator interactions affecting plant reproductive success but they have seldom been assessed simultaneously. Here, we evaluate soil properties, pollinator assemblage and the reproductive success of two native Fabaceae herbs, Desmodium uncinatum and Rhynchosia edulis, growing in unburned, low and high fire frequency sites of Chaco Serrano across two consecutive years. Desmodium uncinatum is outcrossing with a high dependence on pollinators, whereas R. edulis is autogamous and completely independent of pollinators. We found that soil water content, nitrates and electrical conductivity significantly decreased in low and high fire frequency sites. Pollinator richness and composition visiting each plant species was similar across all fire frequency scenarios. However, fruit set of the exogamous D. uncinatum was strongly reduced in frequently burned sites, whereas fruit set of the autogamous R. edulis showed no significant changes. In both species, the probability of setting fruits was positively related to soil quality across fire frequency scenarios, implying that decreased reproduction was mainly driven by limitation of abiotic resources shaped by increased fire frequency. Because the pollinator-dependent D. uncinatum has a higher reproductive cost, reduced soil quality induced by fire frequency had stronger effects on its reproduction. Chronic reduction of sexual reproduction in frequently burned sites with depleted soils will limit population recruitment with negative consequences on long-term plant population persistence. 相似文献
122.
Seed predation and seedling recruitment in plants: the effect of the distance between parents 总被引:2,自引:0,他引:2
We present a graphic model that explores the effect of distance between parent plants on seed predation and seedling recruitment. Based on the assumption that distance between parents may affect the shape of the seed shadow, the model predicts that seed predators may affect seedling recruitment curves under isolated plants but they are unable to affect these curves under close parent plants. The predictions of the model are tested experimentally in Cryptocarya alba (Lauraceae), a common tree of the Mediterranean forest, Central Chile. Results show that predictions are not met under isolated parent plants. Although seed density decreases significantly away from parent plants, this effect is not relevant for seed predation and seedling recruitment. The biotic/abiotic contrast existing under the canopy vs outside the canopy, plus the shade-tolerance of this tree, better explains the seedling recruitment observed under isolated parent plants. Nevertheless, the predictions of the model are corroborated under close parent plants. Seed shadows overlap to the extent that they generate a homogeneous seed distribution, homogeneous seed predation and homogeneous seedling recruitment as well. We discuss the implications of the model in terms of the spatial pattern of seedlings and the benefits of dispersal from isolated and close parent plants. 相似文献
123.
The native conformation of antithrombin III (ATIII) is a poor inhibitor of its coagulation pathway target enzymes because of the partial insertion of its reactive center loop (RCL) in its central A beta-sheet. This study focused on tyrosine 131, which is located at the helix D-sheet A interface, adjacent to the ATIII pentasaccharide and heparin cofactor-binding sites and some 17A away from the RCL insertion. Crystallographic structures show that the Tyr(131) ring is buried in native ATIII and then becomes exposed when pentasaccharide binds to the inhibitor and activates it. This change suggested that Tyr(131) might serve as a switch for ATIII conformational activation. The hypothesis is supported by results from this study, which progressively removed atoms from the Tyr(131) side chain. Rates of heparin-independent Y131L and Y131A factor Xa inhibition were 25 and 29 times faster than for the control and Y131F, suggesting that Tyr(131) ring interactions with neighboring helix D and strand 2A residues shift the uncatalyzed native-to-activated conformational equilibrium toward the RCL-inserted state. Thermal denaturation experiments showed Y131A and Y131L were less stable than the control and Y131F, implying an increased tendency toward A-sheet mobility in these genetically activated molecules. Thus, the tight Tyr(131)-Asn(127)-Leu(130)-Leu(140)-Ser(142) cluster at the helix D-strand 2A interface of native antithrombin contributes significantly to the stability of the ground state conformation, and tyrosine 131 serves as a heparin-responsive molecular switch during the allosteric activation of ATIII anticoagulant activity. 相似文献
124.
RhoA activation promotes transformation and loss of thyroid cell differentiation interfering with thyroid transcription factor-1 activity. 总被引:1,自引:0,他引:1
125.
Methyl-CpG binding proteins identify novel sites of epigenetic inactivation in human cancer 总被引:18,自引:0,他引:18
Ballestar E Paz MF Valle L Wei S Fraga MF Espada J Cigudosa JC Huang TH Esteller M 《The EMBO journal》2003,22(23):6335-6345
126.
Hilda E. Garay Olivia Niebla Luis J. González Tamara Menéndez Luis J. Cruz Osvaldo Reyes 《Letters in Peptide Science》2000,7(2):97-105
Two peptides derived from the surface loop 4 of class1 Outer Membrane Protein (OMP) of Neisseriameningitidis were synthesized on solid phase usingthe Boc/Bzl strategy: one containing the entire loop4 cyclized and the other representing the polymerizedcyclic loop 4. To test a more efficient cyclic peptidepresentation, in the present study astrategy was developed to obtain polymers of cyclic peptides. Inorder to obtain the polymeric cyclic peptide, twoprotecting groups for cysteine were used – Acm andMob. The Cys(Acm)-protected cyclic peptide wasobtained after removing the Mob group. Thepolymerization reaction was carried out bysimultaneous deprotection/oxidation of S-Acmwith iodine. Analysis of the polymeric cyclic peptidein Tris-tricine-SDS-PAGE showed different bandswith molecular weights higher than expected for thecorresponding monomeric cyclic peptide. Both peptideswere used in immunization of four different mouse strains.The antisera raised against the peptides wereevaluated by ELISA and Western blotting vs. OMPpreparation of N. meningitidis. The titersraised against the polymerized cyclic peptide werehigher than the ones raised against the cyclicpeptide. The antisera elicited did not showbactericidal activity. Nevertheless, the antiseraelicited against the polymeric cyclic peptide in theCBA/J mouse strain showed opsonic activity. Theantibodies raised against the polymeric cyclic peptidewere successfully used as probes in Western blottingexperiments to verify the display of loop 4 peptide onthe surface of filamentous phage M13. 相似文献
127.
Mari Cruz Díaz-Barradas María Zunzunegui Fatima Ain-Lhout Juan Jáuregui Said Boutaleb Leonor Álvarez-Cansino Mari Paz Esquivias 《Plant and Soil》2010,337(1-2):217-231
Argania spinosa (the argan tree) is a slow-growing tree endemic of Morocco, growing on semi-arid areas where no other tree species can live. With the aim of predicting temporal changes in A. spinosa woodlands under a probable increase in aridity, we set off to investigate these questions: how do A. spinosa physiological attributes respond to variations in climatic conditions and seasonality, and which is the set of attributes that most affects tree response to environmental conditions? In three study sites, Beni Snassen (North), High-Atlas (Mountain) and Admine Forest in Agadir (Coastal), gas exchange measurements, photochemical efficiency, leaf water potential and different leaf attributes were monitored in February, July and November of 2006. The Mountain site presents the most continental climate. Trees in this site were the most stressed in summer, having the lowest midday leaf water potential values, photochemical efficiency and assimilation rates. We found a Ψmd threshold around -4 MPa, below which stomatal conductance responds linearly to Ψmd. Plants from the North area never reached this threshold during the study period. Although leaf pigments presented a clear seasonal pattern, leaves from Coastal trees exhibit the highest content for each season. The three study sites were separated by two discriminate functions obtained by canonical discriminant analysis. In summer, the Mountain population is separated from the other sites mainly by assimilation rate and Fv/Fm, while in winter transpiration rates and chlorophyll content are the main discriminant variables. Our study shows that A. spinosa trees adjust their physiological status and leaf attributes to environmental conditions allowing plants to thrive under a dry climate. Under a scenario of global change, the distribution of the argan tree likely shifts to milder areas. 相似文献
128.
129.
Maria A. Ledesma Sara A. Ochoa Ariadnna Cruz Luz M. Rocha-Ramírez Jaime Mas-Oliva Carlos A. Eslava Jorge A. Girón Juan Xicohtencatl-Cortes 《PloS one》2010,5(8)
Background
Enterohemorrhagic Escherichia coli (EHEC) O157:H7, the causative agent of hemorrhagic colitis and the hemolytic uremic syndrome (HUS), produces long bundles of type IV pili (TFP) called hemorrhagic coli pili (HCP). HCP are capable of mediating several phenomena associated with pathogenicity: i) adherence to human and bovine epithelial cells; ii) invasion of epithelial cells; iii) hemagglutination of rabbit erythrocytes; iv) biofilm formation; v) twitching motility; and vi) specific binding to laminin and fibronectin. HCP are composed of a 19 kDa pilin subunit (HcpA) encoded by the hcpA chromosomal gene (called prepilin peptidase-dependent gene [ppdD] in E. coli K-12).Methodology/Principal Findings
In this study we investigated the potential role of HCP of E. coli O157:H7 strain EDL933 in activating the release of pro- and anti-inflammatory cytokines from a variety of host epithelial cells. We found that purified HCP and a recombinant HcpA protein induced significant release of IL-8 and TNF-α, from cultured polarized intestinal cells (T84 and HT-29 cells) and non-intestinal HeLa cells. Levels of proinflammatory IL-8 and TNF-α, but not IL-2, IL6, or IL-10 cytokines, were increased in the presence of HCP and recombinant HcpA after 6 h of incubation with ≥50 ng/ml of protein, suggesting that stimulation of IL-8 and TNF-α are dose and time-dependent. In addition, we also demonstrated that flagella are potent inducers of cytokine production. Furthermore, MAPK activation kinetics studies showed that EHEC induces p38 phosphorylation under HCP-producing conditions, and ERK1/2 and JNK activation was detectable after 3 h of EHEC infection. HT-29 cells were stimulated with epidermal growth factor stimulation of HT-29 cells for 30 min leading to activation of three MAPKs.Conclusions/Significance
The HcpA pilin monomer of the HCP produced by EHEC O157:H7 is a potent inducer of IL-8 and TNF-α release, an event which could play a significant role in the pathogenesis of hemorrhagic colitis caused by this pathogen. 相似文献130.
Luis Martínez-Gil Jesús A. Sánchez-Navarro Antonio Cruz Vicente Pallás Jesús Pérez-Gil Ismael Mingarro 《Journal of virology》2009,83(11):5535-5543
The cell-to-cell transport of plant viruses depends on one or more virus-encoded movement proteins (MPs). Some MPs are integral membrane proteins that interact with the membrane of the endoplasmic reticulum, but a detailed understanding of the interaction between MPs and biological membranes has been lacking. The cell-to-cell movement of the Prunus necrotic ringspot virus (PNRSV) is facilitated by a single MP of the 30K superfamily. Here, using a myriad of biochemical and biophysical approaches, we show that the PNRSV MP contains only one hydrophobic region (HR) that interacts with the membrane interface, as opposed to being a transmembrane protein. We also show that a proline residue located in the middle of the HR constrains the structural conformation of this region at the membrane interface, and its replacement precludes virus movement.Plant viruses encode movement proteins (MPs) that mediate the intra- and intercellular spread of the viral genome via plasmodesmata, membranous channels that traverse the walls of plant cells and enable intercellular transport and communication. There is a range of diversity in the number and type of viral proteins required for viral movement (21). Research on tobacco mosaic virus (TMV) has played a leading role in understanding MP activity (2). The genome of TMV encodes a single 30-kDa multidomain protein, the namesake of the 30K superfamily (7). Viral RNA is associated with the membrane of the endoplasmic reticulum (ER) and microtubules in the presence of this MP (23, 30).A large number of plant viruses have 30K MPs, which share common abilities, including binding nucleic acids, localizing and increasing the size exclusion limit of plasmodesmata, and interacting with the ER membrane. A topological model has been proposed in which the TMV MP has two putative transmembrane (TM) helices, both the N and C termini oriented toward the cytoplasm, and a short loop exposed in the ER lumen (4). There is less experimental information for other 30K MPs, but they are likely to have some membrane interaction.Direct experimental evidence of the integration of MPs into the membrane has been obtained only for small hydrophobic MPs that do not belong to the 30K superfamily. There are two TM segments in the p9 protein of carnation mottle virus (41), whereas the p6 protein of beet yellow virus (29) and the p7B protein of melon necrotic spot virus (22) have a single TM segment. In viruses with genomes that include three partially overlapping open reading frames, termed the triple-gene block (TGB), all three TGB proteins are required for movement where the two smaller proteins, TGBp2 and TGBp3, are also TM proteins (24). Furthermore, cross-linking experiments with carnation mottle virus p9 protein demonstrated that its membrane insertion occurs cotranslationally in a signal recognition particle-dependent manner and throughout the cellular membrane integration components, the translocon (33, 34).Prunus necrotic ringspot virus (PNRSV) is a tripartite, positive-strand RNA virus in the genus Ilarvirus of the family Bromoviridae. RNAs 1 and 2 encode the polymerase proteins P1 and P2, respectively. RNA 3 is translated into a single 30K-type MP. The coat protein is translated from a subgenomic RNA 4 produced during virus replication.The present study tackled the association of the PNRSV MP with biological membranes. The in vitro translation of model integral membrane protein constructs in the presence of microsomal membranes demonstrated that the hydrophobic region (HR) of the PNRSV MP did not span the membranes. Different biochemical and biophysical experiments suggested that the protein is tightly associated with, but does not traverse, the membrane, leaving both its N- and C-terminal hydrophilic regions facing the cytosol. Finally, a mutational analysis of the HR revealed that both the helicity and hydrophobicity of the region are essential for viral cell-to-cell movement. 相似文献